Korean Journal of Breeding Science :eISSN 2287-5174 / pISSN 0250-3360

Fig. 2.

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Transgenic verification of regenerated T0 plants of maize. (A) and (B) Transient expression in uninfected and infected immature embryo, respectively. (C) and (D) GUS expression in callus of untransformant and transformant, respectively. (E) and (F) GUS expression in seeds of untransformant and transformant, respectively. (G), (H), and (I) The expression of phosphinothricin acetyltransferase in T0 GUS-bar, GFP-bar, and mPAT transgenic maize plants using PAT test strip, respectively. (J) and (K) The verification of GUS, GFP, and bar gene in transgenic plants through genomic PCR analysis. Lanes: M, molecular size marker; PC, positive control; Wt, non-transgenic wild-type; lanes 1-14 and 1-7, selected transgenic lines, respectively.

Korean J Breed Sci 2019;51:290-7 https://doi.org/10.9787/KJBS.2019.51.4.290
© 2019 Korean J Breed Sci