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Effects of Overexpression of Brassica rapa GROWTH-REGULATING FACTOR Genes on B. napus Organ Size
배추 GROWTH-REGULATING FACTOR 유전자 발현이 유채 기관크기에 미치는 영향
Korean. J. Breed. Sci. 2018;50(4):378-386
Published online December 1, 2018
© 2018 Korean Society of Breeding Science.

Joon Ki Hong, Eun Jung Suh, Seung-Bum Lee, Hye-Jin Yoon, and Yeon-Hee Lee*
홍준기, 서은정, 이승범, 윤혜진, 이연희*

Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration, 370 Nongsaengmyeong-ro, Jeonju-si, Jeollabuk-do, 54874, Repubic of Korea
농촌진흥청 국립농업과학원 농업생명자원부
Correspondence to: E-mail: yhl2222@korea.kr, Tel: +82-63-238-4690, Fax: +82-63-238-4654
Received July 31, 2018; Revised August 6, 2018; Accepted August 24, 2018.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
GROWTH-REGULATING FACTOR (GRF) genes encode plant-specific transcription factors and play critical roles in regulating the growth and development of lateral organs. In order to explore the agricultural potential of Brassica rapa GRF genes (BrGRFs), we constructed two BrGRF-overexpressing B. napus plants (BrGRF3-1OX and -9OX). BrGRF3-1OX and -9OX developed larger cotyledons, leaves, and seeds than the wild type. The increased organs’ sizes were due to increases in cell number, but not due to cell size alterations. RT-PCR analysis revealed that BrGRFs regulated the expression of a wide range of genes that are involved in gibberellin-, auxin-, cell division-related growth processes. Taken together, our data indicate that BrGRFs act as positive regulators of B. napus growth, thus raising the possibility that they may serve as a useful genetic source for crop improvement with respect to organ size and seed production.
Keywords : Cell division, Cell number, GROWTH-REGULATING FACTOR, Organ size, Transcription factor


December 2018, 50 (4)
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