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Development of Perilla frutescens with Low Levels of Alpha-Linolenic Acid by Inhibition of a delta 15 desaturase Gene
Delta 15 desaturase 유전자 억제에 의해 알파리놀렌산 함량이 낮은 들깨 육성
Korean. J. Breed. Sci. 2018;50(4):463-471
Published online December 1, 2018
© 2018 Korean Society of Breeding Science.

Kyung-Hwan Kim1*, Kyeong-Ryeol Lee1, Jung-Bong Kim2, Myoung Hee Lee3, Eungyeong Lee1, Nyunhee Kim1, Hongseok Lee1, Song Lim Kim1, JeongHo Baek1, Inchan Choi1, and Hyeonso Ji1
김경환1*, 이경렬1, 김정봉2, 이명희3, 이은경1, 김년희1, 이홍석1, 김송림1, 백정호1, 최인찬1, 지현소1

1Dept. of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA, Jeonju, 54874, Republic of Korea
2Dept. of Agro-food Resources, National Institute of Agricultural Sciences, RDA, Wanju, 55365, Republic of Korea
3Dept. of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang, 50424, Republic of Korea
1농촌진흥청 국립농업과학원 농업생명자원부, 2농촌진흥청 국립농업과학원 농식품자원부,
3농촌진흥청 국립식량과학원 남부작물부
Correspondence to: E-mail: biopiakim@korea.kr, Tel: +82-63-238-4658, Fax: +82-63-238-4654
Received October 30, 2018; Revised October 30, 2018; Accepted November 7, 2018.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Perilla is an oilseed crop cultivated in Korea since ancient times. Due to the high α-linolenic acid content in perilla, perilla seed oil can easily become rancid. α-Linolenic acid is synthesized by two enzymes, endoplasmic reticulum-localized Δ15 desaturase (FAD3) and chloroplast-localized Δ15 desaturase (FAD7) in vivo. In order to lower the α-linolenic acid content of the seed oil without disturbing plant growth, we tried to suppress the expression of only the FAD3 gene using RNA interference, whilst maintaining the expression of the FAD7 gene. Seventeen transgenic plants with herbicide (BastaTM) resistance were obtained by Agrobacterium-mediated transformation using hypocotyls of perilla plants. The transgenic plants were firstly confirmed by treatment with 0.3% (v/v) BastaTM herbicide, and the expression of FAD3 was measured by Northern blot analysis. The α-linolenic acid content was 10-20%, 30-40%, and 60% in two, seven, and three of the twelve T1 transgenic perilla plants which had enough seeds to be analyzed for fatty acid composition, respectively. Analysis of the fatty acid composition of T2 progeny seeds from T1 plants with the lowest α-linolenic acid content showed that the homozygous lines had 6-10% α-linolenic acid content and the heterozygous lines had 20-26% α-linolenic acid content. It is expected that the reduction in α-linolenic acid content in perilla seed oil will prevent rancidity and can be utilized for the production of high-value functional ingredients such as high γ-linolenic acid.
Keywords : FAD3, Δ15 desaturase, α-linolenic acid, RNAi, Perilla


December 2018, 50 (4)
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