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"On sook Hur"

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"On sook Hur"

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Fluidigm 시스템을 이용한 고추 유전자원의 주요병 유전형 평가
Evaluation of Genotypes for Major Diseases of Pepper Genetic Resources Using the Fluidigm System
Bora Geum, Na Young Ro, On sook Hur, Xiaohan Wang, Yu-Mi Choi, Hocheol Ko, Sukyeung Lee
Korean. J. Breed. Sci. 2021;53(4):432-449.
Published online December 1, 2021
DOI: https://doi.org/10.9787/KJBS.2021.53.4.432

In this study, genotype analysis was conducted using the Fluidigm system with 13 SNP markers related to anthracnose, powdery mildew, phytophthora root rot, cucumber mosaic virus (CMV), potyvirus, and tobacco mosaic virus (TMV) resistance. A total of 25,350 data points were obtained, 8.2% of which indicated resistance genotypes. Resources having perfect resistance genotype to each disease-related molecular marker set were 58 accessions for anthracnose, 57 for powdery mildew, 246 for phytophthora root rot, and 765 for CMV. There was no resource having a perfect resistance genotype of all three potyvirus-related molecular SNP markers including pvr1, pvr2 (123457), and pvr2 (689). Thirty-seven accessions for pvr1, 349 accessions for pvr2 (123457), and 23 accessions for pvr2 (689) were identified as resources with resistance genotype. No resource with the TMV-resistance genotype was found in this study. The resources with complex resistance genotypes of anthracnose and powdery mildew were 55 accessions, and they were mainly Capsicum baccatum. Among these, seven accessions had a three-disease combined resistance genotype with phytophthora root rot. Thirty-six accessions, 16 accessions, and nine accessions each had resistance genotypes of the three potyvirus-related markers. These resources also had CMV and phytophthora root rot resistance genotypes, and they were mainly Capsicum chinense. The statistical analysis considering the evaluated resistance genotypes, revealed that the anthracnose resistance genotype showed a tendency to be linked with powdery mildew resistance genotype. The potyvirus-related marker pvr1 was positively correlated with Phytophthora root rot markers.

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주요영양성분과 농업적 특성 분석을 통한 한중일 귀리( L.) 유전자원의 원산지별 다양성 비교
Comparison of the Diversity of East Asian Oat (Avena sativa L.) Genetic Resources by Origins, Considering Major Nutritional Ingredients and Agronomic Traits
Sukyeung Lee, Hyemyeong Yoon, Myung-chul Lee, Sejong Oh, Muhammad Rauf, On sook Hur, Na Young Ro, Jungyoon Yi, Do yoon Hyun, Gyu Taek Cho, Hocheol Ko, Yu-Mi Choi
Korean. J. Breed. Sci. 2019;51(1):9-19.   Published online March 1, 2019
DOI: https://doi.org/10.9787/KJBS.2019.51.1.9

Oats are known for their abundance of quality proteins and lipids compared to those of other grain crops; they especially contain a large amount of good quality fibers, including β-glucan. The Korean domestic consumption of oats is rapidly increasing due to their high nutritional value. However, the research for functional food breeding material has been insufficient because oats have been focused on as feed. Therefore, this study aimed to evaluate the agronomic traits and important nutritional ingredients, including β-glucan, protein, lipid, fiber, and dietary fiber, of 142 oat germplasms from Korea, China, and Japan, which are maintained in the National Agrobiodiversity Center. In addition, the diversity by origin of the germplasms was analyzed based on their agronomic traits. For the agronomic characteristics, Korean oat germplasms were on average headed and matured earlier than the Chinese and Japanese ones. Seven accessions, including IT151107, were matured before mid-June, which makes double cropping possible in the Southern region of Korea. In the diversity analysis by origin based on quantitative agronomic traits, Korean oat germplasms were in the distinguished group compared to Chinese and Japanese germplasms. Oat germplasm had a wider range of main ingredients compared to that of Korean domestic oat cultivars. However, Chinese germplasms had a significantly higher β-glucan content, while Korean germplasms had significantly higher crude protein, crude lipid, and crude fiber contents, than did other germplasms from other origins. Dietary fiber contents showed no significant differences between origins. IT129802 (China), IT166594 (Korea), IT166584 (Korea), IT151108 (Korea), and IT129798 (China) showed the highest β-glucan, protein, lipid, fiber, and dietary fiber contents, respectively. These selected oat germplasms can be superior breeding materials for various functional and agronomic purposes.

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동아시아 재래종 밀( L.) 유전자원의 고분자 글루테닌 조성과 숙기 특성 평가
Evaluation of East Asian Landrace Wheat Revealed by High Molecular Weight Glutenin and Maturity Period
Sukeyung Lee, Yu-Mi Choi, Do yoon Hyun, Myung-chul Lee, Sejong Oh, On sook Hur, Hocheol Ko, Yeonju Jung
Korean. J. Breed. Sci. 2015;47(3):264-275.   Published online September 30, 2015
DOI: https://doi.org/10.9787/KJBS.2015.47.3.264

This study is to raise the utilization of genetic resources of wheat (Triticum aestivum L.) landrace from East Asia (Korea, Japan, and China) by evaluating genetic variation related to end use quality concerned to bread making quality and early maturity, two very important characteristics of Korean wheat cropping system. To clarify the allelic variation of Glu-1 loci which decides HMW glutenin subunit composition, SDS-PAGE and DNA marker analysis were conducted by using 485 East Asian landrace wheat accessions in National Agrobiodiversity Center, RDA and NIAS genebank. And useful accessions getting full mark of glu-1 score and early maturity were selected to enhance the utilization of genetic resources to Korean wheat breeding.

In this study, wheats from China showed the distinct characteristic. Whereas Glu-A1c (null) and Glu-B1b (7+8) allele are the most frequent in Korean and Japanese accessions, Glu-A1a (2*) and Glu-B1c (7*+9) are the most in Chinese accessions. When it comes to unique composition, Glu-B1f (13+16) and Glu-D1e (2+10) subunits are only in Chinese resources. Glu-B1d (6+8), Glu-B1e (20), Glu-D1b (7+8), and Glu-D1c (4+1) subunits are only in Korean resources. The accessions from China also has high PIC value (0.53) compared to ones from Korea (0.35) and Japan (0.35). Grouping by UPGMA analysis of combination of Glu-1 allele, most accessions from Korea and Japan are in the same group, but most Chinese ones were distinguished as the distinctive group. The evaluation of bread baking quality by Glu-1 scoring system, 26 accessions got full marks. Among them, 16 accessions from China were also matured before early June, suitable to Korean cropping system. Especially, 3 accessions (K151847, K151865, K151962) had very early maturity, matured in late May. These genetic resources, having good gluten composition and early maturity, are expected to widely be used for Korean wheat breeding.

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