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Korean. J. Breed. Sci. : Korean Journal of Breeding Science

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"Hyun-Soon Kim"

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"Hyun-Soon Kim"

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복합내병충성 고품질 중만생 벼 ‘중모1045호’
Brown Planthopper and Bacterial Blight Resistant Mid-late High Grain Quality Rice ‘Jungmo1045’
Woo-Jae Kim, Woon-Chul Shin, Hyun-Su Park, Jeong-Kwon Nam, Hyun-Soon Kim, Man-Kee Baek, Bo-Kyeong Kim, Ki-Young Kim, Jeong-Ju Kim, Young-Chan Cho, Jae-Kwon Ko, Jong-Cheol Ko, So-Hyeon Back
Korean. J. Breed. Sci. 2018;50(1):72-79.   Published online March 1, 2018
DOI: https://doi.org/10.9787/KJBS.2018.50.1.72

‘Jungmo1045’, a multi-resistant japonica rice cultivar developed from a cross between ‘Hanggeumnuri’ having a good eating-quality and high yield and ‘SR30071’ having brown planthopper (BPH) resistance, was developed by the rice breeding team of NICS, RDA in 2015. This cultivar has about 121 days growth duration from transplanting to harvesting in west-southern coast, Honam and Youngnam plain of Korea. It has 73cm culm length and 22cm panicle length. In reaction to biotic and abiotic stresses, it shows resistance to bacterial blight pathogen races from K1 to K3, stripe virus and brown planthopper. The milled rice of ‘Jungmo1045’ exhibits translucent, relatively clear non-glutinous endosperm and medium short grain. It has good palatability of cooked rice compared with ‘Nampyeongbyeo’. The milled rice yield performance of this variety is about 5.74 MT/ha in local adaptability test for three years. ‘Junngmo1045’ would be useful genetic resources for multi-resistance breeding program against disease and insect and eco-friendly cultivation.

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벼흰잎마름병 저항성 고품질 중만생 벼 ‘만백’
Bacterial Blight Resistant Mid-late Maturing Rice ‘Manbaek’ with High Grain Quality
Hyun-Su Park, Man-Kee Baek, Bo-Kyeong Kim, Ki-Young Kim, Woon-Chul Shin, Jae-Kwon Ko, Jeong-Kwon Nam, Woo-Jae Kim, Young-Chan Cho, Jong-Cheol Ko, Jeong-Ju Kim, Hyun-Soon Kim
Korean. J. Breed. Sci. 2017;49(3):235-244.   Published online September 1, 2017
DOI: https://doi.org/10.9787/KJBS.2017.49.3.235

‘Manbaek’ is a bacterial blight resistant mid-late maturing rice cultivar with high grain quality. ‘Manbeak’ was derived from anther culture using the backcross combination, Hopum*2/SR30075. ‘Hopum’ is a mid-late maturing rice cultivar with high grain quality and ‘SR30075’ is a pyramid line carrying three bacterial blight resistance genes. ‘Manbaek’ was selected through the pedigree method, yield trials, and local adaptability tests. ‘Manbeak’ carrying two bacterial blight resistance gene Xa3 and xa5 showed high-level and broad-spectrum resistance against bacterial blight. ‘Manbaek’ was resistant to K3a, mostly virulent race in Korea, and exhibited resistance reaction against 16 Korean bacterial blight isolates. ‘Manbaek’ was a mid-late maturing rice. The heading date of ‘Manbaek’ was August 19th, which was 5 days later than that of ‘Nampyeong’. Manbaek’ was a lodging-tolerant rice with short culm and dark green leaf. Due to the low viviparous germination, ‘Manbaek’ could be a useful material to prevent pre-harvest sprouting. ‘Mabeak’ was resistant to bacterial blight and rice stripe virus, but susceptible to other virus diseases and insect pests. The yield of ‘Manbaek’ was similar to ‘Nampyeong’. ‘Manbaek’ showed excellent grain appearance and good tastes of cooked rice, so that it could contribute to improving the quality of bacterial blight resistant cultivars. ‘Manbaek’, bacterial blight resistant cultivar with high grain quality, is suitable for the cultivation at bacterial blight prone area and has been utilized in the breeding programs for enhancing the resistance against bacterial blight (Registration No. 6069).

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캘러스 활용도를 향상시키기 위한 벼( L.) 형질전환 시스템 구축
Development of rice(Oryza sativa L.) transformation system to improve callus utilization
Ji-Sun Park, Ki-Beom Moon, Jang-Ho Ha, Ji-Young Jang, Mi-Jin Kim, Jae-Heung Jeon, Sang-Un Park, Hyun-Soon Kim
Korean. J. Breed. Sci. 2017;49(3):170-179.   Published online September 1, 2017
DOI: https://doi.org/10.9787/KJBS.2017.49.3.170

Plant molecular farming has attracted a lot of attention lately in the field of mass production of industrially valuable materials by extending application of the plant as a kind of factory concept. Among them, protein expression system using rice(Oryza sativa L.) callus is a technology capable of mass culture and industrialization because of a high expression rate of a target protein. This study was carried out to develop an Agrobacterium-mediated transformation system to increase the utilization of rice callus. The transformation efficiency was improved by using the hand when seeds were de-husked for callus induction. Furthermore, we were possible induction of callus from 6 years old seed smoothly. Selection of the callus contained the target gene was required a cultivation period of at least 3 weeks, and the most efficient selection period was after 6 weeks of culture including one passage. This selection was confirmed that the gene was stably inserted into the genomic DNA of the plant cell by the southern blot analysis and progeny test. Such an efficient selection system of rice callus that can be cultured in the long term will be contribute to the industrialization of useful recombinant proteins using rice.

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