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Analysis condition of glycyrrhizic acid and liquiritigenin in licorice.
| HPLC | Agilent 1260 series | ||
|---|---|---|---|
| Column | YMC-PACK ODS-AM (205 mm x 4.6 mm, 5 |
||
| Wavelength | 254 nm (Glycyrrhizin) and 276 nm (Liquiritigenin) | ||
| Mobile phase | Solvent A : 0.1(v/v)% formic acid in distilled water | ||
| Solvent B : 0.1(v/v)% formic acid in acetonitrile | |||
| Flow rate | 1 mL/min | ||
| Column temperature | 40 °C | ||
| Injection volume | 5 |
||
| Gradient elution | Time (min) | Solvent A (%) | Solvent B (%) |
| 0-3 | 95 | 5 | |
| 3 | 95 | 5 | |
| 40 | 0 | 100 | |
| 50 | 0 | 100 | |