Korean Journal of Breeding Science

Primers used in this study for detection of Fusarium head blight resistance genes.

Gene	Primer name	5'-3'	AT^z (℃)	Agarose gel (%)	Genome	Reference
Fhb1	TaHRC-GSM-F	ATTCCTACTAGCCGCCTGGT	68	1.2	3B	Su et al. 2019
	TaHRC-GSM-R	ACTGGGGCAAGCAAACATTG	68	1.2	3B	Su et al. 2019
	SGNH_GSP-F	CAATTCCAGCAGTTCATCAAC	68	2.0	3B	Singh et al. 2019
	SGNH_GSP-R	CATACCCGCAACACACAT	68	2.0	3B	Singh et al. 2019
Fhb2	Xwmc398-F	GGAGATTGACCGAGTGGAT	66	2.0	6B	Cuthbert et al. 2007
	Xwmc398-R	CGTGAGAGCGGTTCTTTG	66	2.0	6B	Cuthbert et al. 2007
	Xwmc397-F	AGTCGTGCACCTCCATTTTG	68	2.0	6B	Cuthbert et al. 2007
	Xwmc397-R	CATTGGACATCGGAGACCTG	68	2.0	6B	Cuthbert et al. 2007
Fhb4	Xgwm349-4B-F	ACACACACTCGATCGCAC	66	3.0	4B	Xue et al. 2010
	Xgwm349-4B-R	GCAGTTGATCATCAAAACACA	66	3.0	4B	Xue et al. 2010
	Xgwm149-F	CATTGTTTTCTGCCTCTAGCC	68	2.0	4B	Somers et al. 2003, Xue et al. 2011
	Xgwm149-R	CTAGCATCGAACCTGAACAAG	68	2.0	4B	Somers et al. 2003, Xue et al. 2011
Fhb5	Xgwm304-F	AGGAAACAGAAATATCGCGG	68	2.0	5A	Xue et al. 2011
	Xgwm304-R	AGGACTGTGGGGAATGAATG	68	2.0	5A	Xue et al. 2011
	Xwmc705-F	GGTTGGGCTCCTGTCTGTGAA	68	2.0	5A	McCartney et al. 2004
	Xwmc705-R	TCTTGCACCTTCCCATGCTCT	68	2.0	5A	McCartney et al. 2004
	Xgwm156-F	CCAACCGTGCTATTAGTCATTC	68	2.0	5A	Jing et al. 2007
	Xgwm156-R	CAATGCAGGCCCTCCTAAC

^zAT; annealing temperature.