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Assessment of the Candidate Genes of Expression Markers Associated with Drought Stress in Maize Seedlings
옥수수 유묘기 한발 스트레스 평가를 위한 발현마커 후보군 탐색
Korean J Breed Sci 2018;50(3):224-235
Published online September 1, 2018
© 2018 Korean Society of Breeding Science.

Jun-Cheol Moon1, Seungho Shin2, Hyo Chul Kim2, Kitae Song2, Jae Yoon Kim3, Kyung-Hee Kim2, and Byung-Moo Lee2*
문준철1, 신승호2, 김효철2, 송기태2, 김재윤3, 김경희2, 이병무2*

1Agriculture and Life Sciences Research Institute, Kangwon National University, Chuncheon, 24341 Korea
2Department of Life Science, Dongguk University-Seoul, Seoul, 04620, Korea
3Department of Plant Resources, Kongju National University, Yesan, 32439, Korea
1강원대학교 농업생명과학연구원, 2동국대학교 생명과학과, 3공주대학교 식물자원학과
Correspondence to: (E-mail:, Tel: +82-31-961-5130, Fax: +82-31-961-5108)
Received August 8, 2018; Accepted August 14, 2018.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Drought stress during the seedling stage has a disastrous effect on the growth of maize. The purpose of this study was to assess the developed expression markers that are related to drought stress in maize. For the selection of expressed genes by drought stress, co-expression analysis was carried out using published microarray data of drought stress in maize (Zea mays L.) seedlings. Six consensus modules were based on 4,770 stress responsive genes differentially expressed in drought stress, and the royal blue module was chosen. Thirty genes were selected according to different expression patterns between susceptible and tolerant types. Drought stress treatments were performed on both Ki3 and Ki11. Ki3 and Ki11 are widely known drought-susceptible and -tolerant types, respectively. At first, the 30 selected genes were compared to Ki3 and Ki11 using qRT-PCR. The gene expression values of eight genes (BU050895, BF728598, CK827168, CO524848, AF457983, CF037152, AJ606944, and BG836522) were significantly tolerant types rather than susceptible types in the roots. After applying the eight above-mentioned genes to nine cultivars, a different pattern was detected between susceptible and tolerant types. The results of the present study will show the possibility of developing novel expression markers and the application for various maize varieties.
Keywords : Co-expression, Drought stress, Maize seedling, Susceptible type, Tolerant type

September 2018, 50 (3)
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