We tried to develop the protocol for embryogenesis and plant regeneration from anther culture of carrot (Daucus carota L.) genotype ‘S&P2342’. Anthers were cultured on MS medium with B5 vitamins containing different combinations of 2,4-D and NAA for 18 weeks in the dark. The best induction of callus and embryo was obtained in the medium containing 0.1 mg/L 2,4-D and 0.1 mg/L NAA, on which 22.0% callus and 2.0% embryo were induced. When primary embryos induced directly from anther culture were transferred to the regeneration medium, secondary embryos were initiated from primary embryos after 4 weeks of culture and 62.5% converted into plantlets after 8 weeks of culture. The plantlets with true leaves were obtained after 12 weeks of culture. When the calli derived from anther culture were transferred to the regeneration medium, 38.8% of the calli produced primary embryos and plantlets after 8 weeks of culture. The plantlets with 2 or more leaves cultured on the regeneration under the different light intensity for the growth of in vitro plantlets. The plantlets cultured at 100 μmol·m^-2·s^-1 showed the highest growth rate. For the acclimatization, the in vitro plantlets with 4 or more leaves cultivated under the different light intensity and temperature, respectively. The survival rate and growth of plantlets was best at 15℃ and 100 μmol·m^-2·s^-1, respectively. The plants were successfully acclimatized and had a normal phenotype. The anther culture system could be used to prepare the doubled haploid lines as an appropriate breeding material for F₁ hybrid breeding program.