In this study, conducted in living modified organism (LMO) isolation fields, we sought to develop environmental risk assessment procedures for identifying the potential effects on non-target above-ground insects and spiders within agroecosystems cultivated with vitamin A-enhanced transgenic soybean with tolerance to the herbicide glufosinate. To this end, we investigated insect/arachnid species diversities and population densities on vitamin A-enhanced transgenic soybean and non-GM soybean (Gwangan) grown in LMO quarantine areas of Kyungpook National University (Gunwi) and the National Institute of Agricultural Sciences (Jeonju). In total, 93,419 individual insects and arachnids, representing 65 families in 12 orders, were captured during the study. In Gunwi, totals of 17,110 and 17,627 individual insects and arachnids were collected from vitamin A-enhanced transgenic soybean and Gwangan, respectively, whereas in Jeonju, totals of 28,621 and 30,061 individuals were collected from vitamin A-enhanced transgenic soybean and Gwangan, respectively. Although we detected no significant differences among the population densities of insect pests, natural enemies, and other insects on vitamin A-enhanced transgenic soybean and Gwangan grown within the same field, the population densities of these insects were found to be higher in Jeonju than those in Gunwi. Throughout the study, analysis of variance indicated no significant differences (p<0.05) in insect/arachnid populations, and multivariate analysis indicated that the abundance and diversity of plant-dwelling insects were similar within the same fields.