Korean Journal of Breeding Science :eISSN 2287-5174 / pISSN 0250-3360

Fig. 1.

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The comparison of target nucleotide sequences between FAD3 and FAD7 of perilla and the construct of RNAi vector to suppress the expression of FAD3 gene encoding Δ15 desaturase. A. Blue arrows represent the DNA region, corresponding to 218-281 aa region where the lowest homology between FAD3 and FAD7. B. pF3SiA vector construct. Asterisk demonstrates unique restriction enzyme site. Bar, phosphinothricin acetyltransferase; CmR, chloramphenicol resistance; F3A, antisense FAD3 fragment for RNAi; F3S, sense FAD3 fragment for RNAi; H, HindIII; K, KpnI; LB, left border; P35S, CaMV 35S promoter; RB, right border; RI, EcoRI; S, SacI; T35S, CaMV 35S terminator.

Korean J Breed Sci 2018;50:463-71 https://doi.org/10.9787/KJBS.2018.50.4.463
© 2018 Korean J Breed Sci