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Identification and Chromosomal Reshuffling Patterns of Soybean Cultivars Bred in Gangwon-do using 202 InDel Markers Specific to Variation Blocks
변이영역 특이 202개 InDel 마커를 이용한 강원도 육성 콩 품종의 판별 및 염색체 재조합 양상 구명
Korean. J. Breed. Sci. 2018;50(4):396-405
Published online December 1, 2018
© 2018 Korean Society of Breeding Science.

Hwang-Bae Sohn1**, Yun-Ho Song2**, Su-Jeong Kim1, Su-Young Hong1, Ki-Deog Kim1, Bon-Cheol Koo1, and Yul-Ho Kim1*
손황배1**, 송윤호2**, 김수정1, 홍수영1, 김기덕1, 구본철1, 김율호1*

1Highland Agricultural Research Institute, National Institute of Crop Science, RDA, Pyeongchang, Gangwon-do, Republic of Korea
2Gangwondo Agricultural Research and Extension Services, Chuncheon-si, Gangwon-do, Republic of Korea
1농촌진흥청 국립식량과학원 고령지농업연구소, 2강원도농업기술원 연구개발국 작물연구과
Correspondence to: E-mail: kimyuh77@korea.kr, Tel: +82-33-330-1840, Fax: +82-330-330-1519
** These authors contributed equally to this work.
Received August 23, 2018; Revised August 31, 2018; Accepted October 10, 2018.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The areas of soybean (Glycine max (L.) Merrill) cultivation in Gangwon-do have increased due to the growing demand for well-being foods. The soybean barcode system is a useful tool for cultivar identification and diversity analysis, which could be used in the seed production system for soybean cultivars. We genotyped cultivars using 202 insertion and deletion (InDel) markers specific to dense variation blocks (dVBs), and examined their ability to identify cultivars and analyze diversity by comparison to the database in the soybean barcode system. The genetic homology of “Cheonga,” “Gichan,” “Daewang,” “Haesal,” and “Gangil” to the 147 accessions was lower than 81.2%, demonstrating that these barcodes have potentiality in cultivar identification. Diversity analysis of one hundred and fifty-three soybean cultivars revealed four subgroups and one admixture (major allele frequency <0.6). Among the accessions, “Heugcheong,” “Hoban,” and “Cheonga” were included in subgroup 1 and “Gichan,” “Daewang,” “Haesal,” and “Gangil” in the admixture. The genetic regions of subgroups 3 and 4 in the admixture were reshuffled for early maturity and environmental tolerance, respectively, suggesting that soybean accessions with new dVB types should be developed to improve the value of soybean products to the end user. These results indicated that the two-dimensional barcodes of soybean cultivars enable not only genetic identification, but also management of genetic resources through diversity analysis.
Keywords : Gangwon-do, Soybean, InDel, Barcode, Genetic identification


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