Korean Journal of Breeding Science :eISSN 2287-5174 / pISSN 0250-3360

Fig. 4.

Download original image
Transfection efficiency of soybean hypocotyl protoplasts with different amounts of plasmid DNA. Protoplasts were transformed with various concentration of the plasmid pCambia1304 harboring a GFP reporter gene: 5 µg (A), 10 µg (B), 20 µg (C) and 40 µg (D). (E) Transfection efficiency from A to D. Fluorescence signal of GFP was examined 24 hours after transformation under microscopy. Scale bars = 40 µm. The error bars indicate the ±standard deviation of three replicates. Different letters indicate a significant difference determined by one-way ANOVA followed by Duncan post hoc test (p < 0.05).
Korean J. Breed. Sci. 2021;53:230-9 https://doi.org/10.9787/KJBS.2021.53.3.230
© 2021 Korean J. Breed. Sci.