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"pre-harvest sprouting"

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"pre-harvest sprouting"

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In wheat, pre-harvest sprouting (PHS) is the premature germination of seeds in the spike that occurs before harvest under prolonged wet and humid conditions, causing substantial losses in yield and quality. Moreover, climate change may increase the risk of PHS in wheat production in Korea. In this study, using 42 Korean wheat cultivars, we sought to determine whether the activity of α-amylase could serve as a biochemical indicator of PHS. Spikes were harvested 38 days after anthesis and treated to induce PHS, and after 4 days, we determined the rates of PHS and α-amylase activity among the assessed cultivars. The rates of PHS and activity of α-amylase ranged from 0% to 84.17% and 0.52 to 56.40 SD units/10 g, respectively, and we detected a strong positive correlation between the rate of PHS and α-amylase activity (Pearson’s r=0.90, p<0.001). Simple linear regression further revealed that the activity of α-amylase explained 82% of the variation in the rate of PHS (R2=0.82). These finding indicate that the activity of α-amylase can serve as a quantitative index of PHS severity and complement visual assessments. Consequently, the activity of α-amylase could represent a useful selection criterion for screening PHS resistance among Korean wheat cultivars and in breeding programs.
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The rice cultivar ‘Chamdongjin’ exhibits a distinctively large grain size and excellent eating quality but is weak with regard to pre-harvest sprouting (PHS), requiring genetic improvement. In contrast, the premium-quality rice cultivar ‘Younghojinmi’ exhibits strong PHS tolerance. This study conducted quantitative trait loci (QTL) analysis on PHS and low-temperature germinability (LTG) using recombinant inbred lines derived from a cross between ‘Chamdongjin’ and ‘Younghojinmi’ to elucidate the genetic factors. Thus, an elite line with improved PHS tolerance was selected. QTL analysis revealed that qPHS3 and qLTG3 were associated with PHS and LTG, respectively, and the candidate gene was identified as qLTG3-1. The loss-of-function allele qltg3-1 in ‘Younghojinmi’ enhanced PHS tolerance compared to the functional allele qLTG3-1 in ‘Chamdongjin,’ but it also showed a trade-off relationship by decreasing LTG. CY_RILs carrying qltg3-1 exhibited lower PHS and LTG rates of 17.9% and 41.6%, respectively, compared to 44.1% and 55.7%, respectively, for CY_RILs with qLTG3-1. The elite line, CY_RIL79, carrying qltg3-1, was selected from CY_RIL. This line was recognized for its PHS tolerance while maintaining the key agricultural traits of ‘Chamdongjin,’ thus designated ‘Jeonju697.’ ‘Jeonju697’ is expected to be utilized as a practical alternative for addressing the issue of enhancing PHS tolerance in ‘Chamdongjin’; however, considering its weak LTG, it is desirable to improve this trait through further breeding efforts.

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  • Quantitative Trait Locus Analysis for Quality-Related Traits Using the Recombinant Inbred Lines Derived from a Cross between “Boramchan” and “Pecos” Japonica Rice
    Hyun-Su Park, Chang-Min Lee, Jeonghwan Seo, Songhee Park, Hyeonso Ji, Keon-Mi Lee, Jae-Ryoung Park, O-Young Jeong
    Korean Journal of Breeding Science.2025; 57(4): 373.     CrossRef
  • ‘Amissal’: A Region-specific, Mid-late Maturing Long-grain Japonica Rice Cultivar
    Hyun-Su Park, Chang-Min Lee, Ki-Young Kim, O-Young Jeong, Ji-Ung Jeung, Su-Keyong Ha, Sang-Chul Park, Sang-Hyeok Lee, Jung-Pil Suh, Mina Jin, Hyun-Sook Lee, Jeonghwan Seo, Songhee Park, Jae-Ryoung Park, Kyeongmin Kang
    Korean Journal of Breeding Science.2025; 57(4): 547.     CrossRef
  • Quantitative Trait Loci Analysis of Quality-Related Traits Using Recombinant Inbred Lines Derived from a Cross between ‘Chamdongjin’ and ‘Younghojinmi’
    Hyun-Su Park, Jeonghwan Seo, Chang-Min Lee, Songhee Park, Keon-Mi Lee, Jae-Ryoung Park, O-Young Jeong
    Korean Journal of Breeding Science.2024; 56(4): 395.     CrossRef
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대량전사체분석을 통한 국내 수발아 저항성 밀의 유전자 발현 분석
Expression Analysis of Pre-Harvest Sprouting Tolerant Korean Wheat via Transcriptomic Analysis
Sang Yong Park, Chang Hyun Choi, Kyung Hoon Kim, Woo Joo Jung, Jae Yoon Kim
Korean. J. Breed. Sci. 2022;54(2):104-118.
Published online June 1, 2022
DOI: https://doi.org/10.9787/KJBS.2022.54.2.104

Globally, wheat (Triticum aestivum) is a major food crop for humans with no regional restrictions. However, it is still difficult for Korea to achieve self-sufficiency owing to production limitations. Moreover, food security is unstable owing to the unpredictable climate and unstable international economy. Pre-harvest sprouting (PHS) is among the factors that occurs frequently due to irregular climates, and damages the value of wheat. In this study, RNA-seq was conducted on PHS-treated samples (for Korean representative cultivar ‘keumgang’) and PHS-resistant mutation line ‘Jeonju 377ho’. Gene functional annotation and DEGs analysis were performed using 234,131,980 mapped reads. Associated transcripts were analyzed using Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and were mainly used to search for genes associated with ATP synthesis and starch and sucrose metabolism related to seed germination and seed dormancy. Candidate DEGs were compressed through cluster set analysis, and gene expression was conducted to search for genes related to seed germination and dormancy to explain them in greater detail based on biological and chemical mechanisms.

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  • Transcriptome Analysis for Flooding Stress-related Gene Identification inGlycine soja
    Tae Kyeom Kim, Sang Yong Park, Jae Yoon Kim
    Korean Journal of Breeding Science.2022; 54(4): 315.     CrossRef
  • Transcriptome and Proteome Co-Profiling Offers an Understanding of Pre-Harvest Sprouting (PHS) Molecular Mechanisms in Wheat (Triticum aestivum)
    Sang Yong Park, Woo Joo Jung, Geul Bang, Heeyoun Hwang, Jae Yoon Kim
    Plants.2022; 11(21): 2807.     CrossRef
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벼흰잎마름병 저항성 고품질 중생 벼 ‘해품’
Bacterial Blight-Resistant Medium Maturing Rice Cultivar ‘Haepum’ with High Grain Quality
Jeong-Kwon Nam, Hyun-Su Park, Man-Kee Baek, Young-Chan Cho, Woo-Jae Kim, Jeong-Ju Kim, Bo-Kyeong Kim, Ki-Young Kim, Woon-Chul Shin, Jong-Cheol Ko, Gun-Mi Lee, Seul-Gi Park, Chang-Min Lee, Choon-Song Kim, Jung-Pil Suh, Jeom-Ho Lee
Korean. J. Breed. Sci. 2019;51(3):222-233.   Published online September 1, 2019
DOI: https://doi.org/10.9787/KJBS.2019.51.3.222

‘Haepum’ is a bacterial blight-resistant, medium maturing rice cultivar with high grain quality. It was derived from a cross between ‘Iksan493’ (cultivar name ‘Jinbaek’) and the F1 cross between ‘Iksan495’ (‘Dacheong’) and ‘Iksan496’ (‘Jungmo1005’). Of these three cultivars, ‘Jinbaek’ is a bacterial blight-resistant mid-late maturing rice cultivar with high grain quality, ‘Dacheong’ is a mid-late maturing rice cultivar with multiple resistance to disease and insects, and ‘Jungmo1005’ is a mid-late maturing rice cultivar with lodging tolerance. To develop fixed lines, the anther culture method was applied to F1 plants. The cultivar ‘Haepum’ was selected using the pedigree method, yield trials, and local adaptability tests. The heading date of ‘Haepum’ was August 11th, three days earlier than that of ‘Nampyeong’ cultivar. Haepum’ is a cultivar tolerant to lodging and it has short culms. Due to its low rate of viviparous germination, ‘Haepum’ could be useful for preventing pre-harvest sprouting in cultivation of medium maturing rice in the southern plain area of Korea. ‘Haepum’ carries two bacterial blight resistance genes (Xa3 and xa5), and in our study, it exhibited high-level and broad-spectrum resistance against bacterial blight, including K3a, the most virulent race in Korea. ‘Haepum’ is also resistant to the rice stripe virus and moderately resistant to rice blast. The yield of ‘Haepum’ was similar to that of ‘Nampyeong’. ‘Haepum’ showed excellent grain appearance and good taste of cooked rice, and therefore it could contribute to the development of bacterial blight-resistant rice cultivars of improved quality. ‘Haepum’ would be suitable for cultivation in the southern plain area of Korea as well as in bacterial blight-prone areas. (Registration No. 6068)

Citations

Citations to this article as recorded by  
  • QTL Analysis for Yield and Grain-Related Traits Using the Recombinant Inbred Lines Derived from a Cross between ‘Boramchan’ and ‘Pecos’
    Hyun-Su Park, Chang-Min Lee, Jeonghwan Seo, Songhee Park, Keon-Mi Lee, Jae-Ryoung Park, O-Young Jeong
    Korean Journal of Breeding Science.2025; 57(2): 131.     CrossRef
  • High throughput phenotyping using automated imaging system reveals the relationship between seed yield and agronomic traits in Korean rice cultivars
    Joon Ki Hong, Jeongho Baek, Jae Young Kim, Song Lim Kim, Jae Il Lyu, Sang-Ho Kang, Jiseon Song, Nyunhee Kim, Eunsook An, Hyun-Sook Lee, Kyung-Hwan Kim, Yong Suk Chung, Sheikh Mansoor
    Journal of Plant Physiology.2025; 311: 154544.     CrossRef
  • Predicting rice yield at pixel scale through synthetic use of crop and deep learning models with satellite data in South and North Korea
    Seungtaek Jeong, Jonghan Ko, Jong-Min Yeom
    Science of The Total Environment.2022; 802: 149726.     CrossRef
  • Characterization of Quality-Related Traits and Pasting Properties of Early Maturing Rice Varieties by Cultivation Times in the Honam Plain, Korea
    Hyun-Su Park, Man-Kee Baek, Chang-Min Lee, Suk-Man Kim, Jung-Pil Suh, O-Young Jeong, Young-Chan Cho
    Korean Journal of Breeding Science.2021; 53(1): 1.     CrossRef
  • Future Changes of Agro-Climate and Heat Extremes over S. Korea at 2 and 3 °C Global Warming Levels with CORDEX-EA Phase 2 Projection
    Sera Jo, Kyo-Moon Shim, Jina Hur, Yong-Seok Kim, Joong-Bae Ahn
    Atmosphere.2020; 11(12): 1336.     CrossRef
  • High Grain Quality Mid-late Maturing Rice Cultivar ‘Yechan’ with Lodging Tolerance and Multiple Disease Resistance
    Man-Kee Baek, Hyun-Su Park, Jeong-Kwon Nam, Young-Chan Cho, Ki-Young Kim, Jeong-Ju Kim, Woo-Jae Kim, Woon-Chul Shin, Ji-Ung Jeung, Choon-Song Kim, Jong-Min Jeong, Keon-Mi Lee, Seul-Gi Park, Chang-Min Lee, Jung-Pil Suh, Jeom-Ho Lee
    Korean Journal of Breeding Science.2019; 51(4): 504.     CrossRef
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벼흰잎마름병 저항성 고품질 중만생 벼 ‘만백’
Bacterial Blight Resistant Mid-late Maturing Rice ‘Manbaek’ with High Grain Quality
Hyun-Su Park, Man-Kee Baek, Bo-Kyeong Kim, Ki-Young Kim, Woon-Chul Shin, Jae-Kwon Ko, Jeong-Kwon Nam, Woo-Jae Kim, Young-Chan Cho, Jong-Cheol Ko, Jeong-Ju Kim, Hyun-Soon Kim
Korean. J. Breed. Sci. 2017;49(3):235-244.   Published online September 1, 2017
DOI: https://doi.org/10.9787/KJBS.2017.49.3.235

‘Manbaek’ is a bacterial blight resistant mid-late maturing rice cultivar with high grain quality. ‘Manbeak’ was derived from anther culture using the backcross combination, Hopum*2/SR30075. ‘Hopum’ is a mid-late maturing rice cultivar with high grain quality and ‘SR30075’ is a pyramid line carrying three bacterial blight resistance genes. ‘Manbaek’ was selected through the pedigree method, yield trials, and local adaptability tests. ‘Manbeak’ carrying two bacterial blight resistance gene Xa3 and xa5 showed high-level and broad-spectrum resistance against bacterial blight. ‘Manbaek’ was resistant to K3a, mostly virulent race in Korea, and exhibited resistance reaction against 16 Korean bacterial blight isolates. ‘Manbaek’ was a mid-late maturing rice. The heading date of ‘Manbaek’ was August 19th, which was 5 days later than that of ‘Nampyeong’. Manbaek’ was a lodging-tolerant rice with short culm and dark green leaf. Due to the low viviparous germination, ‘Manbaek’ could be a useful material to prevent pre-harvest sprouting. ‘Mabeak’ was resistant to bacterial blight and rice stripe virus, but susceptible to other virus diseases and insect pests. The yield of ‘Manbaek’ was similar to ‘Nampyeong’. ‘Manbaek’ showed excellent grain appearance and good tastes of cooked rice, so that it could contribute to improving the quality of bacterial blight resistant cultivars. ‘Manbaek’, bacterial blight resistant cultivar with high grain quality, is suitable for the cultivation at bacterial blight prone area and has been utilized in the breeding programs for enhancing the resistance against bacterial blight (Registration No. 6069).

Citations

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  • Phenotypic and transcriptomic characterization of OsSWEET14-edited rice (cv. Samkwang) with enhanced bacterial blight resistance
    Yeeun Jun, Jiheon Han, Yurim Kim, Sieun Choi, Song Lim Kim, Jae-Hyeon Oh, Eun-Jung Suh, Sang-Ho Kang, Hye-Ran Park, Hyun-Cheol Jeong, Sang Ryeol Park, Youngjun Mo
    BMC Plant Biology.2025;[Epub]     CrossRef
  • QTL Analysis for Pre-Harvest Sprouting and Low-Temperature Germinability Using Recombinant Inbred Lines Derived from a Cross between ‘Chamdongjin’ and ‘Younghojinmi’
    Hyun-Su Park, Jeonghwan Seo, Heyonso Ji, Gileung Lee, Chang-Min Lee, Jae-Ryoung Park, Songhee Park, Keon-Mi Lee, Mina Jin, O-Young Jeong
    Korean Journal of Breeding Science.2024; 56(2): 79.     CrossRef
  • Multiple Disease Resistant Early Maturing Rice Cultivar ‘IS592BB’ with the Genetic Background of ‘Unkwang’
    Hyun-Su Park, Man-Kee Baek, Woo-Jae Kim, Chang-Min Lee, Hyeonso Ji, Jung-Pil Suh, O-Young Jeong, Young-Chan Cho, Jeom-Ho Lee
    Korean Journal of Breeding Science.2020; 52(4): 473.     CrossRef
  • Bacterial Blight Resistance Genes Pyramided in Mid-Late Maturing Rice Cultivar ‘Sinjinbaek’ with High Grain Quality
    Hyun-Su Park, Ki-Young Kim, Man-Kee Baek, Young-Chan Cho, Bo-Kyeong Kim, Jeong-Kwon Nam, Woon-Chul Shin, Woo-Jae Kim, Jong-Cheol Ko, Jeong-Ju Kim, Jong-Min Jeong, Ji-Ung Jeung, Keon-Mi Lee, Seul-Gi Park, Chang-Min Lee, Choon-Song Kim, Jung-Pil Suh, Jeom-H
    Korean Journal of Breeding Science.2019; 51(3): 263.     CrossRef
  • Bacterial Blight-Resistant Medium Maturing Rice Cultivar ‘Haepum’ with High Grain Quality
    Jeong-Kwon Nam, Hyun-Su Park, Man-Kee Baek, Young-Chan Cho, Woo-Jae Kim, Jeong-Ju Kim, Bo-Kyeong Kim, Ki-Young Kim, Woon-Chul Shin, Jong-Cheol Ko, Gun-Mi Lee, Seul-Gi Park, Chang-Min Lee, Choon-Song Kim, Jung-Pil Suh, Jeom-Ho Lee
    Korean Journal of Breeding Science.2019; 51(3): 222.     CrossRef
  • Multiple Disease Resistant Early Maturing Rice Cultivar 'Joil' with High Grain Quality Adaptable to Early Transplanting Cultivation in the Southern Plain Area
    Jeong-Kwon Nam, Hyun-Su Park, Ki-Young Kim, Bo-Kyeong Kim, Young-Chan Cho, Jae-Kwon Ko, Man-Kee Baek, Jeong-Ju Kim, Woo-Jae Kim, Woon-Chul Shin, Jong-Cheol Ko, Jeom-Ho Lee, Jong-Min Jeong, Ji-Ung Jeung, Kyung-Ho Kang
    Korean Journal of Breeding Science.2018; 50(3): 280.     CrossRef
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New Cultivar Developed

A Wheat Variety, ‘Sooan’ with Good Noodle Quality, Red Grain Wheat, Higher Winter Hardiness and Pre-harvest Sprouting Resistance
Chon-Sik Kang1, Young-Keun Cheong1, Kyeong-Hoon Kim1, Hag-Sin Kim1, Young-Jin Kim1, Kyong-Ho Kim1, Jong-Chul Park1, Hyung-Ho Park1, Hong-Sik Kim2, Sung-Ju Kang3, Hong-Jip Choi4, Jung-Gon Kim5, Kee-Jong Kim1, Choon-Ki Lee1, Kwang-Geun Park1, Ki-Hun Park1, and Chul-Soo Park6*
Korean. J. Breed. Sci. ;46(3):260-267.   Published online September 30, 2014
DOI: https://doi.org/10.9787/KJBS.2014.46.3.260
‘Sooan’, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross ‘Keumkang/Eunpa//Keumkang’ during 1997. ‘Sooan’ was released from the line named as ‘Iksan319’ in 2009 after yield trials test in conducted for three years from 2007. It was tested in the regional yield trial between 2007 and 2009. ‘Sooan’ is an awned, semi-dwarf and hard winter wheat, similar to ‘Keumkang’ (check cultivar). The heading and maturing date of ‘Sooan’ were similar to ‘Keumkang’. ‘Sooan’ had similar test weight (817 g/L) and lower 1,000-grain weigh (43.7 g) than ‘Keumkang’ (817 g/L and 46.2 g, respectively). ‘Sooan’ showed resistance to winter hardiness and pre-harvest sprouting, which lower withering rate on the low ridge (0.2%) and rate of pre-harvest sprouting (0.2%) than ‘Keumkang’ (9.2 and 25.8%, respectively). ‘Sooan’ showed lower protein content (12.7%), SDS-sedimentation volume (42.0 ml) and gluten content (10.5%) than ‘Keumkang’ (13.1%, 57.8ml and 10.8%, respectively). It showed higher lightness (90.08) and lower redness (-1.11) and yellowness (8.80) in flour color than ‘Keumkang’ (89.81, -1.15 and 9.46, respectively). ‘Sooan’ showed higher lightness (81.34) of noodle dough sheet than ‘Keumkang’ (80.28). ‘Sooan’ exhibited lower hardness (3.84N) and similar springiness and cohesiveness of cooked noodles (0.91 and 0.65) compared to ‘Keumkang’ (4.54N, 0.93, and 0.63, respectively). Average yield of ‘Sooan’ in the regional adaptation yield trial test was 5.94 kg/10a in upland and 5.63 kg/10a in paddy field, which was 3% and 8% higher than those of ‘Keumkang’ (579 kg/10a and 520 kg/10a, respectively).

Citations

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  • Gene expression analysis of wheat (Triticum aestivum L.) during the vegetative stage under high ambient temperature condition
    Hyeju Seong, Ji Hye Heo, Woosuk Jung
    Journal of Crop Science and Biotechnology.2024; 27(2): 261.     CrossRef
  • Expression Analysis of Pre-Harvest Sprouting Tolerant Korean Wheat via Transcriptomic Analysis
    Sang Yong Park, Chang Hyun Choi, Kyung Hoon Kim, Woo Joo Jung, Jae Yoon Kim
    Korean Journal of Breeding Science.2022; 54(2): 104.     CrossRef
  • Physicochemical Properties of Various Wheat Flours by Sowing Time
    Jang Keun Son, Ho Jun Sung, Hye Won Lee, Yeon Jae Jo, Ji Eun Kwak, Tae Su Kang, Junsoo Lee, Heon Sang Jeong
    Journal of the Korean Society of Food Science and Nutrition.2022; 51(8): 805.     CrossRef
  • Transcriptome and Proteome Co-Profiling Offers an Understanding of Pre-Harvest Sprouting (PHS) Molecular Mechanisms in Wheat (Triticum aestivum)
    Sang Yong Park, Woo Joo Jung, Geul Bang, Heeyoun Hwang, Jae Yoon Kim
    Plants.2022; 11(21): 2807.     CrossRef
  • Quality Characteristics of Bread Made from Domestic Early Maturity and High Yield Wheat Cultivars
    Tae Yong You, Yeon Jae Jo, Tae Hwan Shin, Ji Eun Kwak, Yun Jeong Lee, Tae Su Kang, Junsoo Lee, Heon Sang Jeong
    Journal of the Korean Society of Food Science and Nutrition.2021; 50(12): 1358.     CrossRef
  • Past and Current Status, and Prospect of Winter Cereal Crops Research for Food and Forage in Korea
    Kyeong-Min Kim, Chon-Sik Kang, Yang-Kil Kim, Kyeong-Hoon Kim, Jong-Ho Park, Young-Mi Yoon, Hyoung-Ho Park, Han-Yong Jeong, Chang-Hyun Choi, Jinhee Park, Young-Jin Kim, Young-Keun Cheong, Ouk-Kyu Han, Tae-Il Park
    Korean Journal of Breeding Science.2020; 52(S): 73.     CrossRef
  • Effects of Various Seeding Type and Ratios of Wheat Varieties on Agricultural Characteristics and Quality of Plants
    Na-Ri Lee, Kyeong-Hoon Kim, Kyeong-Min Kim, Sang-Hack Lee, Young-Up Kwon, Jong-Nae Hyun
    The Korean Journal of Crop Science.2016; 61(2): 98.     CrossRef
  • Quality Characteristics of Wheat Flours from New Released Iksan370 with Long Spike and Domestic Wheat Cultivars
    Yong-Seok Choi, Jae-Kang Lee, Yong-Hyun Choi, Young-Hwan Kim, Cheon-Sik Gang, Malsik Shin
    Korean journal of food and cookery science.2015; 31(5): 551.     CrossRef
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