The grain size and shape have a significant impact on the grain quality and yield of rice. Additionally, due to the globalization of the rice market, there is a demand for rice varieties of various sizes. In this study, we analyzed quantitative trait loci (QTL) associated with grain length (GL), grain width (GW), grain thickness (GT) and ratio of length-to-width (RLW) using 149 F₂ plants obtained from a cross between Hopum and Basmati 370. To construct a linkage map, we utilized 89 Fluidigm SNP markers, which covered a span of 1,116 cM of F₂ population. Nine QTLs associated with four traits were identified on chromosomes 2, 3, 5, and 7. One major QTL on chromosome 3 accounted for 43.2% of the phenotypic variation in GL. Additionally, QTLs named qGW2 and qGW5 on chromosomes 2 and 5 were detected for GW, explaining 10.4% and 18.6% of the phenotypic variation, respectively. Three QTLs for GT were found on chromosomes 2, 5, and 7, explaining 9.9%, 12.9%, and 9.9% of phenotypic variation, respectively. These QTLs offer potential applications in breeding programs using Basmati 370 to develop long and slender grain japonica varieties.