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Construction of a Genetic Map using the SSR Markers Derived from “Wonwhang” of Pyrus pyrifolia
배 ‘원황’(Pyrus pyrifolia) 유전체 해독에 기반한 SSR 마커 개발 및 유전자 지도 작성
Korean. J. Breed. Sci. 2018;50(4):434-441
Published online December 1, 2018
© 2018 Korean Society of Breeding Science.

Ji Yun Lee1,2, Mi-Suk Seo1, So Youn Won1, Kyoung Ah Lim1, Il Sheob Shin3, Dongsu Choi2*, and Jung Sun Kim1*
이지윤1,2, 서미숙1, 원소윤1, 임경아1, 신일섭3, 최동수2*, 김정선1*

1Genomics Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA, JeonJu 54874, Republic of Korea
2Departments of Biology, Kunsan National University, Gunsan, 54150, Republic of Korea
3Postharvest Research Division, National Institute of Horticultural and Herbal Science, RDA, Wanju-gun, 55365, Republic of Korea
1농촌진흥청 국립농업과학원 농업생명자원부 유전체과, 2군산대학교 생물학과, 3농촌진흥청 국립원예특작과학원
Correspondence to: Jung Sun Kim, E-mail: jsnkim@korea.kr, Tel: +82-63-238-4559, Fax: +82-63-238-4552
Dongsu Choi, E-mail: choid@kunsan.ac.kr, Tel: +82-63-469-4583, Fax: +82-63-469-7421
Received September 12, 2018; Revised October 30, 2018; Accepted November 9, 2018.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
High-density genetic linkage mapping is critical for undertaking marker-assisted selection and confirming quantitative trait loci, as well as helping to build pseudomolecules of genomes. We constructed a genetic map using 94 F1 populations generated from the interspecific cross between Korean cultivar “Wonwhang” (Pyrus pyrifolia, NCBI BioSample SAMN05196235) and European cultivar “Bartlett” (Pyrus communis). We designed a total of 24,267 SSR markers based on the genome sequences of “Wonwhang” for this. To select the markers that are linked to the traits important in pear breeding programs, SSR-containing genomic sequences were subjected to nucleotide sequence homology searches, which resulted in 510 SSR markers with high similarity to genes encoding proteins with putative functions such as transcription factors, resistance proteins, flowering time, and regulatory genes. Of these, 70 markers showed polymorphisms in parents and segregating populations and were used to construct a genetic linkage map, together with the unpublished 579 SNPs obtained from genotyping by sequencing analysis. The genetic linkage map covered 3,784.2 cM and the average distance between adjacent markers was 5.8 cM. Seventy SSR markers were distributed across 17 chromosomes with more than one locus.
Keywords : Pear, Pyrus pyrifolia, SSR marker, Linkage map


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