Korean Journal of Breeding Science :eISSN 2287-5174 / pISSN 0250-3360

Table. 4.

Conditions of multiplex-PCR for identification of government certificated wheat cultivar in Korea

Multiplex-PCR
Allele Glu-A1b/Wx-B1a/Glu-D1d
PCR reaction components
Taq DNA polymerase (U) 4
Each dNTP (mmol/l) 3
Primer (μmol/l) 1.5/1/0.5z
Concentration of DNA (ng) 100
PCR amplification condition
Pre-denaturation 94℃, 5 m
Denaturation 94℃, 1 m
Annealing 64℃, 1 m
Extension 72℃, 1 m
No. of cycles 33
Final extension 72℃, 5 m
Gel electrophoresis
Agarose (%) 1.5
Voltage (V) 135
Time (h) 1

zConcentrations of primers for Glu-A1b, Wx-B1a and Glu-D1d, respectively.

Korean J. Breed. Sci. 2022;54:119-29 https://doi.org/10.9787/KJBS.2022.54.2.119
© 2022 Korean J. Breed. Sci.