Korean Journal of Breeding Science :eISSN 2287-5174 / pISSN 0250-3360

Fig. 2.

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The schematic diagram shows the two processes of genome editing with CRISPR/Cas9 and CRISPR/dCas9. CRISPR-Cas9 system relies on creating cut nucleotide bond of target DNA with Cas9 has endonuclease activity, while dCas9 activation systems for base editing technique rely on similar components like Cas9 to intended targets. dCas9 is one of Cas9 mutants without endonuclease activity. Base editing technique to irreversible conversing one target DNA base into another have been used dCas9 fused to a cytidine deaminase enzyme.

Korean J Breed Sci 2018;50:364-77 https://doi.org/10.9787/KJBS.2018.50.4.364
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