Protoplast isolation efficiency and viability by using different methods of hypocotyl sample preparation. (A) Quantification of protoplast counts per fresh tissue weight (FW) (g). (B) Viability of protoplast (%). Previous method: hypocotyls cut by 0.5-1 mm in length, improved method: hypocotyls cut by 1-2 cm in length and longitudinally in half. The error bars indicate the standard deviation of three replicates. One-tailed Student’s t-tests were conducted to test the differences between the previous and improved methods statistically (*p-value<0.05).

Korean J. Breed. Sci. 2025;57:1-11 https://doi.org/10.9787/KJBS.2025.57.1.1

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