Comparison of protoplast viability by using different methods of hypocotyl sample preparation. (A) Hypocotyls cut by 0.5-1 mm in length. (B) Hypocotyls cut by 1-2 cm in length and longitudinally in half. Bright field image (C) and fluorescence field image (E) of protoplast isolated from hypocotyls cut by 0.5-1 mm in length. Bright field image (D) and fluorescence field image (F) of protoplast isolated from hypocotyls cut by 1-2 cm in length and longitudinally in half. All images are observed under Confocal Laser Scanning Microscopy (CLSM) at 20X magnification. Viable protoplasts are stained by FDA. Fluorescence of FDA is shown in green. The scale bar is 40 µm.

Korean J. Breed. Sci. 2025;57:1-11 https://doi.org/10.9787/KJBS.2025.57.1.1

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