In this study, we investigated the fruit and vine characteristics of hypo- and hyper-tetraploid grapes from which a chromosome was added or deleted. The aim of this study was to evaluate the possibility of developing seedless grapes by utilizing the characteristics of hypo- and hyper-tetraploid grapes. Both the hypo- and hyper-tetraploid grapes showed stable vine growth characteristics unlike other aneuploid plants, which displayed abnormal leaf morphology and unstable vine growth patterns, indicating that hypo- and hyper-tetraploid grapes can be used for commercial purpose. The hypo-tetraploid grapes produced 100% seedless fruits, with large berries ranging from 8.5 to 10.5 g, when the grapes were applied with 100 ppm gibberillic acid during their full bloom period. Although the fruits of the hyper-tetraploid grape were larger than those of the hypo-tetraploid grapes, it produced a lower percentage of seedless fruits. We conclude that the characteristics of hypo-tetraploid grapes may be exploited to develop seedless grapes with large berries.