Plant regeneration protocols via adventitious shoot organogenesis from leaf segments of Chrysanthemum morifolium ‘Baekma’ were developed. The effects of plant growth regulators (BA, NAA, IBA, IAA and 2,4-D) and AgNO₃ were tested to figure out the optimal condition for shoot bud induction and shoot formation from leaf explants. On the combination treatment of plant growth regulators and AgNO₃, bud induction was obtained but shoot formation was not. Therefore, two-stage treatment of leaf explants was subsequently experimented for the respective improvement of adventitious bud induction and shoot formation. When leaf explants were cultured on bud induction medium (MS medium supplemented with 0.2 mg/L BA, 1.0 mg/L IAA and 1.0 mg/L 2,4-D) for 4 weeks in darkness and were transferred to shoot formation medium (MS medium supplemented with 1.0 mg/L BA, 1.5 mg/L IBA and 5.0 mg/L AgNO₃) for 4 weeks under the 16/8h photoperiod condition, shoot formation efficiency reached up to 50.0% and 1.6 shoots per explant were acquired. At 12 weeks after culture, the regenerated shoots were elongated and rooted on MS medium. The plantlets were acclimatized successfully and the regenerated plants exhibited normal phenotypes.

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