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Conditions of multiplex-PCR for identification of government certificated wheat cultivar in Korea
| Multiplex-PCR | |
|---|---|
| Allele | |
| PCR reaction components | |
| Taq DNA polymerase (U) | 4 |
| Each dNTP (mmol/l) | 3 |
| Primer (μmol/l) | 1.5/1/0.5 |
| Concentration of DNA (ng) | 100 |
| PCR amplification condition | |
| Pre-denaturation | 94℃, 5 m |
| Denaturation | 94℃, 1 m |
| Annealing | 64℃, 1 m |
| Extension | 72℃, 1 m |
| No. of cycles | 33 |
| Final extension | 72℃, 5 m |
| Gel electrophoresis | |
| Agarose (%) | 1.5 |
| Voltage (V) | 135 |
| Time (h) | 1 |
zConcentrations of primers for