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Primer information used in this study.
| PS | Gene | Primer name | sequence (5'-3') | AT (℃) | Agarose gel (%) | Reference |
|---|---|---|---|---|---|---|
| 1 | MHBx717-F | CACTGAGATGGCTAAGCGCC | 66 | 1 | Lee et al. 2024 | |
| MHBx717-R | GATCTTGTTGCCCTTGTCC | |||||
| 2 | Bx7F | CAACTTCTTCACAGCAGT | 60 | 2 | Espi et al. 2012 | |
| Bx7R (*) | TGCGCCTTTGCCACCTTTAG | |||||
| 3 | cauBx642-F | GGGCAATCGGGGTACTTCC | 60 | 1 | Xu et al. 2008 | |
| cauBx642-R | CCCTTGTCTTGGCTGTTGTC | |||||
| 4 | MHBx14-Ha-F | TACTACCCAACCTCTCCACA | 64 | 3 | Lee et al. 2024 | |
| MHBx14-Ha-R | CTGTTGCTCTTGTGCTGATTG | |||||
| 5 | MAR-F | CCTCAGCATGCAAACATGCAGC | 66 | 1 | Butow et al. 2003 | |
| MAR-R | CTGAAACCTTTGGCCAGTCATGTC | |||||
| 6 | MHBx13-F | GGCACGAGGCAATACGAGCG | 66 | 1 | Lee et al. 2024 | |
| MHBx13-R | TGATCTTGTTGCTCTTGTCT | |||||
| 7 | MHBx13pro-F | GGGCTTTAGGAGAGATGGTTTAT | 60 | 1 | Lee et al. 2024 | |
| MHBx13pro-R | GCCTATGAAGAAAGCGTGAGA | |||||
| 8 | MHBx-185-F | GATAAGGCCAACGAGAGAAGAA | 60 | 1 | Lee et al. 2024 | |
| MHBx-185-R | GATCTTAGTAAATCCGCGTCAAATAG | |||||
| 9 | cauBx752-F | AGGGGCAGGGAAGAAACACT | 66 | 1 | Xu et al. 2008 | |
| cauBx752-R | CCAGGCAACACAAATCCATG | |||||
| 10 | MHBx20-F | GTGTACTACCCAACTTCTCT | 66 | 1 | Lee et al. 2024 | |
| MHBx20-R | CCTGGCTGTTGCTCATGTCT | |||||
| 11 | MHBx6-F | CACTGAGATGGCTAAGCGCC | 67 | 1 | Lee et al. 2024 | |
| MHBx6-R | GATCTTGTTGCCCTTGTTC | |||||
| 12 | ZSBy8F5 | TTAGCGCTAAGTGCCGTCT | 69 | 1 | Lei et al. 2006 | |
| ZSBy8R5 | TTGTCCTATTTGCTGCCCTT | |||||
| 13 | By18-SNP-F | TTAGCGCTAAGTGCCGTCC | 66 | 1 | Lei et al. 2006 | |
| By18-SNP-R | TTGTCCTATTTGCTGCCCTT | |||||
| 14 | By01-F | GGGGCCATATTATCCAGGCC | 66 | 1 | Frank et al. 2017 | |
| By02-R | TTTGTCCTAGCTGTCCTATTTGT | |||||
| 15 | ZSBy9aF1 | TTCTCTGCATCAGTCAGGA | 60 | 1 | Lei et al. 2006 | |
| ZSBy9aR3 | AGAGAAGCTGTGTAATGCC | |||||
| 16 | MHBy16-F | GGGCAGCAAATAGGACAAGA | 68 | 1 | Lee et al. 2024 | |
| MHBy16-R | TTGTCCTACCTGCTGCAGG | |||||
| 17 | MHBy18-F | CAACTTCTCCACAACAGTCG | 68 | 1 | Lee et al. 2024 | |
| MHBy18-R | GTCCTAGTTGGTGTCCTTGTT |
PS, primer set; AT, annealing temperature.