Flooding stress causes a significant reduction in soybean yield. The development of flood-tolerant cultivars is an effective way to minimize yield loss due to flooding. Information on candidate genes for flooding tolerance is useful for developing tolerant lines. The
objective
of this study was to identify potential candidate genes for flooding tolerance in soybean by integrating the results of a quantitative trait locus analysis and RNA sequencing. A total of 19 genes showed good amplification in capillary electrophoresis and were further analyzed through a reverse transcription quantitative polymerase chain reaction (qRT-PCR); two of these genes showed differential expression among tolerant and susceptible lines. The expression of Glyma.12g030900 and Glyma.10g050300 in leaf and root tissues, respectively, was higher in several tolerant lines than in the susceptible lines under flooding stress. The chlorophyll index of the tolerant lines was also consistently higher than that of the susceptible lines over two years, supporting the qRT-PCR results. This study provides useful information on flooding tolerance in soybeans.

Suppression subtractive hybridization was carried out to identify resistant genes against to pear scab caused by Venturia nashicola using leaves harvested at 24 and 48 hours after inoculation into ‘93-3-98’ (highly resistant) and ‘Sweat Skin’ (highly susceptible). As a result of the ESTs analysis, nine and 14 unique genes were expressed on 24H (tester, mRNA at 24hr after inoculation of ‘93-3-98’; driver, one of ‘Sweet Skin’) and 48H (tester, mRNA at 48hr after inoculation of ‘93-3-98’; driver, one of ‘Sweet Skin’), respectively and genes related to defense or stress response were accounted for 40% (24H) and 42% (48H). Differential expressed genes classifying into PR protein family were pathogenesis-related protein 1a, major allergen Pyr c1 and allergen mal d 1 at 24H and major allergen Mal d 1.03B at 48H, respectively. Major allergen Pyr c1, F-Box/kelch-repeat protein, Flavoprotein wrbA, and hypothetical protein POPTRDRAFT_783792 are expected to closely connecting to scab resistance of pear following strongly expressed in highly resistant cv. ‘Bartlett’ and ‘93-3-98’ compared with moderately susceptible cv. ‘Gamcheonbae’, susceptible cv. ‘Wonhwang’, and highly susceptible cv. ‘Niitaka’ and ‘Sweat skin’.