Soybean (Glycine max (L.) Merr.) is one of the most important crops with economic value as a source of protein and vegetable oil for human food and animal feed. In recent years, rapidly developed genome editing techniques have shown widespread application prospects for gene function studies and for improving important agronomic traits in many crops. Therefore, it is important to establish a highly efficient method for protoplast isolation and transient expression systems in soybeans. In this study, we established an efficient method for protoplast isolation and its application to transient gene expression in Korean soybean cultivars. The protoplasts were isolated from leaves, epicotyls, hypocotyls, cotyledons, and etiolated hypocotyls using various combinations of enzyme mixtures. We found that high-quality and large amounts of protoplasts were isolated from the etiolated hypocotyls when incubated for 8 h under conditions of 0.5% cellulase, 0.5% pectinase, and 1% viscozyme. In addition, we observed a high transfection efficiency of green fluorescent protein using etiolated hypocotyl protoplasts. Taken together, our protoplast isolation and transfection method is highly efficient and can be used for gene function and molecular analysis to better understand the biological and physiological processes in soybean.

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• An Efficient Protoplast Isolation Method Using Hypocotyl in Soybean (Glycine max)
  Jaehwan Kim, Yeong Yeop Jeong, Hyunwoo Park, Pil Joon Seo, Kyung Do Kim
  Korean Journal of Breeding Science.2025; 57(1): 1.     CrossRef
• Treatment with soybean lecithin-derived α-GPC (SHCog™) improves scopolamine-induced cognitive declines in mice via regulating cholinergic neurotransmission and enhancing neural plasticity in the hippocampus
  Yulong Zheng, SoEun An, Ga-Yeon Kim, Geum Duck Park, Byong Ho Yoo, Ki Nam Kim, Tae-Kyeong Lee
  Tissue and Cell.2025; 93: 102705.     CrossRef
• Comparative untargeted metabolomic analysis of Korean soybean four varieties (Glycine max (L.) Merr.) based on liquid chromatography mass spectrometry
  Eun-Ha Kim, Soo-Yun Park, Sang-Gu Lee, Hyoun-Min Park, Oh Suk Yu, Yun-Young Kang, Myeong Ji Kim, Jung-Won Jung, Seon-Woo Oh
  Journal of Applied Biological Chemistry.2022; 65(4): 439.     CrossRef

Amphidiploid Brassica juncea (AABB, 2n=36) contains the synthesized genome of the diploid ancestors of Brassica rapa (AA, 2n=20) and Brassica nigra (BB, 2n=16), proven the ‘triangle of U’ model. Varieties of the B. juncea include vegetables, oilseed crops, and medicinal plants in South Asia, China, and other regions. ‘Dolsangat’, one of the cultivars of B. juncea is widely used as the main ingredient for ‘KatKimchi’, a kind of Korean traditional food Kimchi. To develop an efficient polyploidization protocol of B. juncea, we used twenty accessions. Among them, we could induce the amphidiploid plants with 0.23% in natural. A successful of polyploidization, it is essential of chromosome doubling regent treatment of B. juncea. At first, we tried to colchicine treatment in the embryo stage and it was very harmful to the embryo and could get few plants. The second, we made the regeneration plants from embryo to rooting phase and shocked them in 0.34% colchicine contained distilled water. We could induce amphidiploid plants with a success rate of 63.4%. Also, we surveyed glucosinolate content and JB1, Alsami, and JD6 showed high total contents. These plants will use for genetic materials for breeding, genetic and molecular studies.

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• Polyploid induction of Luculia pinceana Hook. by aseptic germination combined with chemical agents
  Lele Wang, Yefang Li, Xiaoyu Lu, Shifeng Li, Jie Song, Wenling Guan
  European Journal of Horticultural Science.2024;[Epub]     CrossRef
• Producing a Double Haploid Population Using Microspore Culture and Phenotypic Analysis in Brassica juncea
  Hyun Woo Park, Myung-Hee Kim, Mi-Sun Moon, Yoon Suk Ku, Na Hyeong Lee, Jung Sun Kim
  Korean Journal of Breeding Science.2024; 56(3): 193.     CrossRef
• Production of doubled haploid population derived from the microspore culture of rapeseed (Brassica napus L.) F1 generation and analysis of fatty acid composition
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  Journal of Plant Biotechnology.2022; 49(1): 74.     CrossRef
• In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment
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  PeerJ.2021; 9: e12399.     CrossRef

Salt stress is a significant factor limiting growth and productivity in crops. However, little is known about the response and resistance mechanism to salt stress in maize. The objective of this research was to develop an enhanced salt-tolerant silage maize by mutagenesis with gamma radiation. To generate gamma radiation-induced salt-tolerant silage maize, we irradiated a KS140 inbred line with 100 Gy gamma rays. Salt tolerance was determined by evaluating plant growth, morphological changes, and gene expression under NaCl stress. We screened 10 salt-tolerant maize inbred lines from 2,248 M₂ mutant populations and selected a line showing better growth under salt stress conditions. The selected 140RS516 mutant exhibited improved seed germination and plant growth when compared with the wild-type under salt stress conditions. Enhanced salt tolerance of the 140RS516 mutant was attributed to higher stomatal conductance and proline content. Using whole-genome re-sequencing analysis, a total of 328 single nucleotide polymorphisms and insertions or deletions were identified in the 140RS516 mutant. We found that the expression of the genes involved in salt stress tolerance, ABP9, CIPK21, and CIPK31, was increased by salt stress in the 140RS516 mutant. Our results suggest that the 140RS516 mutant induced by gamma rays could be a good material for developing cultivars with salt tolerance in maize.

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• Calcineurin B‐like interacting protein kinase 31 confers resistance to sheath blight via modulation of ROS homeostasis in rice
  Huan Chen, Qiujun Lin, Zhuo Li, Jin Chu, Hai Dong, Qiong Mei, Yuanhu Xuan
  Molecular Plant Pathology.2023; 24(3): 221.     CrossRef
• Comparison and Characterization of Phenotypic and Genomic Mutations Induced by a Carbon-Ion Beam and Gamma-ray Irradiation in Soybean (Glycine max (L.) Merr.)
  Zhuo Feng, Yan Du, Jingmin Chen, Xia Chen, Weibin Ren, Lulu Wang, Libin Zhou
  International Journal of Molecular Sciences.2023; 24(10): 8825.     CrossRef
• Frequency and Spectrum of Mutations Induced by Gamma Rays Revealed by Phenotype Screening and Whole-Genome Re-Sequencing in Arabidopsis thaliana
  Yan Du, Zhuo Feng, Jie Wang, Wenjie Jin, Zhuanzi Wang, Tao Guo, Yuze Chen, Hui Feng, Lixia Yu, Wenjian Li, Libin Zhou
  International Journal of Molecular Sciences.2022; 23(2): 654.     CrossRef

High-density genetic linkage mapping is critical for undertaking marker-assisted selection and confirming quantitative trait loci, as well as helping to build pseudomolecules of genomes. We constructed a genetic map using 94 F₁ populations generated from the interspecific cross between Korean cultivar “Wonwhang” (Pyrus pyrifolia, NCBI BioSample SAMN05196235) and European cultivar “Bartlett” (Pyrus communis). We designed a total of 24,267 SSR markers based on the genome sequences of “Wonwhang” for this. To select the markers that are linked to the traits important in pear breeding programs, SSR-containing genomic sequences were subjected to nucleotide sequence homology searches, which resulted in 510 SSR markers with high similarity to genes encoding proteins with putative functions such as transcription factors, resistance proteins, flowering time, and regulatory genes. Of these, 70 markers showed polymorphisms in parents and segregating populations and were used to construct a genetic linkage map, together with the unpublished 579 SNPs obtained from genotyping by sequencing analysis. The genetic linkage map covered 3,784.2 cM and the average distance between adjacent markers was 5.8 cM. Seventy SSR markers were distributed across 17 chromosomes with more than one locus.

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• Current Trends in Wheat Breeding Strategies for Developing Domestic Wheat Cultivars in Korea
  Hajeong Kang, Hyoun-Min Park, San-Gu Lee, Eun-Ha Kim, Muhammad Imran, Hanyoung Choi, Myeong-Ji Kim, Seonwoo Oh
  Korean Journal of Breeding Science.2024; 56(4): 491.     CrossRef
• Genetic mapping of two quantitative resistance loci to Venturia nashicola in an interspecific pear family
  Kyungho Won, Eu Ddeum Choi, Keumsun Kim, Il Sheob Shin, Seongsig Hong, Cécile Segonzac, Kee Hoon Sohn, Cecilia H. Deng, Lester Brewer, David Chagné, Vincent G. M. Bus
  Tree Genetics & Genomes.2024;[Epub]     CrossRef
• Construction of High-Density Genetic Linkage Map Using GBS-SNPs and SSRs in Interspecific Hybrid Derived from the Cross Between ‘Manpungbae’ and ‘Oharabeni’ Pear (Pyrus spp.)
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  Korean Journal of Breeding Science.2022; 54(4): 285.     CrossRef
• Anticipated Polymorphic SSRs and Their Application Based on Next Generation Sequencing of Prunus Persica
  Jung Sun Kim, Yoon Suk Ku, Sin-Gi Park, Se Hee Kim, Hyun Woo Park, So Youn Won
  Korean Journal of Breeding Science.2021; 53(4): 350.     CrossRef