search for




 

Identification of Constitutive Promoters Derived from Brassica rapa
배추 유래 전신발현 프로모터의 동정
Korean J Breed Sci 2018;50(3):193-202
Published online September 1, 2018
© 2018 Korean Society of Breeding Science.

Jin Sun Kim§, Sun-Hyung Lim§, Young-Mi Kim, and Jong-Yeol Lee*
김진선§, 임선형§, 김영미, 이종열*

National Institute of Agricultural Science, RDA, Jeonju, 54874, Korea
농촌진흥청 국립농업과학원
Correspondence to: (E-mail: jy0820@korea.kr, Tel.: +82-63-238-4616, Fax: +82-63-238-4604)
Received June 20, 2018; Accepted August 8, 2018.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Promoters are essential regulatory elements for efficiently expressing a gene of interest in a target tissue of a organism. Therefore, the identification of a suitable promoter is important in plant biotechnology. In this study, four promoters were selected and identified to be constitutively or tissue-specifically expressed in Brassica rapa bacterial artificial chromosome (BAC) clones using the results of transcriptomic analysis of Brassica rapa and open-source database information on Arabidopsis thaliana. The 2 kb region of the 5′ upstream was isolated from the Brassica rapa genomic DNA for each promoter. The four promoters were then fused to β-glucuronidase (gus) and green fluorescent protein (gfp) genes, and the recombinant transgenes were introduced into Arabidopsis. As a result of histochemical GUS staining, GFP fluorescence and RT-PCR, the gus gene was observed to be expressed constitutively in all tissues using all four promoters. A GUS activity assay using fluorescent 4-MUG revealed that the BR11 promoter showed similar activity to the CaMV35S promoter, while the BR4, BR15, and BR16 promoters showed 1.5, 4, and 18-fold higher activities than the CaMV35S promoter, respectively. These results indicate that these four promoters could be used to incorporate useful genes with enhanced function into crops of interest.
Keywords : Brassica rapa; constitutive promoter; GUS


September 2018, 50 (3)
Full Text(PDF) Free

Social Network Service
Services

Cited By Articles
  • CrossRef (0)

Funding Information