Fusarium wilt is a serious disease that causes damping-off, serious wilt symptoms, or death in melon. The aim of this study was to evaluate the resistance of melon germplasm to Fusarium wilt and select promising disease-resistant accessions for further applications. Resistance to Fusarium wilt was examined in 216 melon germplasms by the root-dipping inoculation method with Fusarium oxysporum f. sp. melonis fungal isolate KACC43206 (race 2). Inoculated seedlings were incubated for four weeks and assessed for disease symptoms using indices ranging from index 1 (no symptoms) to 5 (plant wither). A total of 34 accessions showed a disease index < 2.0. Disease indices of 10 accessions were 1.0 four weeks after inoculation. The resistant accessions of melon are expected to help in breeding commercial varieties with Fusarium wilt resistance.