Korean Journal of Breeding Science :eISSN 2287-5174 / pISSN 0250-3360

Table. 1.

Oligonucleotide primers used in RT-PCR.

Target Acc. no. Primer sequences Expected size (bp)
ABI1 At4g26080 5'-ATGGAGGAAGTATCTCCGGCGATCG-3' 951
5'-ATCGCCAATGGATCTCGACATGGCG-3'
DREB2A At5g05410 5'-ATGGCAGTTTATGATCAGAGTGGAG-3' 879
5'-CCTGTATGAACCGTTGGCAACACTG-3'
FRY At5g63980 5'-ATGGCTTACGAGAAAGAGCTTGATG-3' 884
5'-ACTATAGCACCAGCGACATGGTCC-3'
HKT1 At4g10310 5'-CGCTGTGACGTTGAGACTGTTACTG-3' 916
5'-CCATATGCACTGATAACTTCGAGAG-3'
SOS1 At2g01980 5'-ATGACGACTGTAATCGACGCGACG-3' 727
5'-ACGCCAGACCAATGCCTACAGCTCC-3' 613
BnABI1 XM_013885981 5'-ATGGAGGAAGTATCACCAGCCGTTG-3'
5'-ACGTGTCACCATCGCAGAGCATCGGC-3' 598
BnDREB2A XM_013863571 5'-ATGGCGGTTTATGACCATAGCGGAG-3'
5'-ACTCGCTCAGCCAATCACTGCTTGG-3' 528
BnFRY XM_013787640 5'-ATGTCATCTATAAATTGCTTTCGAAC-3'
5'-ACCTTCAGATGTGCCACAGTCGATG-3' 505
BnSOS1 ACA50526 5'-TGGCGACTGTAATCGACGCGGCGATG-3'
5'-ACAGGATCTGTGGCACTTAAGAGTCC-3' 864
BrATL30 XM_009141674 5'-ATGCCGATAACGAAACCGCTCGGATC-3'
5'-TTAACTTTGGGATTCCGGTACAAGTG-3' 954
BrZHD10 XM_009103253 5'-ATGGATATGGCGACTCATACAACAA-3'
5'-TCACGACGACGACGACCCGTTTACG-3' 1220
AtActin At3g18780 5'-ATGGCTGAGGCTGATGATATTCAA-3'
5'-TTAGAAACATTTTCTGTGAACGATT-3' 516
Bactin 5'-TGGCATCACACTTTTCTACAA-3'
5'-CAACGGAATCTCTCAGCTCC-3' 961
HPT 5'-AGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTC-3'
5'-TCTACACAGCCATCGGTCCAGACGGCCGCGCTTCTG-3'
Gw-S 5'-ACAAGTTTGTACAAAAAAGCAGGCT-3'
5'-ACCACTTTGTACAAGAAAGCTGGGT-3'
Korean J. Breed. Sci. 2020;52:297-309 https://doi.org/10.9787/KJBS.2020.52.4.297
© 2020 Korean J. Breed. Sci.