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Descriptions of candidate DEGs and primer design for gene expression analysis
| Genes (Assembled transcripts) |
Gene description | Forward (5' → 3') | Reverse (5' → 3') | Tm |
|---|---|---|---|---|
| K1 vs KM1 Up-regulation DEGs | ||||
| Keumgang1SL003317t014 | PROLM24 - Prolamin precursor | AGCAGCAAGGGCAAAGTTTC | TGTCATGGGGATGCTGTAGATG | 60℃ |
| Keumgang1SL001254t019 | beta-amylase | GCCCTTCTCGAATTTGTTGTCG | ACGCAAGCCAGTGTTTGTAG | 60℃ |
| Keumgang1SL095837t001 | ANAC087, Arabidopsis NAC domain containing protein 87 | TCGTCAACCCACATTGCATG | TATTGGAAGCGGCATTTGCG | 60℃ |
| Keumgang1SL002056t010 | MFT, PEBP family protein | AACAGCACCAGCACGTAGC | TGGCTGGTGGTTAACATACCTG | 60℃ |
| Keumgang1SL008016t001 | Serine protease inhibitor, potato inhibitor I-type family protein | AGCAAGCTTGATGTGCATGG | GGAAAGTACATCCATGGCATGC | 60℃ |
| Keumgang1SL001264t011 | ATCRA1,CRA1,CRU1, RmlC-like cupins superfamily protein | AGTGGTGTAGATGCTTGTAGCC | TGGCACAACTGTTTGGCATG | 60℃ |
| Keumgang1SL034539t010 | Tubby-like F-box protein | AAAGATGTCGTCGCTTGTGC | CGACACCGGAAAAGTGAGTTTC | 60℃ |
| Keumgang1SL011205t005 | ATSUC3,ATSUT2,SUC3,SUT2, sucrose transporter 2 | ACCTTGCACAGTGTTGTTGG | TCTGCATTGCTGTTGTGGTC | 60℃ |
| K1 vs K7 Up-regulation DEGs | ||||
| Keumgang1SL003578t009 | Starch branching enzyme 2.2 | GCTCTGATGTTTGGTGGACATG | TTCCGCTTTTTCCTCAACGC | 60℃ |
| Keumgang1SL001971t019 | BETA-VPE,BETAVPE, vacuolar-processing enzyme precursor | TCGCCAAAAACGACCTCAAC | AGTAACCGCGGTTTTGTTCC | 60℃ |
| Keumgang1SL003424t003 | fatty acid hydroxylase | TTTCCAGTTCTCCCTCCATTCG | ATTTGACGGTTCCCACATGC | 60℃ |
| Keumgang1SL014234t009 | 4-alpha-glucanotransferase | TGCTGGTTTTTGGGCAGTTC | ACAGCTTTGAACAGCGCATC | 60℃ |
| Keumgang1SL007804t008 | ACT domain containing protein | TATTGCGGAGATTGAGGGCTAC | TTGCATTTGAGGCTCGCAAG | 60℃ |
| Keumgang1SL000284t005 | AtCOR47,COR47,RD17, cold-regulated 47 | CAAACACAACACACGCCAAC | TGCCTGGTTACCACAAGACAG | 60℃ |
| Keumgang1SL004791t001 | thiaminC, thiamine biosynthesis protein | TGTGTGGTCCCAAGTTTTGC | AGCTTCACCATGTTGTTCGC | 60℃ |
| Keumgang1SL038902t003 | CAMK includes calcium/ calmodulin depedent protein kinases | AGAACCTCATGTCCATGTACCG | TTCCAAGAGAGGGTTCGGAATG | 60℃ |
| Keumgang1SL007502t020 | zinc finger, C3HC4 type domain containing protein | ACAATGGGCATGCCAAATGG | ACATTGCTTTGACGCTGAGG | 60℃ |
| Keumgang1SL000284t005 | aldehyde dehydrogenase | AACCACTTTGACTGCAGTGC | ATGCTTGCATTGTGCTGGAC | 60℃ |
| Housekeeping gene | ||||
| Housekeeping gene | GCTGTTCCAGCCATCTCATGT | CGATCAGCAATTCCAGGAAAC | 60℃ | |