Korean Journal of Breeding Science :eISSN 2287-5174 / pISSN 0250-3360

Table. 1.

Composition of enzyme solutions for protoplast isolation.

Enzyme
compositions
Enzyme solutions
E1 E2 E3 E4 E5 E6
[%, W/V] Cellulase (O)z 0.5 1 2 - - -
Cellulase (T)y - - - 0.25 0.5 1
Pectinase (R)x 0.125 0.25 0.5 - - -
Pectinase (A)w - - - 0.25 0.5 1
Viscozyme - - - 0.5 1 2
mM CaCl2⋅2H2O - - - 10 10 10
CuSO4⋅5H2O - - - 1.6×10-4 1.6×10-4 1.6×10-4
KCl 20 20 20 - - -
KH2PO4 - - - 0.2 0.2 0.2
KI - - - 0.96×10-3 0.96×10-3 0.96×10-3
KNO3 - - - 1 1 1
Mannitol 400 400 400 500 500 500
MgSO4⋅7H2O - - - 1 1 1
MES 20 20 20 3 3 3
pH 5.7 5.7 5.7 5.8 5.8 5.8

zCellulase (O), Cellulase Onozuka R-10; yCellulase (T), Cellulase from Trichoderma reesei (Celluclast); xPectinase (R), Pectinase from Rhizopus sp.; wPectinase (A), Pectinase from Aspergillus aculeatus (Pectinex® Ultra SPL)

Korean J. Breed. Sci. 2021;53:230-9 https://doi.org/10.9787/KJBS.2021.53.3.230
© 2021 Korean J. Breed. Sci.