Korean Journal of Breeding Science :eISSN 2287-5174 / pISSN 0250-3360

Fig. 1.

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Plant regeneration from immature embryo cultures in maize of Hi IIA genotype transformed with Agrobacterium carrying GUS, GFP, and bar genes. (A) Infected immature embryos. (B) Transgenic embryogenic type II callus formation on selection medium with 3 mg/L bialaphos. (C) Transgenic somatic embryos on maturation medium. (D) and (E) Germinated somatic embryos on regeneration medium. (F) Regenerated plantlets from somatic embryos on regeneration media. (G) Transgenic plantlet in small pots. (H) Transgenic plants in greenhouse. (I) Harvested ears from transgenic T0 plants through artificial self-pollination.

Korean J Breed Sci 2019;51:290-7 https://doi.org/10.9787/KJBS.2019.51.4.290
© 2019 Korean J Breed Sci