Chrysanthemum (Dendranthema grandiflourm Kitamura) is a member of the Asteraceae and one of the important horticultural crops. The objective of this study was to construct a DNA profile database for identification of chrysanthemum varieties using simple sequence repeat (SSR) markers. In order to select SSR markers for the variety identification, we screened 587 SSR primers using 20 varieties. Among them, 27 SSR markers showed polymorphism. We finally selected 14 SSR markers showing peak clearance, high polymorphism and reproducibility in 20 varieties. In conclusion, DNA profile database for 147 chrysanthemum varieties were constructed by 14 SSR markers. A total of 79 SSR alleles were detected and three to ten alleles were detected with an average of 5.6 alleles per locus. The polymorphism information content value ranged 0.287 ~ 0.785 with an average of 0.598. Genetic relationship revealed that genetic distance of 147 varieties ranged from 0.44 to 1.00. The 143 varieties among 147 varieties were distinguished by 14 SSR markers but the 2 varieties developed by mutation breeding and natural variation were not distinguished from original varieties. These constructed SSR profile database will be useful for the selection of similar varieties for candidate variety and for solving problem relating to seed dispute and infringement of plant breeder’s right.

Citations

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• Evaluation of Genetic Diversity and Identification of Cultivars in Spray-Type Chrysanthemum Based on SSR Markers
  Manjulatha Mekapogu, So-Hyeon Lim, Youn-Jung Choi, Su-Young Lee, Jae-A Jung
  Genes.2025; 16(1): 81.     CrossRef
• Assessment of Genetic Diversity in the White-Colored Variants of Spray-Type Chrysanthemum Cultivars Using SSR Markers
  Manjulatha Mekapogu, Hyun-Young Song, So-Hyeon Lim, Jae-A Jung
  Horticulturae.2023; 9(7): 798.     CrossRef
• Identification of standard type cultivars in Chrysanthemum (Dendranthema grandiflorum) using SSR markers
  Manjulatha Mekapogu, Oh Keun Kwon, Do Yoon Hyun, Kyung Jun Lee, Myung Suk Ahn, Jong Taek Park, Jae A Jung
  Horticulture, Environment, and Biotechnology.2020; 61(1): 153.     CrossRef
• Development of EST-SSR Markers for Cultivar Determination and Genetic Diversity Studies of Commercial Chrysanthemums in the Korean Floral Market
  Jeong-Yun Han, Jung-Bun Kim, Ho-Jin Lee, Chang Pyo Hong, Ha-Seung Pak, Tae-Sung Kim
  Korean Journal of Breeding Science.2019; 51(3): 201.     CrossRef

This study was conducted to construct a DNA marker database for 38 plum varieties collected in Korea using simple sequence repeat (SSR) markers. A set of 61 SSR primer pairs was tested to select polymorphic SSR markers between 8 varieties. Among the 61 primer pairs, 21 showed polymorphism, reproducibility and easy scoring. The genetic relationship between the 21 SSR markers and 38 varieties was analyzed. A total of 210 polymorphic amplified fragments were obtained with the 21 SSR markers. Three to seventeen SSR alleles were detected for each locus, with an average of 10.0 alleles per locus. Average polymorphism information content (PIC) was 0.758, with a range from 0.549 to 0.870. A total of 210 SSR marker loci were used to calculate Jaccard’s distance coefficients for cluster analysis by an unweighted pair-group method with arithmetical average (UPGMA). The genetic distance ranged from 0.06 to 1.00 in 38 varieties. Out of 38 plum varieties, 32 were identified using the 21 SSR markers. Therefore, these SSR markers may be employed to complement distinctness, uniformity, and stability (DUS) tests or as potential tools to solve seed disputes regarding plums.

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• Assessment of Genetic Diversity and Interspecific Relationships of the Genus Viburnum Inferred from Start Codon Targeted (SCoT) Polymorphism Markers
  Choi I-Jin, Park Hey-Min, Kim Hyun-Kyung
  Ecology and Diversity.2026; 3(1): 10002.     CrossRef