Brassica juncea, a member of the Brassicaceae family commonly referred to as mustard, is an allotetraploid (AABB, 2n=36) resulting from interspecific hybridization between Brassica rapa (AA, 2n=20) and Brassica nigra (BB, 2n=16). In this study, microspores were cultured using F₁ hybrids between two selected B. juncea double haploid (DH) lines with high anthocyanin and glucosinolate contents in the maternal versus leafy paternity. The results of the microspore culture showed that the total number of cultured buds, obtained embryos, and embryos per bud were 2,010, 15,526, and 7.62, respectively. Colchicine was used to induce the amphidiploids (AABB). We found that 149 of the 218 regenerated plants (68.3%) were amphidoploid. Among them, 117 individuals underwent ripening and seed harvesting and were used for subsequent phenotype analysis. Leaf color, length, and other agricultural traits exhibited various patterns owing to the recombination of the parental allele. The glucosinolate (GSL) content ranged from a maximum of 29.27 μmol/g dry weight to a minimum of 6.39 μmol/g dry weight, exceeding the range of parental value. The composition of GSL was mainly aliphatic, with sinigrin accounting for approximately 89% of the total aliphatic GSL content. These DH lines and their respective unique phenotypic traits are crucial for crop breeding and are valuable resources for advancing functional genomics and developing molecular markers in B. juncea.

A new rapeseed (Brassica napus L.) F₁ hybrid ‘Haean’ was crossed between ‘Mokpo-CGMS’, male sterile line and ‘8516-B-5-6-1-2’, restore line for the adaptation of paddy land culture with early maturing, disease resistant and lodging tolerance in 2008. Yield trials were conducted from 2009 to 2010 in Muan, and regional adaptation trials were examined from 2011 to 2013 in four regions. ‘Haean’ has dark green, cleft and parted leaf, light yellowish flower, and black seed color. The maturation date of ‘Haean’ is 4^th June, 4 days earlier than ‘Sunmang’. In the regional adaptation trials, average yield of ‘Haean’ was 272 kg/10a, which was 6% higher than ‘Sunmang’. Seed oil content of ‘Haean’ was 44.2%. In fatty acid composition, oleic acid content is 69.8%, which is 2.5% higher than ‘Sunmang’. Total glucosinolates content was 1.84 mg/g. ‘Haean’ has characteristics of disease resistant and lodging tolerance, this variety can be cultured at paddy lands in southwestern area including Jeonnam, Jeonbuk and Kyongnam provinces and Cheju island of Korea.