PCR analysis to confirm disease resistance genes (A) and grain-related genes (B) using gene specific DNA marker. Disease resistance genes, Xa3, Xa21, and Stvb-i were confirmed by PCR products amplified with primer, 9643.T4 (cleaved by Taqα Ⅰ), U1/I1, and InDel 7, respectively. Grain-related genes, GW2, GS3, and qSW5 were confirmed by PCR products amplified with primer, GW2-Hpa Ⅰ (cleaved by Hpa Ⅰ), GS3-Pst Ⅰ (Pst Ⅰ), and N1212del, respectively. M: DNA size marker, 1: ‘Nampyeong’, 2: ‘Sindongjin’, 3: ‘Peyongan’, 4: ‘Dami’, 5: ‘HR27195’, 6: ‘Chamdongjin’.

Korean J. Breed. Sci. 2023;55:86-102 https://doi.org/10.9787/KJBS.2023.55.1.86

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