Comparison of protoplast viability by using different methods of protoplast purification. Bright field image (A) and fluorescence field image (C) of protoplast isolated without sucrose density gradients purification. Bright field image (B) and fluorescence field image (D) of protoplast isolated with sucrose density gradients purification. Red points indicate cell wall and dead cell debris. All images are observed under Confocal Laser Scanning Microscopy (CLSM) at 20X magnification. Viable protoplasts are stained by FDA. Fluorescence of FDA is shown in green. The scale bar is 40 µm.

Korean J. Breed. Sci. 2025;57:1-11 https://doi.org/10.9787/KJBS.2025.57.1.1

© 2025 Korean J. Breed. Sci.