Korean Journal of Breeding Science

Sequence of primers for identification of Korean wheat cultivars and expected PCR fragment size

Gene	Marker typez	Primer	Sequence (5'→3')	Fragment size (bp)	Reference
Glu-A1c	STS	AxNull-F	ACGTTCCCCTACAGGTACTA	920	(25)
Glu-A1c		AxNull-R	TATCACTGGCTAGCCGACAA	920	(25)
Glu-A3ac		Glu-A3ac-F	AACAGAATTATTAAAGCCGG	573	(51)
Glu-A3ac		Glu-A3ac-R	CTGTGCTTGGATGATACTCTA	573	(51)
Glu-B1b		ZSBy85F5	TTAGCGCTAAGTGCCGTCT	527	(28)
Glu-B1b		ZSBy85R5	TTGTCCTATTTGCTGCCCTT	527	(28)
Glu-B3h		Glu-B3h-F	CCACCACAACAAACATTAA	1022	(52)
Glu-B3h		Glu-B3h-R	GTGGTGGTTCTATACAACGA	1022	(52)
Lr34		csLV34 F	GTTGGTTAAGACTGGTGATGG	229/150	(50)
Lr34		csLV34 R	TGCTTGCTATTGCTGAATAGT	229/150	(50)
Pina-D1	STS	Pina-D1F	ATGAAGGCCCTCTTCCTCA	447	(13)
Pina-D1		Pina-D1R	TCACCAGTAATAGCCAATAGTG-	447	(13)
Ppo-A1		PPO18F	AACTGCTGGCTCTTCTTCCCA	685/876	(49)
Ppo-A1		PPO18R	AAGAAGTTGCCCATGTCCGC	685/876	(49)
Ppo-B1		F-8F	ACCTTGACTCCAGTGGGACC	400+600/400	(43)
Ppo-B1		F-8R	CCGAGTAGAAGTTGCCCAT	400+600/400	(43)
Rht-B1b		NH-BF.2	TCTCCTCCCTCCCCACCCCAAC	360	(10)
Rht-B1b		MR1	CATCCCCATGGCCATCTCGAGCTA	360	(10)
TaCwi-A1b		CWI21F	GTGGTGATGAGTTCATGGTTAAG	404	(30)
TaCwi-A1b		CWI21R	AGAAGCCCAACATTAAATCAAC	404	(30)
Vrn-D1a		Intr1/D/F	GTTGTCTGCCTCATCAAATCC	1671	(11)
Vrn-D1a		Intr1/D/R3	GGTCACTGGTGGTCTGTGC	1671	(11)
-	SCAR	KWSM003F	TGCTGAGGGCATATCTCAACA	682	(46)
-	SCAR	KWSM003R	GTGTTGCACGACGCTGTACT	682	(46)
-	SSR	TaSE96F	TGGGACAAGTCCCTAGGTAAGACG	295/313-317	(12)
-	SSR	TaSE96R	GTAGTCCGCCCAGCCTCTACTTTT	295/313-317	(12)

^zSTS = sequence-tagged site, SCAR = Sequence characterized amplified region, SSR = simple sequence repeat.