Korean Journal of Breeding Science :eISSN 2287-5174 / pISSN 0250-3360

Table. 1.

Analysis condition of glycyrrhizic acid and liquiritigenin in licorice.

HPLC Agilent 1260 series
Column YMC-PACK ODS-AM (205 mm x 4.6 mm, 5 μm)
Wavelength 254 nm (Glycyrrhizin) and 276 nm (Liquiritigenin)
Mobile phase Solvent A : 0.1(v/v)% formic acid in distilled water
Solvent B : 0.1(v/v)% formic acid in acetonitrile
Flow rate 1 mL/min
Column temperature 40 °C
Injection volume 5 μL

Gradient elution Time (min) Solvent A (%) Solvent B (%)

0-3 95 5
3 95 5
40 0 100
50 0 100
Korean J. Breed. Sci. 2020;52:454-9 https://doi.org/10.9787/KJBS.2020.52.4.454
© 2020 Korean J. Breed. Sci.