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Screening and Identification of Omega-5 Gliadin Mutants in Wheat Doubled-Haploid Lines
밀 반수체 계통에서 오메가-5 글리아딘 돌연변이의 선발 및 동정
Korean J Breed Sci 2018;50(3):181-192
Published online September 1, 2018
© 2018 Korean Society of Breeding Science.

You-Ran Jang1, Chon-Sik Kang2, Sun-Hyung Lim1, and Jong-Yeol Lee1*
장유란1, 강천식2, 임선형1, 이종열1*

1National Institute of Agricultural Science, RDA, Jeonju, 54874, Korea
2National Institute of Crop Science, RDA, Jeonju, 55365, Korea
1농촌진흥청 국립농업과학원, 2농촌진흥청 국립식량과학원
Correspondence to: (E-mail: jy0820@korea.kr, Tel: +82-63-238-4616, Fax: 063-238-4604)
Received April 13, 2018; Accepted May 19, 2018.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Gliadin proteins are a major component of gluten proteins and important determinants of bread-making quality by conferring the viscosity and extensibility of dough, but also present significant health problems for consumers with wheat-related diseases like celiac disease or wheat allergies. In order to solve this problem, we conducted RP-HPLC analysis to profile gliadin fractions for screening the mutants deficient in gliadins from 122 wheat doubled-haploid (DH) lines cultivated by the National Institute of Crop Science. Comparing the RP-HPLC chromatogram of 122 DH lines with those of the respective parents, we found that some peaks of omega-5 gliadin were not present in 28 DH lines. Further analysis using SDS-PAGE and A-PAGE showed that the omega-5 gliadin in the parental varieties had two to three bands, but only one band in the absent 28 DH lines. The relative expression levels of all gliadin groups in the parental and mutant lines were also examined by RP-HPLC. Our study contributes to establishing a method for the rapid screening and identification of mutants missing gliadins as major epitopes of wheat-related disease in many wheat genetic resources and breeding lines as valuable information to other researchers.
Keywords : allergen-reduced wheat, gliadin proteins, mutant, omega-5 gliadin, wheat


September 2018, 50 (3)
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