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Assessment of gene flow from insect-resistant genetically modified rice (Agb0101) to non-GM rice
해충저항성 유전자변형 벼(Agb0101) 유전자 이동성 평가
Korean J. Breed. Sci. 2017;49(3):180-189
Published online September 1, 2017
© 2017 Korean Society of Breeding Science.

Sung-Dug Oh1, Doh-Won Yun1, Soo-In Sohn1, Soon Ki Park2, and Ancheol Chang1*
오성덕1, 윤도원1, 손수인1, 박순기2, 장안철1*

1National Institute of Agricultural Science, Rural Development Administration, Jeonju, 54874, Korea2School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Korea
1농촌진흥청 국립농업과학원, 2경북대학교 응용생명과학부
Genetically modified (GM) crops have been developed worldwide through the recombinant DNA technology and commercialized by global agricultural companies. Until now, GM crops have not been cultivated commercially in Korea. Commercialization of GM crops requires a compulsory assessment of environmental risk associated with the release of GM crops. This study was conducted to evaluate the frequency of pollen mediated gene flow from Bt transgenic rice (Agb0101) to japonica non-GM rice (Nakdongbyeo), indica non-GM rice (IR36), and weedy rice (R55). A total of 729,917, 596,318 and 230,635 seeds were collected from Nakdongbyeo, IR36, and R55, respectively, which were planted around Agb0101. Selection of the hybrids was determined by repeated spraying of herbicide and Cry1Ac1 immunostrip assay. Finally, the hybrids were confirmed by PCR analysis using specific primer. The hybrids were found in all non-GM rice and out-crossing ranged from 0.0005% at IR36 to 0.0027% at Nakdongbyeo. All of hybrids were located within 1.2 m distance from the Agb0101 rice plot. The meteorological elements including rainfall and temperature during rice flowering time were found to be important factors to determine rice out-crossing rate. Consideration should be taken for many factors like the meteorological elements of field and physiological condition of crop to set up the safety management guideline to prevention of GM crops gene flow.
Keywords : Biosafety, Bt transgenic rice, Gene flow

December 2018, 50 (4)