In this study, we aimed to develop a method for the rapid and nondestructive prediction of wheat seed viability using Near-Infrared Spectroscopy (NIRS). Thirteen wheat cultivars were used to establish and validate an NIRS calibration model. The seed samples were divided into a calibration set (n=1,360) and a validation set (n=1,000), representing a wide range of germination rates created through the accelerated aging treatment (98±2% relative humidity, 40°C, 0-10 days). Spectral data were collected within the wavelength range of 400-2,500 nm. Among the three regression models tested, the Modified Partial Least Squares (MPLS) model exhibited the best performance for predicting seed viability, achieving the highest coefficient of determination (R²=0.936) and lowest standard error of calibration (SEC=7.514). The results of this study highlight the utility of NIRS-based models for the rapid, nondestructive assessment of seed viability in wheat. Additionally, this is the first study to apply NIRS for the nondestructive evaluation of wheat seed viability, providing a substantial advancement in seed quality assessment.

Identification of Fusarium oxysporum f. sp. raphani and investigation on fusarium wilt development by isolates and inoculation methods were conducted to establish a screening method for fusarium wilt-resistance in radish (Raphanus sativus L.) germplasm. Pathogenicity of F. oxysporum f. sp. raphani isolate, Heonggye-1 and Heonggye-2, to radish plants was confirmed by seedling test. Radish seedlings were inoculated by root-dipping and soil-drenching with or without root-wounding. For Heonggye-1 isolate, mean disease indexes were 4.13 and 3.91 by root-dipping and soil-drenching with root-wounding, respectively, but those were 1.87 and 1.88 without root-wounding. For Heonggye-2 isolate, mean disease indexes were 3.83 to 4.37 regardless of inoculation methods. Two-hundred sixty accessions of radish germplasm collected from 9 countries of Asia and Europe were evaluated for fusarium wilt-resistance by soil-drenching with root-wounding with Heonggye-2 isolate. Fifty-four resistant accessions with higher than 70% of the percentage of resistant seedlings in accession (P_R) and lower than 20% of the percentage of susceptible seedlings in accession (PS) was found. Eighteen susceptible accessions with lower than 20% of P_R and higher than 50% of PS were selected. These accessions could be used as breeding and research materials after re-evaluation of disease-resistance and characterization of agronomical traits.