Chrysanthemum (Dendranthema grandiflourm Kitamura) is a member of the Asteraceae and one of the important horticultural crops. The objective of this study was to construct a DNA profile database for identification of chrysanthemum varieties using simple sequence repeat (SSR) markers. In order to select SSR markers for the variety identification, we screened 587 SSR primers using 20 varieties. Among them, 27 SSR markers showed polymorphism. We finally selected 14 SSR markers showing peak clearance, high polymorphism and reproducibility in 20 varieties. In conclusion, DNA profile database for 147 chrysanthemum varieties were constructed by 14 SSR markers. A total of 79 SSR alleles were detected and three to ten alleles were detected with an average of 5.6 alleles per locus. The polymorphism information content value ranged 0.287 ~ 0.785 with an average of 0.598. Genetic relationship revealed that genetic distance of 147 varieties ranged from 0.44 to 1.00. The 143 varieties among 147 varieties were distinguished by 14 SSR markers but the 2 varieties developed by mutation breeding and natural variation were not distinguished from original varieties. These constructed SSR profile database will be useful for the selection of similar varieties for candidate variety and for solving problem relating to seed dispute and infringement of plant breeder’s right.

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