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"tissue culture"

New Cultivar Developeds

거대배 복합 내병충성 벼 품종 ‘드리미4호’ 육성
Breeding a Giant Embryo and Multi-disease-resistant Rice Cultivar, ‘Drimi4ho’
Eun-Gyeong Kim, Jae-Ryoung Park, Yoon-Hee Jang, Jae-Keun Sohn, Gang-Seob Lee, Kyung-Min Kim
Korean. J. Breed. Sci. 2023;55(1):36-47.
Published online March 1, 2023
DOI: https://doi.org/10.9787/KJBS.2023.55.1.36

Rice is an important food crop that feeds most of the world's population. However, due to the increased frequency of pest outbreaks, it is necessary to cultivate complex pest-resistant rice cultivars. A mutant population was derived by culturing tissue of ‘Hwayeong’ at the Plant Molecular Breeding laboratory of Kyungpook National University in the Republic of Korea. The cultivar ‘Drimi4ho’, a line that was resistant to complex disease, was finally selected by backcrossing with ‘Junam’. The heading date of ‘Drimi4ho’ is August 11 (107 days after sowing). Drimi4ho’ has culm length of 69 cm, panicle length of 21 cm, 16 panicles per hill, 115 spikelets per panicle, a ratio of ripened grain of 92.7%, and a 1,000-grain weight of 24.3 g. No lodging occurred when ‘Drimi4ho’ was grown in the field by standard fertilization. It is also resistant to leaf/neck blast disease, bacterial blight (K1, K2, K3), rice dwarf virus, rice black-streaked dwarf virus, rice stripe virus, BPH, and SBPH. ‘Drimi4ho’ is a round and short-type grain, and is very clear and transparent without chalkiness. In addition, it has excellent physicochemical properties that make up food, so its taste is good. When ‘Drimi4ho’ was planted under ordinary conditions in the southern mountainous area of Korea, the yield was 5.53 MT/ha (Registration No. 6124).

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단백질 함량 증가 고품질 특수미 ‘드리미5호’ 육성
Breeding of a Rice Cultivar “Drimi5ho” with High Protein Content and High-Quality Specialty
Jae-Ryoung Park, Eun-Gyeong Kim, Yoon-Hee Jang, Gang-Seob Lee, Kyung-Min Kim
Korean. J. Breed. Sci. 2022;54(4):421-432.
Published online December 1, 2022
DOI: https://doi.org/10.9787/KJBS.2022.54.4.421

Rice is a staple food in most countries, and thus, diversifying its value is necessary. Recently, the focus on good health has increased, and rice cultivars with improved properties should be bred based on consumer demands. At the Plant Molecular Breeding Laboratory of Kyungpook National University, “Hwayeong” was tissue-cultured to derive mutant populations. In the mutant lines, “P35” with high protein content and excellent agricultural traits, was backcrossed with “Ilmi” three times, and the final selected line was called “Drimi5ho.” The heading date of “Drimi5ho” was August 11 (107 d after sowing), which was 5 d later than that of “Ilmi” (August 5, 101 d after sowing). The culm length of “Drimi5ho” was 71 cm, the panicle length was 18 cm, the number of panicles per hill was 13, the number of spikelets per panicle was 113, the ratio of ripened grain was 90.5%, and the 1,000-grain weight was 22.6 g. When "Drimi5ho" was planted ordinary plantation, the yield in the southern mountainous area was 526 kg/10a. Lodging was not observed when “Drimi5ho” was grown in the field with standard fertilization (N-P2O5-K2O = 9-4.5-5.7 kg/10a). This rice cultivar was resistant to leaf/neck blast, bacterial blight (K1, K2, and K3), rice stripe virus, rice black-streaked dwarf virus, and rice dwarf virus. The milled grain of “Drimi5ho” was clear and transparent without any chalkiness; moreover, the grain shape was round and short. Although it is a specialty rice with increased protein content, the taste of the rice and the grain quality were excellent (Registration No. 6829).

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한국 대표 밀 품종의 봄파종 시기에 따른 미성숙배 채취 시기별 조직배양 효율
The Effects of Harvesting Time on the Efficiency of Tissue Culture Used Immature Embryos from Korean Wheat Cultivars
Changhyun Choi, Jae-Han Son, Jin Hee Park, Kyeong-Min Kim, Kyeong-Hoon Kim, Han-Yong Jung, Ji-Young Shon, Tae-Il Park, Chon-Sik Kang
Korean. J. Breed. Sci. 2020;52(4):382-388.   Published online December 1, 2020
DOI: https://doi.org/10.9787/KJBS.2020.52.4.382

The regeneration rate of plantlets cultivated via tissue culture is an important factor for wheat transformation. Similar to other monocotyledons, the most efficient tissue culture materials for wheat are immature embryos. However, stable year-round production of immature embryos is not possible in the field where various stress factors co-exist. In this study, we investigated the generation and subsequent plantlet incident rates of callus induced from immature embryos obtained from different sowing times in 2020 and compared these among wheat cultivars. We found that the rates of regeneration and plantlet incidence obtained using immature embryos of the Ariheuk cultivar were higher than those of other domestic cultivars, and that the tissue culture efficiency was similar to that of Bobwhite, which has been established as a cultivar with excellent transformation efficiency. Furthermore, the Baekkang cultivar showed high tissue culture efficiency only when sown from early to mid-March, whereas Keumkang showed higher tissue cultivation efficiency only by sowing in mid- and late February. Among the five cultivars assessed in this study, Jopum showed the lowest tissue culture efficiency. It is anticipated that the findings of this study will contribute to enhancing the transformation efficiency of domestic wheat varieties.

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