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"Ju-Kon Kim"

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"Ju-Kon Kim"

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을 이용한 제초제 저항성 옥수수 형질전환체 생산
Production of Transgenic Maize Plants with Herbicide Resistance Through Agrobacterium-mediated Transformation
Joon Ki Hong, Gang-Seob Lee, Ki Jin Park, Ju-Kon Kim, Hee Jeung Jang, Eun Jung Suh, Kyung-Hwan Kim, Yeon-Hee Lee
Korean. J. Breed. Sci. 2019;51(4):290-297.   Published online December 1, 2019
DOI: https://doi.org/10.9787/KJBS.2019.51.4.290

Maize is the most important grain crop in the world. Genetic engineering technology has been used to enhance its various agronomical traits. The transformation of maize is a crucial step in the application of gene technologies to improve maize. The choice of genotype and explant material influences the transformation efficiency and the production of stable transgenic plants. Immature embryos of Hi IIA were infected with Agrobacterium tumefaciens LBA4404 including superbinary vectors (bar and GUS or GFP genes). The transformation efficiency was based on transgenic calli induction from immature embryos on the selection medium with 3 mg/L bialaphos. The transformation efficiency varied from 1.01 to 2.74%. The integration and expression of bar, GUS, and GFP genes were confirmed in T0 and T1 generations of transgenic plants using genomic PCR and the bar strip test. In addition, herbicide resistance in T1 transgenic plants was observed when leaves and whole plants were treated with Basta. These results suggest that the successful Agrobacterium-mediated transformation of Hi IIA will improve further opportunities for functional genomic and genome editing studies in maize.

Citations

Citations to this article as recorded by  
  • Impact of genetically modified herbicide-resistant maize on rhizosphere bacterial communities
    Ye-Jin Jang, Sung-Dug Oh, Joon Ki Hong, Jong-Chan Park, Seong-Kon Lee, Ancheol Chang, Doh-Won Yun, Bumkyu Lee
    GM Crops & Food.2025; 16(1): 186.     CrossRef
  • Editing ZmWUS1 Promoter Using CRISPR/Cas9 and Improving Maize Transformation
    Da Eun Kim, Yu Mi Kang, Jum-Soon Kang, Byoung Il Je
    Journal of Environmental Science International.2025; 34(1): 25.     CrossRef
  • Overexpressing OsPYL/RCAR7 Improves Drought Tolerance of Maize Seedlings by Reducing Stomatal Conductance
    Joon Ki Hong, Yeon-Hee Lee, Beom-Gi Kim, Gang Seob Lee, Hee Jeung Jang, Giha Song, Eun Jung Suh, Sang Ryeol Park
    Agriculture.2022; 12(12): 2140.     CrossRef
  • Current research on global GM crops and approval in Korea
    Kijong Lee, Seon-Kyeong Lee, Soyoung Park, Sung-Dug Oh, Bum-Soo Hahn, Vimalraj Mani
    Journal of the Korean Society of International Agricultue.2020; 32(4): 396.     CrossRef
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유전자교정 농작물의 실용화 현황 및 전망
Gene-Edited Crops: Present Status and their Future
Soo-Chul Park, Young-Hee Joung, Kyung-Min Kim, Ju-Kon Kim, Hee-Jong Koh
Korean. J. Breed. Sci. 2019;51(3):175-183.   Published online September 1, 2019
DOI: https://doi.org/10.9787/KJBS.2019.51.3.175

Recently, several attempts have been undertaken to develop breeding technologies by combining new biotechnologies. Gene-editing technology is currently one of the most interesting areas. The plant breeding methods using this technique have the advantage of greatly improved accuracy and efficiency of the plant genetic correction compared with conventional breeding methods, which has raised expectations for the useful application of this technology as a cutting-edge breeding technology. Although not all countries around the world currently have established appropriate regulation policies on crops developed with gene-editing technology, the number of cases in which GMO regulations are not applied on a case-by-case basis according to the scientific background is growing. However, Korea has not yet established policies on which criteria should be applied to crops generated from the application of gene-editing technology. As the number of cases of crop development and commercialization using gene-editing technology is expected to increase in the near future, it will be necessary to prepare reasonable policies to support developers and seed industries in Korea to ensure harmonization with international regulatory policy trends.

Citations

Citations to this article as recorded by  
  • CRISPR revolution in plant genetics: promises, challenges and future prospects
    Ashwani Kumar, Yuan-Yeu Yau
    Vegetos.2026;[Epub]     CrossRef
  • Latest Research Trends in Reducing the Immunogenicity of Wheat
    Sora Lee, Jong-Yeol Lee, Sichul Lee, Jae-Ryeong Sim, Sewon Kim, Woo-Suk Cho
    Korean Journal of Breeding Science.2025; 57(2): 65.     CrossRef
  • Current Trends in Wheat Breeding Strategies for Developing Domestic Wheat Cultivars in Korea
    Hajeong Kang, Hyoun-Min Park, San-Gu Lee, Eun-Ha Kim, Muhammad Imran, Hanyoung Choi, Myeong-Ji Kim, Seonwoo Oh
    Korean Journal of Breeding Science.2024; 56(4): 491.     CrossRef
  • Development of virus-induced genome editing methods in Solanaceous crops
    Seo-Young Lee, Bomi Kang, Jelli Venkatesh, Joung-Ho Lee, Seyoung Lee, Jung-Min Kim, Seungki Back, Jin-Kyung Kwon, Byoung-Cheorl Kang
    Horticulture Research.2024;[Epub]     CrossRef
  • Functional Characterization of PagMYB148 in Salt Tolerance Response and Gene Expression Analysis under Abiotic Stress Conditions in Hybrid Poplar
    Su Jin Park, Hyun-A Jang, Hyoshin Lee, Hyunmo Choi
    Forests.2024; 15(8): 1344.     CrossRef
  • Enhancing the quality of staple food crops through CRISPR/Cas-mediated site-directed mutagenesis
    Olawale Samuel Adeyinka, Bushra Tabassum, Brice Landry Koloko, Ifedayo Victor Ogungbe
    Planta.2023;[Epub]     CrossRef
  • Processes for regulating genetically modified and gene edited plants
    John R Caradus
    GM Crops & Food.2023; 14(1): 1.     CrossRef
  • Safety management of living modified plants: A review
    Bumkyu Lee
    Journal of Plant Biotechnology.2022; 49(3): 163.     CrossRef
  • Research Advances in Improving Disease Resistance and Abiotic Stress Tolerance Using CRISPR/Cas9 Gene Editing in Rice
    Yurim Kim, Sieun Choi, In Sun Yoon, Song Lim Kim, Youngjun Mo
    Korean Journal of Breeding Science.2022; 54(4): 331.     CrossRef
  • TALEN-mediated bar-knockout Rice Production and Transcriptome Profiling
    Yang Qin, Tae-Sung Park, Youn Sung Cho, Myung-Ho Lim
    Plant Breeding and Biotechnology.2021; 9(1): 32.     CrossRef
  • Global Patent Trends in New Breeding Technology for Crop Improvement
    Serry Koh, Youri Choi, Joo Young Lee, Jiyoung Jang, Kyuwhan Choi
    Korean Journal of Breeding Science.2021; 53(4): 337.     CrossRef
  • Genome editing of hybrid poplar (Populus alba × P. glandulosa) protoplasts using Cas9/gRNA ribonucleoprotein
    Su Jin Park, Young-Im Choi, Hyun A Jang, Sang-Gyu Kim, Hyunmo Choi, Beum-Chang Kang, Hyoshin Lee, Eun-Kyung Bae
    Journal of Plant Biotechnology.2021; 48(1): 34.     CrossRef
  • History and Results of Rice Breeding in Korea
    Young-Chan Cho, Man-Kee Baek, Hyun-Su Park, Jun-Hyun Cho, Eok-Keun Ahn, Jung-Pil Suh, Ji-Ung Jeung, Jong-Hee Lee, Yong-Jae Won, Yoo-Chun Song, Eung-Gi Jeong, Bo-Kyeong Kim, Jeom-Ho Lee
    Korean Journal of Breeding Science.2020; 52(S): 58.     CrossRef
  • Improving Nutritional and Functional Quality by Genome Editing of Crops: Status and Perspectives
    Hyung-Keun Ku, Sun-Hwa Ha
    Frontiers in Plant Science.2020;[Epub]     CrossRef
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