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The most downloaded articles in the last three months among those published since 2024.

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TCS 기반 SNP 분석을 통한 한국 밥쌀용 벼 품종의 유전적 다양성 및 구조적 네트워크 분석
Genetic Diversity and Structural Network Analysis of Korean Rice Varieties Using TCS-based SNPs
Chang-Min Lee, Hyun-Su Park, Jeonghwan-Seo, Song-Hee Park, O-Young Jeong, Keon-Mi Lee, Seul-Gi Park
Korean. J. Breed. Sci. 2026;58(1):1-12.
Published online March 1, 2026
DOI: https://doi.org/10.9787/KJBS.2026.58.1.1

In the present study, we conducted a detailed analysis of the genetic diversity and structural organization of 96 domestic Korean rice varieties (Oryza sativa L.) using 2,565 high-resolution TCS-based single nucleotide polymorphism (SNP) markers. Genetic structural variations were investigated using diversity indices, PCA, genetic similarity, and network analysis. Genetic diversity analysis revealed a significant expansion of the genetic foundation after the 1980s, marked by a sharp increase in the number of alleles (Na) from the 2000s. Despite this, high genetic homogeneity was maintained, with an average similarity of 77.7%. The observed 10% difference among same-cross varieties suggests that critical genetic variations are fixed by strong selection pressures for quality traits. Network analysis (85% similarity threshold) confirmed that the Korean rice breeding population followed a distinct core-periphery model (eight communities). The connected 84 varieties had a centrality range of 0.01 0.39. Core Variety Groups (e.g., ‘Junam’ and ‘Sindongjin’) exhibited the highest centrality (up to 0.39), indicating their extensive use as key breeding parents and their function as the central axis of the genetic network. Bridge Variety Groups (e.g., ‘Hwayeong’ and ‘Samkwang’) played an intermediary role linking clusters. Crucially, 12 ‘isolated accessions’ showed zero centrality (0.00), representing a genetic disconnect from the main pool. This quantitative network-based assessment provides essential fundamental data for breeders to select appropriate germplasms. Furthermore, the findings suggest that the current cultivar naming system, which inadequately reflects genetic relationships, requires reassessment, and that the establishment of
objective
management standards based on this research is warranted.

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콤바인수확용 내탈립성이 강한 흰색 참깨 ‘하니올’
A White Sesame Variety ‘Haniall’ with Shattering Resistance for Combine Harvest
SungUp Kim, Jeongeun Lee, Jung In Kim, Sang Woo Kim, Eunsoo Lee, Myoung Hee Lee, Eunyoung Oh, Min Young Kim
Korean. J. Breed. Sci. 2026;58(1):63-69.
Published online March 1, 2026
DOI: https://doi.org/10.9787/KJBS.2026.58.1.63

A white sesame variety, ‘Haniall’ (Sesamum indicum L.), exhibiting shattering resistance and early maturity, was developed in 2021. ‘Haniall’ originated from a cross between ‘Suwon195’ and ‘Early Russian’ in 2014. ‘Haniall’ features a plant type characterized many branches and single capsule per node. Notably, the ‘Haniall’ exhibited resistance to seed shattering. The yield of Haniall was about 104 kg/10 a, which is 20% higher than that of ‘Ansan.’ ‘Haniall’ showed crude fat content of 54%, a lignan content of 6.4 mg/g. This variety is expected to contribute to the increased sesame production in Korea through large-scale mechanical cultivation (Registration No. 10320).

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South America, particularly the Southern Cone region, which includes Argentina, Brazil, Paraguay, and Uruguay, serves as a global hub for soybean production, accounting for more than 56% of global supply. However, this region experiences recurrent and severe drought risks driven by climate variability associated with the El Niño-Southern Oscillation (ENSO), resulting in significant economic losses. Consequently, biotechnology- based drought-tolerant soybean breeding has emerged as a critical national strategy across these countries. Argentina has positioned itself as a primary technology originator with the development of the HB4 soybean, the world’s first commercial drought-tolerant trait, utilizing the sunflower-derived transcription factor HaHB4. Brazil is advancing beyond single-trait approaches by developing multistacked biotech varieties that integrate drought tolerance with herbicide resistance, insect resistance, and oxidative stress mitigation. Furthermore, Paraguay and Uruguay are rapidly emerging as international hubs for field testing and commercialization, supported by streamlined regulatory frameworks and a “dual-track” strategy for both genetically modified (GM) and genome-edited (GE) crops. This review integrates molecular strategies, national regulatory systems, and commercialization trends in South America and discusses strategic implications for Korea. Despite Korea’s limited domestic experience in large-scale commercial cultivation owing to regulatory constraints, it possesses internationally competitive expertise in transcription factor engineering, CRISPR-based precision breeding, and AI-driven stress phenotyping. We propose a strategic technology export model that leverages Korea’s advanced R&D capabilities as a “technology provider” and “joint developer,” in partnership with South American countries. Such collaborations can foster a climate-resilient agricultural ecosystem and secure Korea’s position in the global biotech seed market.

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당도가 높은 촉성재배용 딸기 품종 ‘은향’
‘Eunhyang’, A Strawberry Variety for Forcing Culture with High Soluble Solids Contents
Inha Lee, Hyun Sook Kim, Myeong Hyeon Nam, Hee Chul Lee, Je Hyeok Yoo, Donggyeom Kim
Korean. J. Breed. Sci. 2026;58(1):87-95.
Published online March 1, 2026
DOI: https://doi.org/10.9787/KJBS.2026.58.1.87

‘Eunhyang’ is a new strawberry cultivar developed in 2021 by the Strawberry Research Institute of the Chungcheongnam-do Agricultural Research and Extension Services for forcing culture. The cultivar was bred to diversify the domestic market dominated by the single cultivar ‘Sulhyang’ and to improve fruit quality, including sweetness, firmness, and flavor. ‘Daewang,’ characterized by excellent flavor, aroma, and high firmness was used as the female parent, while ‘Durihyang,’ bred by the same institute with large fruit size, strong vigor, and good taste, was used as the male parent. Artificial crossing was conducted in 2017, followed by seedling selection, evaluation of characteristics, and productivity testing from 2018 to 2020. The selected line, initially designated ‘Nonsan 16,’ was officially named ‘Eunhyang’ in 2021. ‘Eunhyang’ exhibits semi-spreading growth, strong plant vigor, and elliptic leaves. Its flowering date is slightly earlier than that of ‘Sulhyang,’ and it shows excellent continuous flowering ability. The first harvest date was similar to that of ‘Sulhyang.’ The fruits are conical and bright light red. The first flower cluster has 8.6 flowers, fewer than ‘Sulhyang’ (16.8), reducing labor for flower thinning. It demonstrates higher soluble solids content (11.0 °Brix), stronger fruit firmness (14.1 g/mm2), and a higher sugar-to-acid ratio (19.0) than ‘Sulhyang.’ The average fruit weight is 17.7 g, which is heavier than ‘Sulhyang’ (15.4 g). Total yield is 3,945 kg/10a, which is 93% of ‘Sulhyang’ (4,236 kg/10a). ‘Eunhyang’ is relatively susceptible to anthracnose, powdery mildew, and two-spotted spider mites. ‘Eunhyang’ was registered for plant variety protection in 2023 (Registration No. 9751).

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Research Article

녹두 품종 별 추출물의 항염증 효능 평가
Anti-Inflammatory Effects of Mung Bean (Vigna radiata) Extracts from Different Cultivars
Yeong Jae An, Hye Young Seo, Min Young Kim, Ji Eun Ra, Seung-Yeob Song
Korean. J. Breed. Sci. 2025;57(4):345-357.
Published online December 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.4.345

Mung bean (Vigna radiata) is a rich natural source of vitexin and isovitexin—flavonoids known for their potential anti-inflammatory properties. The aim of this study was to evaluate, through the use of RAW 264.7 macrophages, the anti-inflammatory effects of extracts from mung bean seeds containing vitexin and isovitexin. High-performance liquid chromatography (HPLC) was employed to quantify the levels of these compounds in various mung bean cultivars: “Jinhwang,” “Samhwang,” “Seonhwa,” and “Jangan,” as well as “Dahyeon,” and “Sanpo,” two of the most widely cultivated varieties in Korea. Cytotoxicity assays revealed no significant toxicity at concentrations of 25, 50, and 100 µg/mL, allowing further analysis at these levels. In nitric oxide (NO) inhibition assays, “Samhwang” (10.61 µM) and “Seonhwa” (9.7 µM) demonstrated the highest NO-suppressing activity at 50 µg/mL. Tumor necrosis factor-alpha (TNF-α) levels were significantly reduced by “Seonhwa” (83.6 pg/mL) and “Jangan” (72.3 pg/mL), with “Jangan” showing the strongest inhibitory effect. Interleukin-6 (IL-6) analysis revealed notable suppression in “Samhwang,” “Seonhwa,” and “Jangan” at 50 µg/mL, with “Samhwang” exhibiting the most potent effect (78.6 pg/mL). These findings suggest that the “Samhwang” cultivar, in particular, possesses significant anti-inflammatory potential and may serve as a valuable candidate for the development of natural anti-inflammatory agents.

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Articles

지역특화 중만생 자포니카 장립종 벼 ‘아미쌀’
‘Amissal’: A Region-specific, Mid-late Maturing Long-grain Japonica Rice Cultivar
Hyun-Su Park, Chang-Min Lee, Ki-Young Kim, O-Young Jeong, Ji-Ung Jeung, Su-Keyong Ha, Sang-Chul Park, Sang-Hyeok Lee, Jung-Pil Suh, Mina Jin, Hyun-Sook Lee, Jeonghwan Seo, Songhee Park, Jae-Ryoung Park, Kyeongmin Kang
Korean. J. Breed. Sci. 2025;57(4):547-563.
Published online December 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.4.547

‘Amissal’ is a regionally specialized rice cultivar developed to strengthen the competitiveness of local rice and is characterized by its distinct long-grain japonica type. It was bred through a single backcross between ‘Boramchan’—a high-yielding japonica cultivar with excellent cultivation stability used as the recurrent parent—and ‘HR30198-AC33’ (DGS79), a breeding stock with extra-long and spindle-shaped grains used as the donor parent for japonica grain shape diversification. Population and pedigree breeding methods were used during the breeding process. During the selection stage, molecular marker-assisted selection targeting the major grain shape genes, GS3 and qSW5 was applied, enabling the identification of lines carrying the gs3-qSW5 allele combination associated with long grain shape. ‘Amissal’ was developed through yield trials, local adaptability tests, selection trials addressing regional needs, and on-farm research aimed at developing long-grain japonica rice for export. The heading date of ‘Amissal’ was August 17, two days later than that of ‘Nampyeong’. Compared to ‘Nampyeong’, ‘Amissal’ exhibited greater biomass due to longer culms, more spikelets per panicle but fewer panicles per hill, and a heavier 1,000-grain weight. It demonstrated strong resistance to bacterial blight (races K1, K2, and K3) but was susceptible to viral diseases and insect pests. In terms of yield, it outperformed ‘Nampyeong’. The average brown rice grain length was 6.14 mm, classifying it as long-grain type. The length-to-width ratio was 2.43, corresponding to a semi-spindle shape, which clearly distinguishes it from existing Korean japonica cultivars. While its milling recovery rate was similar to that of ‘Nampyeong’, a high proportion of broken rice due to its long grain shape resulted in a lower head rice. ‘Amissal’ had low protein content and provided the sticky and soft texture typical of japonica rice, with excellent scores in sensory evaluation tests. As a differentiated, regionally specialized rice cultivar featuring a long-grain japonica type and superior eating quality, ‘Amissal’ is expected to contribute to the advancement of the local rice industry, particularly through applications in rice export and the utilization of broken rice and rice straw (Registration No. 10167).

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열대지역 수출용 다수성 단교잡 종실 옥수수 ‘케이엠7'
‘KM7’: A High-yield Hybrid Corn for Export to Tropical Regions
Hwan-Hee Bae, Young-Sam Go, Jun Young Ha, Jae-Han Son, Tae-Wook Jung, Jung-Tae Kim, Jin-Seok Lee, Gibum Yi, Yoo-Chan Choi, Hi-Che Chung, Seong-Hyu Shin, Ki-Young Kim
Korean. J. Breed. Sci. 2025;57(4):475-481.
Published online December 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.4.475

‘KM7’, a maize hybrid for grain production, was developed for the Southeast Asian seed market by the maize breeding team of the National Institute of Crop Science (NICS), Rural Development Administration (RDA) in 2020. The hybrid, ‘KM7’, was bred by crossing two inbred lines, CL32 and 14K23. After a one-year yield trial in Dong Trieu, Vietnam, country adaptability trials were performed three times in three different countries (Vietnam, Cambodia, and Indonesia) in both 2019 and 2020. The seed type was flint-like and suitable for both food and feed. ‘KM7’ adapted well to conditions in northern Vietnam, northwestern Cambodia, and Central Java in Indonesia. The silking date of ‘KM7’ in Vietnam was 62 d, which was 1 d earler than that of LCH9, a leading cultivar in Vietnam. ‘KM7’ is an early maturing, high-grain yielding F1 hybrid that is also resistant to downy mildew. ‘KM7’ produced a grain yield of 842, 831, and 950 kg/10a in Vietnam, Cambodia, and Indonesia, respectively. This demonstrated a higher yield than that of the leading cultivars in each respective country. ‘KM7’ produced 26% more grain in Vietnam, 28% more in Cambodia, and 56% more in Indonesia than the respective control varieties (Registration No. 10034).

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밀의 면역원성을 줄이기 위한 최신 연구 동향 소개
Latest Research Trends in Reducing the Immunogenicity of Wheat
Sora Lee, Jong-Yeol Lee, Sichul Lee, Jae-Ryeong Sim, Sewon Kim, Woo-Suk Cho
Korean. J. Breed. Sci. 2025;57(2):65-89.
Published online June 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.2.65

Wheat is a fundamental staple crop worldwide, contributing significantly to global food security due to its versatility and nutritional value. However, gluten proteins, including gliadins and glutenins, have been implicated in various health problems, such as celiac disease, non-celiac gluten sensitivity, and wheat allergies. These disorders affect a wide variety of people globally, creating demand for wheat varieties that balance high-end-use quality with reduced immunogenic potential. This review examines the molecular and genetic mechanisms that regulate gluten protein synthesis, highlighting recent advances in genomic and mutagenic approaches aimed at modifying gluten proteins to enhance the health and quality traits of wheat. Technologies such as RNAi and CRISPR/Cas9 offer promising avenues for reducing wheat immunogenicity without compromising its functional properties in food production. This study also examines the challenges and prospects of utilizing these genetic tools to develop wheat varieties that achieve the dual
objective
s of enhanced health outcomes and high product quality.

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콩() 하배축을 이용한 효율적인 원형질체 분리법
An Efficient Protoplast Isolation Method Using Hypocotyl in Soybean (Glycine max)
Jaehwan Kim, Yeong Yeop Jeong, Hyunwoo Park, Pil Joon Seo, Kyung Do Kim
Korean. J. Breed. Sci. 2025;57(1):1-11.
Published online March 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.1.1

Soybean is one of the most important crops because of its high protein and oil content. Previous studies have refined protoplast isolation methods for soybeans to enhance transfection efficiency. However, these methods have limitations due to the inconsistent number of viable protoplasts for various applications. In this study, we propose an optimized protoplast isolation method to overcome this challenge. Hypocotyls grown in the dark were selected to ensure rapid growth and a steady supply of plant materials. The hypocotyls were cut to 1–2 cm in length and halved longitudinally to achieve consistent protoplast yields. Our new hypocotyl cutting method demonstrated 1.5 times improved protoplast yield and improved protoplast viability compared to that of previous methods. The isolated protoplasts were purified using the sucrose density gradient purification method to remove residues while stacking viable protoplasts. Fluorescein diacetate (FDA) staining was performed to determine the proportion of healthy protoplasts throughout the process. Consequently, we propose a new protoplast isolation protocol that ensures a higher yield, better viability, and healthier conditions. This enhancement is expected to improve the efficiency of soybean transfection.

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근적외선분광법을 이용한 밀 종자 발아 예측
Prediction of Seed Viability in Wheat (Triticum aestivum L.) Using Near-Infrared Reflectance Spectroscopy
Hee-Jong Woo, Kyung-Soon Kim, So Yeon Hong, Hyemyeong Yoon, Nayoung Ro, Gyu Taek Cho, Ho-Cheol Ko, Dong Su Yu
Korean. J. Breed. Sci. 2024;56(4):461-469.
Published online December 1, 2024
DOI: https://doi.org/10.9787/KJBS.2024.56.4.461

In this study, we aimed to develop a method for the rapid and nondestructive prediction of wheat seed viability using Near-Infrared Spectroscopy (NIRS). Thirteen wheat cultivars were used to establish and validate an NIRS calibration model. The seed samples were divided into a calibration set (n=1,360) and a validation set (n=1,000), representing a wide range of germination rates created through the accelerated aging treatment (98±2% relative humidity, 40°C, 0-10 days). Spectral data were collected within the wavelength range of 400-2,500 nm. Among the three regression models tested, the Modified Partial Least Squares (MPLS) model exhibited the best performance for predicting seed viability, achieving the highest coefficient of determination (R2=0.936) and lowest standard error of calibration (SEC=7.514). The results of this study highlight the utility of NIRS-based models for the rapid, nondestructive assessment of seed viability in wheat. Additionally, this is the first study to apply NIRS for the nondestructive evaluation of wheat seed viability, providing a substantial advancement in seed quality assessment.

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Image-based digitalization of germplasm stock holds significant promise for accelerating plant breeding and crop improvement. This technology facilitates efficient germplasm characterization, evaluation, and management through the capture and analysis of visual phenotypes. However, widespread adoption is hindered by challenges that include image quality control, data analysis complexity, and phenotypic representation limitations. This study investigated these constraints and proposed strategies to address them. By managing technical challenges, refining phenotypic data extraction, and developing robust data analysis pipelines, researchers can fully leverage image-based digitalization to enhance germplasm utilization and contribute to sustainable agriculture.

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This study reports the breeding history and main characteristics of a new chestnut (Castanea crenata Siebold & Zucc.) cultivar, ‘EePi No. 2’, developed by the National Institute of Forest Science in Korea. ‘EePi No. 2’ originated from a cross between ‘Riheiguri’ and ‘Ganne’ in 2000, and was first selected in 2007, followed by secondary selection from 2008 to 2011. From 2012 to 2015, a series of stability tests for fruit traits were conducted, confirming its distinct characteristics. The cultivar was used for plant variety protection in November 2015 (Application No. 2015-25) and was officially registered in January 2024 under Registration No. 338. ‘EePi No. 2’ is classified as a mid- to late-maturing cultivar, with a harvest period from late September to early October. The average nut weight is 30.1 g, with a soluble solid content of 12.35%, and the pellicle removal rate was 90.5%. Compared to the control cultivar ‘Daebo,’ ‘EePi No. 2’ produces larger nuts with superior pellicle removability, making it suitable for roasted chestnuts and fresh consumption. These results suggest that ‘EePi No. 2’ is a promising new cultivar that can contribute to the production of high-quality chestnuts and the expansion of consumer markets (Registration No. 338).

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논 적응성이 높고 다수성인 유채 1대 잡종 품종 ‘아름’
‘Areum’: F1 Hybrid Rapeseed with Superior Paddy Adaptation and Yield
Jaehee Jeong, Da-Hee An, Hyun-Min Cho, Kyung-Chul Cho, Young-Lok Cha, Eunyoung Oh, Ji-Bong Choi, Dong-Sung Kim, Yeong-Jun Kim, Ji-Eun Lee, Yong-Ku Kang, Kwang-Soo Kim
Korean. J. Breed. Sci. 2026;58(1):71-79.
Published online March 1, 2026
DOI: https://doi.org/10.9787/KJBS.2026.58.1.71

The F1 hybrid rapeseed (Brassica napus L.) exploits heterosis to deliver greater yield, stability, and uniformity than open-pollinated cultivars, making it suitable for large- scale cultivation. In Korea, cultivation is shifting from landscape and apiculture to expanding edible oil production and increasing the demand for paddy field-adapted hybrids. Using Mokpo-CGMS as the female and the pollen parent ‘09003-B-126-2-1-3,’ we developed the breeding line ‘Dangyo 80,’ which, after multi-environment evaluations of yield and oil quality against ‘Sunmang,’ was selected as a new cultivar ‘Areum. ’ ‘Areum’ exhibits growth habit type IV, yellow flowers, and a red seed coat. Its flowering and maturity times are comparable to ‘Sunmang. ’ Major growth indices did not differ significantly among cultivars; however, while ‘Areum’ showed slightly greater lodging susceptibility, it displayed higher resistance to Sclerotinia stem rot. Notably, in paddy fields ‘Areum’ achieved a higher plant density per square meter than ‘Sunmang’ which produced a 31% yield increase. In fatty acid composition, ‘Areum’ had higher oleic acid content (65% vs. 60%) and lower erucic acid (0.95% vs. 1.35%) than ‘Sunmang,’ indicating improved suitability for high-quality edible rapeseed oil production (Registration No. 9755).

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종자가 크고 종피가 연하며 로즈마린산 함량이 높은 종실들깨 ‘늘새미’ 육성
Development of a Perilla Cultivar ‘Nulsaemi’ with Large Seeds, A Soft Seed Coat, and High Rosmarinic Acid Content
Sang Woo Kim, Jung-In Kim, Myoung Hee Lee, SungUp Kim, Jeongeun Lee, Eunsoo Lee, Eunyoung Oh, Ki Young Kim
Korean. J. Breed. Sci. 2026;58(1):53-61.
Published online March 1, 2026
DOI: https://doi.org/10.9787/KJBS.2026.58.1.53

Perilla is a traditional oilseed crop cultivated in Korea. The ‘Nulsaemi’ cultivar was developed for oil use because of its large seed size and high functional compound content. It originated from a cross between ‘Daesil’ (IT274265), with high crude fat content and a soft seed coat, and ‘Milyang16’ (K015415), with large, brown seeds and a hard seed coat. The cross was created in 2008, and promising lines were selected through pedigree breeding up to the F6 generation. Advanced yield trials were conducted in 2016 and regional yield trials were conducted at four sites (Milyang, Suwon, Cheongju, and Iksan) from 2017 to 2019. ‘Nulsaemi’ matures around October 8, which is slightly later than the standard cultivar ‘Dayu’. It also has a 1,000-seed weight of 5.2 g, which is 1.4 g heavier than ‘Dayu’. The seed hardness was low, making it suitable for processing. The average yield was 125 kg/10a, about 3% higher than that of ‘Dayu’. The seeds contained approximately 45.1% crude fat and 63.4% alpha-linolenic acid, similar to those of the standard cultivar. However, rosmarinic acid content was significantly higher at 2,130.6 μg/g, about 18% more than ‘Dayu’. In sensory evaluation, oil extracted from ‘Nulsaemi’ showed better appearance, aroma, taste, and overall preference compared to the control, in both cold-pressed and roasted oil types. ‘Nulsaemi’ is expected to be a high-quality oilseed cultivar suitable for premium perilla oil production (Registration No. 8792).

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Developing a unique local variety that adapts well to the regional environment and consistently expresses its own characteristics provides a significant competitive advantage in the branded rice market. The aim of this study was to develop a Mid-Late Maturing Rice Cultivar with High Grain Quality adapted to the Chungnam Plain. ‘Daebang’ was developed by crossing ‘Haedamssal,’ a stable and high-yielding cultivar, with ‘Jinsumi,’ known for excellent grain appearance and eating quality, during the winter of 2013-2014. The F1 was grown in summer 2014 to produce F2 seeds, and promising lines were selected via the pedigree method. Under normal transplanting, ‘Daebang’ headed on August 19, about four days later than ‘Samkwang. ’ It had a culm length of 75 cm (9 cm shorter than ‘Samkwang’), indicating better lodging resistance. It had 84 spikelets per panicle, a 1,000-grain weight of 21.9 g, and a milled rice yield of 6.06 MT ha-1, which was numerically higher but not significantly different from that of ‘Samkwang.’ The head rice ratio was 95.9%, with a protein content of 5.4%, and glossiness value 78.5, indicating a superior grain quality. ‘Daebang’ also showed similar resistance to blast and bacterial leaf blight as ‘Samkwang.’ Therefore, ‘Daebang’ is expected to serve as a promising mid-late maturing cultivar for producing premium-branded rice in the Chungnam region because of its high grain quality, lodging tolerance, and stable yield (Registration No. 9169).

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두부 가공적성 및 기계화 적응성이 우수한 대립 다수성 콩 신품종 ‘다드림’
The Soybean Cultivar ‘Dadrim’ Exhibits a High Tofu Process Ability, Large Seeds, and High-Yield
Beom Kyu Kang, Jeong Hyun Seo, Jun Hoi Kim, Su Vin Heo, Man Soo Choi, Jee Yeon Ko, Byung Won Lee, In Youl Baek, Jae Seong Park, Choon Song Kim, Ji Ho Choo, Won Young Han, Myeong Cheol Seo, Myung Hee Lee, Nam Geol Kim, Gi Young Kim, Taek Rim Lee, Ji Ae Lee, Sol Ji No, Seung Su Lee, Young Min Jo, Jin Sil Choi, Hyun Tae Jo, Jin Young Kim
Korean. J. Breed. Sci. 2026;58(1):31-41.
Published online March 1, 2026
DOI: https://doi.org/10.9787/KJBS.2026.58.1.31

A new soybean [Glycine max (L.) Merr.] cultivar, ‘Dadrim,’ was developed by the National Institute of Crop and Food Science (NICS). ‘Dadrim’ is characterized by its superior suitability for tofu processing and high adaptability to mechanized cultivation. This cultivar ‘Milyang374’ was developed using the pedigree method from an artificial cross made in 2012 between ‘Milyang231’ and ‘Chamol.’ Its agronomic characteristics and production stability were verified through regional adaptation trials conducted over three years (2020- 2022). ‘Dadrim’ exhibits a determinate growth habit, white flowers, and spherical seeds with a yellow seed coat and yellow hilum. It is a large-seeded variety with a 100-seed weight of 30.9 g. As a late-maturing variety, it reaches maturity around October 22. Despite a relatively short plant height, it features a high first pod height of 16 cm, making it advantageous for mechanical harvesting. ‘Dadrim’ is resistant to bacterial pustule and soybean mosaic virus and possesses excellent lodging resistance. Notably, ‘Dadrim’ demonstrates a high tofu yield of 283%, which is 67% points higher than ‘Daewonkong’. The resulting tofu has a soft texture, making it suitable for soft and silken tofu. It also received high preference scores for taste and texture in sensory evaluations. ‘Dadrim’ yielded 3.37 tons/ha, an 8% increase compared to ‘Daewonkong.’ This new variety is expected to increase farm income and advance the domestic tofu industry (Registration No. 10461).

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Aralia elata (Miq.) Seem. (the Korean angelica tree) is a popular wild spring vegetable crop in East Asia. Owing to its ease of cultivation and harvesting from the second year after planting, this species is a promising forest crop for short-term income. However, the presence of prickles on the stems and shoots reduces harvesting efficiency and negatively affects product quality. This study reports the breeding and morphological characteristics of a new prickle-less cultivar, ‘Seochun’, which exhibits strong cold tolerance and early sprouting. Among the 192 native accessions collected across Korea, a superior prickle-less and cold-tolerant clone was selected and evaluated over two years (2019-2020) of field trials. The stems of ‘Seochun’ were completely smooth and prickle-less, with a larger basal diameter (40.0 mm) compared to the control (29.7 mm). The sprouting and harvesting times were approximately seven days earlier than those of the control. As an early harvest and prickle-less cultivar of A. elata, ‘Seochun’ is expected to improve both the shoot productivity and labor efficiency during harvesting and cutback operations (Research support number: 375).

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적색배 ‘중모적색1호’ 육성
Development of a Red-skinned Interspecific Hybrid Pear Cultivar, ‘Jungmojeoksaek1ho’
Haewon Jung, Keumsun Kim, Kyungho Won, EuDdeum Choi, Ung Yang, Young-Sik Cho, Seokkyu Yun
Korean. J. Breed. Sci. 2025;57(4):565-570.
Published online December 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.4.565

Red skin coloration in pears is caused by anthocyanin accumulation, which contributes to antioxidant activity and enhances consumer preference because of its attractive appearance. However, anthocyanin levels are strongly affected by light, making it difficult to maintain stable coloration under bagging practices commonly used in Korea. In this study, we developed the first red-skinned pear cultivar in Korea, ‘Jungmojeoksaek1ho’ (Pyrus pyrifolia×(P. pyrifolia×P. communis)), and examined changes in anthocyanin content according to the timing of bagging. ‘Jungmojeoksaek1ho’ originated from a cross between ‘Manpungbae’ (P. pyrifolia) and ‘Oharabeni’ (P. pyrifolia×P. communis) in 2003 and was registered as a new cultivar in 2025 (Registration No. 10597). The trees were vigorous, with a spreading growth habit. The mean fruit weight was 291.5 g, lower than that of the control cultivar ‘Wonhwang’ (P. pyrifolia), with soluble solids content of 12.8° Brix and titratable acidity of 0.31%. Anthocyanin content analysis of the peel revealed that ‘Jungmojeoksaek1ho’ maintained high levels until 80 days after full bloom (DAFB), followed by a sharp decline. Bagging at 60 or 80 DAFB reduced anthocyanin accumulation, whereas bagging after 100 DAFB maintained relatively high levels in the later stages. These results suggested that bagging after 100 DAFB was optimal for achieving stable red coloration (Registration No. 10597).

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충남지역 기후에 적합한 한지형 마늘 ‘기찬’
‘Gichan’, A Northern-type Garlic Cultivar Suitable for Chungnam Area Climate, Korea
Joongwon Lee, Jeachul Shin, Kwonseo Park, Woonseop Kim
Korean. J. Breed. Sci. 2025;57(4):537-545.
Published online December 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.4.537

A new northern-type garlic cultivar, ‘Gichan’, was developed by the Chungcheongnam-do Agricultural Research and Extension Services to improve adaptability and productivity in the Chungnam region Korea. ‘Gichan’ originated from a cross between fertile garlic lines K24 (♀) and 9209 (♂) in 2013, and a superior line was selected and tested from 2014 to 2020. Compared with the local cultivar ‘Taeanjong’, exhibited earlier emergence, clove differentiation, and harvest maturity and was thus classified as an early maturing type. Its bulbs were larger and heavier with an average yield of 1,617 kg per 10a, representing a 59% increase over ‘Taeanjong. Mineral contents such as Ca, Fe, and Zn were higher, and the alliin content was 51% greater, indicating improved functional quality. ‘Gichan’ exhibited resistance to leaf blight but was relatively susceptible to root mite damage. These results suggest that ‘Gichan’ is a high-yielding, early-maturing, and functional garlic cultivar suitable for the Chungnam climate and is expected to enhance farm income (Registration No. 9813).

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이삭이 크고 착립장률이 우수한 검정찰옥수수 ‘알찬흑찰’
‘Alchanheukchal’, A Black Waxy Corn Variety with Large Ears and Excellent Kernel Setting Rate
Hwan-Hee Bae, Young-Sam Go, Jae-Han Son, Jun-Young Ha, Jin-Seok Lee, Jung-Tae Kim, Seong-Hyu Shin, Yu-Chan Choi, Tae-Wook Jung
Korean. J. Breed. Sci. 2025;57(4):529-536.
Published online December 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.4.529

‘Alchanheukchal’ is a single-cross hybrid waxy corn cultivar developed by using the inbred line ‘KBW16’ as the seed parent and ‘KBW23’ as the pollen parent. Yield trials were conducted in 2013 and 2015 followed by regional adaptability tests at six locations over three years (2018-2020). Recognized for its superior performance, it was designated as a new variety by the NICS in 2020. ‘Alchanheukchal’ contains 32.9 μg 100 g-1 of anthocyanins, resulting in black-colored ears and exhibiting an intermediate ear shape. Its silking period was 74 days, equivalent to that of the standard variety ‘Ilmichal’. The ear length was 19.3 cm, similar to ‘Ilmichal’, while the kernel set rate was 94%, which was lower than that of ‘Ilmichal’. In terms of disease and pest resistance, including southern rust, northern corn leaf blight, and corn borer, as well as lodging resistance, ‘Alchanheukchal’ was comparable to ‘Ilmichal’. The number of ears and ear weight per 10a were 6,336 ears and 1,136 kg, respectively, which indicates a higher ear number but lower ear weight than ‘Ilmichal’. The 100-kernel weight was 27.7 g, smaller than that of ‘Ilmichal’. In sensory evaluation tests, the overall acceptability score was 4.7, which was lower than that of ‘Ilmichal’. The highest seed production efficiency was observed when the planting ratio of the seed parent to pollen parent was 2:1. However, because the silking date of the pollen parent occurred later, it was considered necessary to perform staggered sowing (Registration No. 10033).

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당도가 높고 식미가 우수한 단옥수수 ‘고당옥2호’
‘Godangok2’, A Single-cross Sweet Corn Hybrid with High Sugar Content and Superior Eating Quality
Hwan-Hee Bae, Young-Sam Go, Jae-Han Son, Jun-Young Ha, Jin-Seok Lee, Jung-Tae Kim, Seong-Hyu Shin, Yu-Chan Choi, Tae-Wook Jung
Korean. J. Breed. Sci. 2025;57(4):521-527.
Published online December 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.4.521

‘Godangok 2’, a yellow sweet corn hybrid obtained by crossing inbred KSE14 (female) and KSE23 (male) varieties harboring the sugary enhancer (se) gene, was developed by the maize breeding team at the National Institute of Crop Science (NICS), Rural Development Administration, Korea, in 2020. Days to silking of ‘Godangok 2’ (65 days) was 3 days later than ‘Danok 3’ (check hybrid, 62 days). The ear length of ‘Godangok 2’ (18.9 cm) was similar to that of ‘Danok 3’, but it exhibited a higher kernel filling rate (97%). In addition, the lodging tolerance of ‘Godangok2’ was greater than that of ‘Danok3’. The number of ears per 10a in Godangok2 (6,177) was 4% higher than that in ‘Danok 3’, whereas ear weights (1,075 kg) were 4% lower. ‘Godangok 2’ had a high sugar content of 23.4° Brix, a thin pericarp thickness of 21.4 μm, and a light 100-kernel weight of 25.4 g compared to that of the check hybrid. ‘Godangok 2’ showed the highest seed yield of 270 kg/10a at a 4 (female):1 (male) planting ratio. Because ‘Godangok 2’ carries the recessive se gene, its unique qualities may be compromised by cross-pollination with other corn varieties (e.g., sh2, bt, wx types). Therefore, to maintain sweet corn quality, corn should be grown and harvested separately in time and space (Registration No. 10059).

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리그난 함량이 높고 항산화 활성이 우수한 내탈립 참깨 ‘슈퍼하니’
High Lignan, Antioxidant-rich, and Non-shattering Sesame Cultivar ‘SuperHani’
Jeongeun Lee, SungUp Kim, Jung In Kim, Sang Woo Kim, Eunsoo Lee, Myoung Hee Lee, Eunyoung Oh, Min Young Kim
Korean. J. Breed. Sci. 2025;57(4):513-520.
Published online December 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.4.513

‘SuperHani’ is a sesame (Sesamum indicum L.) cultivar characterized by high lignan content and a non-shattering trait. It was developed in 2022 through a cross between ‘Suwon195’ and ‘YCS71’. This cultivar has a highly branched triple-capsule growth type, with 117 capsules per plant and a 1,000-seed weight of 2.0 g. Field evaluations revealed non-shattering traits, with seeds not falling even after the capsules were split open, indicating suitability for mechanical harvesting. Seed quality analyses demonstrated a total lignan content of 14.8 mg/g, with total polyphenol and flavonoid contents as well as antioxidant activities being significantly higher than those of the control. In regional adaptability trials, the average yield was 120 kg/10a, approximately 90% that of ‘Geonbaek’, but this difference was not statistically significant. These results suggest that ‘SuperHani’ is a mechanically harvestable cultivar that combines non-shattering traits with high levels of functional compounds. This has the potential to contribute to labor savings and the production of high-value sesame (Registration No.10316).

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고함량의 phytoene, phytofluene을 함유하는 토마토 신품종 ‘루비벨’ 육성
Development of Tomato (Solanum lycopersicum) Cultivar ‘Rubybell’ with High Phytoene and Phytofluene Levels
Seon Ah Son, Youngbae Kim, Eun Kim, Ki Hoon Lee, Yeo-Jin Choi, Jin Seok Kim, Jaegyun An, Yeonjeong Park, Yeweon An, Sunoh Kim
Korean. J. Breed. Sci. 2025;57(4):505-512.
Published online December 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.4.505

This study aimed to develop a novel tomato (Solanum lycopersicum L.) cultivar enriched in phytoene and phytofluene, colorless carotenoids known for their health-promoting properties. Parental lines with diverse fruit colors and morphological traits were selected and crossbred to generate three F1 hybrids: ‘Rubybell’, ‘INDIGO-G’, and ‘BLACK-B’. The phytoene and phytofluene contents of the hybrids were quantified using high-performance liquid chromatography. Among them, ‘Rubybell’ exhibited the highest concentrations of phytoene (0.105 mg/g) and total phytofluene (0.118 mg/g), representing increases of 51% and 29% compared to the maternal line APR-52-MU, and 14% and 1% compared to the paternal line CAPP1745, respectively. Compared to commercial tomato cultivars, ‘Rubybell’ exhibited 76- and 23-fold higher levels of phytoene and phytofluene, respectively. Furthermore, ‘Rubybell’ accumulated higher levels of these functional compounds when cultivated in March and October, underscoring the influence of the planting season on carotenoid biosynthesis. These findings offer valuable insights into the breeding of high-value functional tomatoes and support their potential use in health-promoting food applications (Registration number: 10514).

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To improve resistance to planthoppers and viral diseases, a new rice cultivar named ‘Drimi9ho’ was established. The F1 population, derived from a cross between ‘Cheongcheong’ and ‘Nagdong’, was subjected to anther culture. Subsequent generations were advanced in the field under high selection pressure for agronomic traits and disease resistance. Ultimately, the line ‘CNDH-AC63-2-2-2-1’ was selected through the pedigree method and was named ‘Drimi9ho’ after undergoing yield trials and local adaptability tests. ‘Drimi9ho’ has a heading date of August 13, which corresponds to 107 days after sowing, making it a mid-late maturing variety that heads 3 d later than ‘Cheongcheong’. The culm length of ‘Drimi9ho’ is 57.3 cm, which is 15 cm shorter than that of ‘Cheongcheong’. This reduced culm length contributes to improved tolerance to field lodging. In addition, ‘Drimi9ho’ exhibits acquired resistance to rice black-streaked dwarf virus through introgression from ‘Nagdong’. Compared to ‘Cheongcheong’, ‘Drimi9ho’ shows lower protein content and higher amylose content, which resulted in a higher score for cooked-rice palatability. ‘Drimi9ho’ shows improved milling performance compared to ‘Cheongcheong’. The 1,000-grain weight of ‘Drimi9ho’ is 22.12±0.8 g, which is heavier than that of ‘Cheongcheong’ (19.42±0.8 g). The milled rice yield of ‘Drimi9ho’ is 546.6±3.8 kg/10a, representing a 4.7% increase compared to ‘Cheongcheong’ (522.2±5.8 kg/10a). However, ‘Drimi9ho’ is resistant only to bacterial leaf blight race K1; thus, timely disease management is required to control other races such as K2, K3, and K3a (Registration No. 10610).

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구기순 및 잎 생산에 알맞은 4배체 구기자 ‘청순’
‘Cheongsoon’, A Tetraploid Goji Berry (Lycium chinense Mill.) Cultivar Suitable for Sprout and Leaf Production
Jeong Lee, Bohee Lee, Seonjoong Kim, Sanglim Yoon, Hyungu Choi, Juyeong Kim, Sudong Kim
Korean. J. Breed. Sci. 2025;57(4):483-491.
Published online December 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.4.483

The goji berry (Lycium chinense Mill.) line CBP17749-20 was selected from F1 hybrids of ‘CBP02261-1’ and ‘CBP11542-206’ in 2017 to establish a cultivar suitable for shoot and leaf production. Preliminary and advanced yield trials were conducted in 2019 and 2020 to investigate yield characteristics, and the selected line was named ‘Gugisoon 2.’ After regional adaptability tests from 2021 to 2022, it was officially registered as the cultivar ‘Cheongsoon.’ The cultivar Unk ‘Cheongsoon’ is characterized by vigorous growth, strong sprouting ability, and an upright growth habit. Its leaves are broadly oval with a higher chlorophyll content than those of the control cultivar ‘Gugisoon 1’ and both leaves and stems are thicker and heavier. It also exhibits excellent resistance to gall mites (Eriophyes spp.), which cause purplish galls on leaf surfaces. Compared to ‘Gugisoon 1,’ ‘Cheongsoon’ has a higher rutin content and a lower quercetin content, and its shoot production index is 224% greater. This cultivar is highly suitable for shoot and leaf production and can be grown in open fields; however, greenhouse cultivation produces tender shoots (Registration No. 10170).

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Research Articles
가지 길이가 짧고 기름 함량이 높은 고올레산 땅콩 ‘케이올2호’
‘K-Ol2’: A Peanut Variety with Short-stem, High-oil, and High-oleic Acid Content
Eunyoung Oh, Gyeong-Dan Yu, Da-Hee An, Jaehee Jeong, Hyun-Min Jo, Young-Lok Cha, Sungup Kim, Jung In Kim, Min Young Kim, Sang Woo Kim, Jeongeun Lee, Eunsoo Lee, Myoung-Hee Lee
Korean. J. Breed. Sci. 2025;57(4):465-473.
Published online December 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.4.465

‘K-Ol2’ is a high-oil, high-oleic acid peanut (Arachis hypogaea L.) variety developed by the National Institute of Crop and Food Science and released in 2022. This variety was derived through pedigree selection from a single cross between the variety ‘Jaseon’ and the high-oleate elite line ‘YG377’. High-oleate lines were selected in the F3 generation using Near-Infrared Spectrophotometry and gas chromatography. ‘K-Ol2’ exhibits a relatively short plant height with main stem and branch lengths of 29 cm and 36 cm, respectively, which are less than those of the check variety ‘Akwang’. The seeds had a brown testa and an elliptical kernel shape, with a 100-seed weight of 87 g and an average of 54 mature pods per plant. Seed composition analysis revealed an average protein content of 29.3% and a high crude fat content of 54.5%. The oleic acid (C18:1) content reached 82.8%, with an oleic to linoleic acid ratio of 28.5:1, significantly higher than that of ‘Akwang’ (2.2:1). In oxidative stability evaluations under accelerated oxidizing conditions, ‘K-Ol2’ exhibited an extended induction time to reach maximum conductivity increase, indicating improved resistance to lipid oxidation. Regional yield trials conducted from 2020 to 2022 demonstrated that ‘K-Ol2’ produced an average yield of 4.60 MT⋅ha-1, representing a 10% increase over ‘Akwang’. Due to its high oil content and improved oxidative stability, ‘K-Ol2’ is expected to play a valuable role in the peanut processing industry (Registration No. 10358).

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Pears (Pyrus spp.) are economically important fruit trees grown extensively world-wide. To identify QTLs related to key agronomically important fruit traits, we conducted QTL analysis using an interspecific 178 F1 populations derived from a cross between ‘Manpungbae’ and ‘Oharabeni’. Phenotyping data, including fruit weight, fruit length and diameter, fruit shape index (FSI), soluble sugar content, fruit firmness (FF), and titratable acidity (TA) were investigated from 2016 to 2018. Using the previously constructed genetic linkage map together with the three-year phenotypic dataset, we detected 14 significant QTLs associated with the control of fruit weight, fruit length and diameter, FSI, FF, and TA on linkage groups 3, 10, 11, 12, and 13. These findings provide a useful resource for advancing genetic and genomic studies in pears and will aid breeding efforts to improve major fruit traits.

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가뭄내성 GM콩의 영양성분 동등성 비교
Comparative Analysis of Compositional Equivalence in Drought-Tolerant Genetically Modified Soybeans
Ha-Jung Kang, Hyoun-Min Park, Sung-Dug Oh, Ye-Jin Jang, Jong-Chan Park, Seon-Woo Oh, Sang-Gu Lee, Soo-Yun Park, An-Cheol Chang
Korean. J. Breed. Sci. 2025;57(4):445-453.
Published online December 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.4.445

Global climate change has intensified droughts and other extreme weather conditions, leading to serious declines in agricultural productivity. Genetically modified (GM) soybeans with drought tolerance have been developed to enhance crop resilience. Prior to commercialization, GM crops must undergo compositional equivalence assessments to confirm that no unintended compositional differences exist compared to their non-GM counterparts. In this study, we evaluated the nutritional and compositional equivalence of two drought-tolerant GM soybean lines (DIAT7 and DIAT15) compared to their non-GM parental line (Williams 82) and three reference cultivars (Kwangan, U13625, and U14511). Soybeans were cultivated under identical field conditions, and proximate components, amino acids, fatty acids, minerals, and antinutritional factors were analyzed using standard methods. Although several analytes exhibited statistically significant differences (p<0.05), all compositional values for DIAT7 and DIAT15 were within the natural variation ranges of the reference cultivated with GM, OECD (2012), and the AFSI Crop Composition Database (v10.1). The GM lines showed protein (36-37%), lipid (19-21%), and total dietary fiber (24-27%) contents similar to those of the reference cultivars. Amino acid profiles were dominated by glutamic acid and aspartic acid, whereas linoleic acid (42-51%) and oleic acid (29-36%) were the major fatty acids, consistent with conventional soybeans. The levels of minerals and antinutrients, including raffinose, stachyose, and phytic acid, were also comparable to the reference ranges. These findings demonstrate that the drought-tolerant GM soybeans DIAT7 and DIAT15 are compositionally and nutritionally equivalent to non-GM soybeans, supporting their substantial equivalence and providing a scientific basis for food and environmental safety evaluation.

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The composition of high-molecular-weight-glutenin subunits (HMW-GS) is a key determinant of wheat baking properties. These subunits are encoded by the GLU-A1, GLU-B1, and GLU-D1 loci on the long arm of chromosome 1 and consist of x- and y-type subunits. Allelic variations in composition are a major factor influencing bakery quality. Unlike sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) or ultra-performance liquid chromatography (UPLC), which often fail to resolve closely related allelic variants, PCR-based markers allow for clear and definitive discrimination at the DNA level. Building on the results of a previous study that determined the GLU-B1 allele composition, we aimed to confirm—through the use of PCR markers—the allele compositions of GLU-A1 and GLU-D1 in 44 domestic wheat varieties. The results showed that “Jonong” and “Sinmichal1” contained the Glu-A1b (A1x2*) allele rather than Glu-A1a (A1x1) or Glu-A1c (A1x-null). Additionally, “Jonong” and “Sinmichal1” exhibited the allelic composition Glu-D1a (D1x2+D1y12), rather than Glu-D1d (D1x5+D1y10) or Glu-D1f (D1x2.2+D1y12). These results were compared with those obtained by SDS-PAGE and UPLC. The PCR-based markers used to identify GLU-A1 and GLU-D1 alleles in this study will be valuable for determining the allelic composition at the GLU-A1 and GLU-D1 loci in domestic wheat varieties. Furthermore, the re-evaluated genetic composition is expected to improve the precision of assessments related to the baking quality of domestic wheat.

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Mapping the Genetic Architecture of Tofu Quality Traits in Korean Soybean
콩에서 두부특성 연관 양적형질유전자좌(QTLs) 탐색
D.S. Kishor, Jiyoung Jung, Ji-Min Kim, Taeklim Lee, Seokjin Roh, Yoo Jeong Choi, Maeng Seon Kim, Changmi Im, Sungtaeg Kang
Korean. J. Breed. Sci. 2025;57(4):419-432.
Published online December 1, 2025
DOI: https://doi.org/10.9787/KJBS.2025.57.4.419

Tofu, a widely consumed soy-based product, has seen increasing consumer demand, driven by health and dietary trends. Enhancing the processing suitability of soybeans requires analysis of the genetics associated with tofu quality traits. In this study, five key traits, namely water absorption (WA%), Brix of soymilk (°BS), soymilk yield (YM%), tofu residue (TR%), and tofu yield (YT%), were evaluated in two recombinant inbred line (RIL) populations: 81 lines of “Daepung”דSaedanbaek” and 122 lines of “Daepung”דGalchae”. Most traits exhibited normal distributions and transgressive segregation. Trait correlations revealed strong associations between WA and TR, and between YM and YT, with population-specific variation. Quantitative trait locus (QTL) mapping identified eight loci in each population linked to WA, °BS, YM, TR, and YT across multiple chromosomes. Notably, co-localized QTL were detected for WA% and TR% (chromosomes 1 and 6) and for WA%, YM%, °Brix, and TR% (chromosome 8), indicating pleiotropic regulation. Candidate genes included Glyma.06g173500, associated with β-glucan biosynthesis, and Glyma.03g132700, encoding β-glucanase, both implicated in seed coat permeability and tofu yield. These results provide novel genetic insights into seed coat properties and processing quality, and the identified QTLs and candidate genes represent valuable targets for marker-assisted selection in soybean breeding programs optimized for tofu production in Korea.

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