Korean Journal of Breeding Science

"candidate genes"

Articles

밀 종실 단백질 함량 관련 양적형질유전자좌(QTL) 분석 및 마커 탐색 Quantitative Trait Loci for Grain Protein Content in Wheat (Triticum aestivum L.): Min-Ju Kim, Myoung-Goo Choi, Go-Eun Lee, Chon-Sik Kang, Chang-Hyun Choi, Jun-Seok Choi, Myoung-Hui Lee, Jae-Han Son; Korean. J. Breed. Sci. 2026;58(2):109-122.
Published online June 1, 2026; DOI: https://doi.org/10.9787/KJBS.2026.58.2.109

Abstract
PDF

Grain protein content (GPC) is a quantitative trait that strongly influences wheat processing quality and end-product performance, but is highly affected by environmental conditions. This study aimed to identify QTLs associated with GPC using Korean wheat resources and assess the usefulness of linked markers for selection. A recombinant inbred line (RIL) population (F₇ and F₈; n=359) derived from ‘Joongmo2008’ × ‘Joah’ was genotyped with a 35 K SNP array, and QTL mapping was conducted using 4,891 filtered SNPs. Six QTLs were detected, and a major QTL on chromosome 3 B (Qgpc-3B) was consistently identified in both generations. Within the Qgpc-3B interval, lines carrying the ‘Joongmo2008’ alleles at AX-95163812 and AX-94495360 showed higher GPC, and the same trend was confirmed by a two-marker haplotype analysis. The direction of marker effects was also reproduced in a Korean wheat germplasm panel, suggesting that these markers have strong potential as practical selection indices for improving GPC in wheat breeding programs.

356 View
19 Download

온대자포니카 벼 품종의 등숙기 종자 내 카드뮴 함량에 관한 전장유전체연관분석 Genome-wide Association Study on Cadmium Contents of Temperate Japonica Varieties: Sang-Beom Lee, Kyu-Won Kim, Min-Young Yoon, Gyeong-Jin Kim, Ji-Hyock Yoo, Won-Il Kim, Byeong-Churl Moon, Sang-Won Park, Yong-Jin Park; Korean. J. Breed. Sci. 2016;48(4):470-485. Published online December 31, 2016; DOI: https://doi.org/10.9787/KJBS.2016.48.4.470

Abstract
PDF

Objective of this study was to investigate the difference of cadmium (Cd) levels in rice grains from non-polluted fields and to define the gene associated with Cd uptake for producing safety food. Cd was analyzed by Inductively Coupled Plasma Mass Spectrometer (ICP-MS). The average concentration of Cd in rice grains was 0.943 μg/kg and Cd levels ranged from 0.050 to 5.699 μg/kg. Genome-Wide Association study (GWAS) based on phenotype data for Cd levels was performed. However, results of GWAS were affected by subpopulation structure and caused false positive. Therefore, GWAS for rice ecotypes (temperate Japonica, tropical Japonica, Indica, Aus, Aromatic, and Admixture) was performed to minimize false positive. GWAS results showed that Os01g0611300, Os01g0611900, Os01g0611950, Os01g0612000, Os01g0612200, Os11g0444400, Os11g0444700, Os11g0444800, and Os11g0444900 genes have significant correlation with Cd levels in rice grains. The sequences of these genes were compared to sequence positions of each other gene (haplotype analysis). According to the results of haplotype analysis, Cd levels of non-synonymous group were higher than other groups and sequence of non-synonymous group was similar to that of Indica. These results were corresponding to the previous research result that Cd levels of Indica were higher than Japonica. Therefore, candidate genes detected through GWAS need to be examined by knock-out or cross breeding.

Citations

Citations to this article as recorded by

Unraveling New Genetic Elements Associated with the Morphological Changes and Relative Silicon Content in Rice Using Genome-Wide Association Studies (GWAS)
Giang Son Tran, Quynh Hoa Nguyen, Linh Thi Thuy Nguyen, Huong Mai, Quyen Phan, Minh Ngoc Nguyen, Si Son Tong, Phat Tien Do, Huong Thi Mai To
Journal of Plant Biology.2024; 67(6): 467. CrossRef
Genome-Wide Association Study of Rice Core Set Related to Grain Weight
Buung Choi, Ji-Min Yoo, Sang-Beom Lee, Gyeong-Jin Kim, Kyu-Won Kim, Ji-Hyock Yoo, Kyeong-Seok Oh, Byeong-Churl Moon, Wook-Han Kim, Yoo-Hyun Cho, Sang-Won Park, Yong-Jin Park
Plant Breeding and Biotechnology.2018; 6(3): 293. CrossRef