search for




 

Identification of DNA Markers Related to Resistance to Herbicide Containing Mesotrione in Tongil Type Rice
통일형 벼에서 메소트리온계 제초제 저항성 연관 DNA marker 탐색
Korean. J. Breed. Sci. 2018;50(4):387-395
Published online December 1, 2018
© 2018 Korean Society of Breeding Science.

Ji-Yoon Lee*, Jun-Hyeon Cho, Jong-Hee Lee, Su-Min Cho, Young-Ho Kwon, Dong-Soo Park, You-Chun Song, and Jong-Min Ko
이지윤*, 조준현, 이종희, 조수민, 권영호, 박동수, 송유천, 고종민

Department of Functional Crop, NICS, RDA, Miryang, 50424, Republic of Korea
농촌진흥청 국립식량과학원 남부작물부
Correspondence to: E-mail: minitia@korea.kr, Tel: +82-55-350-1164, Fax: +82-+82-55-352-3059
Received July 31, 2018; Revised August 18, 2018; Accepted October 16, 2018.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
This study was conducted to identify DNA markers related to resistance to herbicide containing mesotrione in Tongil type rice. Two Tongil type elite lines; Milyang154 and Suweon382, showed resistance to mesotrione, whereas the others were susceptible at 20 days after mesotrione application, and severe growth inhibition was observed in the remaining 13 lines. As a result of analysis of mesotrione resistance using 190 F2 populations derived from a cross of Hanareum2 (susceptible) and Milyang154 (resistant), the mesotrione resistance locus was shown to be a single dominant gene with a 3:1 segregation ratio (X2=1.19, P=0.31). To identify a DNA marker closely linked to the mesotrione resistance gene, bulked segregant analysis (BSA) was adopted. The DNA marker RM3501 was identified on chromosome 2 with a recombinant value of 0.53 to the mesotrione resistance gene. Mst1(t) was located between SSR (simple sequence repeat) markers RM3501 and RM324 with a physical map distance of 10.2 Mb–11.4 Mb on chromosome 2. The band pattern of agarose gel electrophoresis of the SSR marker RM3501 showed the same segregation pattern with respect to mesotrione treatment in 20 Tongil type varieties and a BC2F2 segregation population derived from a cross between Unkwang (resistant) and Hanareum2 (susceptible). Thus, the RM3501 DNA marker could be used in breeding programs for Marker Assisted Selection in mesotrione resistant rice breeding.
Keywords : Tongil type rice, Herbicide resistance, Mesotrione, Bulk segregant analysis, Marker assisted selection


December 2018, 50 (4)
Full Text(PDF) Free

Social Network Service
Services

Cited By Articles
  • CrossRef (0)

Funding Information