Molecular markers of doubled-haploid (DH) and long spike Korean wheat cultivar populations were evaluated to improve the yield potential of Korean wheat breeding programs by improving the tiller numbers. In Korean wheat cultivars, the tiller number was affected more by vernalization (Vrn) and photoperiod (Ppd) than by tiller inhibition gene (tin) and fertile tiller inhibition gene (ftin). Also, it was negatively correlated with the yield. In DH lines, the tiller number was significantly affected by neither the tiller inhibition genes nor Vrn and Ppd. Additionally, it was not significantly correlated with the yield. However, the adaptability of both SSR markers, Xgpw2206 and Xgwm247, was higher in the DH lines than in the other populations. In the long spike population, the tiller number differed according to the Ppd-D1 allele, affecting the yield. In this study, genes and molecular markers related to the tiller number, as described in previous studies, might be difficult to use to evaluate the tiller number and yield of the Korean wheat population. Hence, continuous study is necessary to develop molecular markers suitable for improving the tiller number and yield of Korean wheat cultivars.