This study was investigated to develop mass evaluation system for the contents of crude protein, oil and fatty acid in soybean germplasm using NIRS. NIRS equations were created with 345 soybeans, multiple correlation coefficients of crude protein, oil, palmitic, stearic, oleic, linoleic and linolenic acid between data obtained from NIRS and quantitative analysis were 0.983, 0.969, 0.592, 0.514, 0.978, 0.961 and 0.957, respectively. Equation statistics indicated that contents of crude protein, oil and unsaturated fatty acid except palmitic and stearic acid in soybean seed were suitable for determination by NIRS. Those NIRS equations were applied to examine crude protein, oil and unsaturated fatty acid of 854 soybean landraces from Korea. The average contents and ranges of crude protein and oil were 39.2% with a range of 33.7-47.0% and 15.0% with a range of 9.8-20.3%, individually. In addition, those of oleic, linoleic and linolenic acid were 21.4% with a range of 12.1-30.2%, 55.6% with a 47.8-62.3% and 8.1% with a range of 5.9-10.7% respectively. We conducted quantitative analysis to reconfirm with IT154552 (45.1%) and IT023955(46.9%) above 45% of crude protein, the results were similar from NIRS (45.2%, 47.0%). NIRS data for protein from this study made no difference with lab data, which would be useful for mass evaluation. There was negative correlation (-0.203) between crude protein and oil, positive correlation (0.379) between crude oil and oleic acid, and significantly negative correlation (-0.879) between oleic and linoleic acid.
This study was conducted to compare the agricultural characteristics of total 444 common bean accessions from Korea (296), China (76), and El Salvador (72). Days to flowering were ranged from 41 to 83 days with an average of 61 days. Days to sowing to maturing were ranged from 86 to 143 days with an average of 104 days. Common beans from El Salvador tend to bloom and mature 3 to 7 days earlier than Korea and China accessions. In growth habit, over 50% of Korea and China accessions were indeterminate and climbing type, but 90.1% from El Salvador were Semi-determinate and climbing type. Qualitative traits were much different among three countries. Eighty-two percentage of immature pod colors were dark pink from El Salvador, but many of those were pale yellow from Korea (77.6%) and China (61.8%). Seed shapes were divided into four types of round, oval, cubic and kidney type, and the highest percentage of those were 30.4% with kidney type. The highest of those was 36.6% with oval type from Korea, 55.3% with kidney type from China and 79.2% with cubic type from El Salvador. Morphological characteristics of common bean from El Salvador were much different from those of Korea and China, which is necessary to collect more germplasm from its native and expand genetic diversity of common beans. Four hundred thirty-five common beans from Korea, China and El Salvador were analyzed using SSR markers. Ninety-two alleles were detected with a lowest of 6 at the BM161, BM181 and a highest of 18 at the BM154, BM160. The average polymorphism information content (PIC) was 0.72. To similar with population size among three countries, 292 Korean accessions divided four replications with 73 accessions. As a result genetic diversity was the highest of 0.73 in Chinese populations, while the lowest of 0.48 in El Salvador populations.
This experiment was carried out to compare the morphological traits of 880 Korean soybean landraces from RDA genebank. Days to flowering were ranged from 51 to 104 days with an average of 74.4 days, days from flowering to maturity were ranged from 28 to 106 days with an average of 72.2 days, days from planting to maturity were ranged from 101 to 188 days with an average 146.6 days. Growth days were the shortest with 140.6 days in soybean landraces from Gangwon Province, and the longest with 152.8 days in those from Jeju Province. The 100 seed weight was ranged from 4.3 to 46.4 g with an average of 26.1 g, higher than other studies, relatively. The 100 seed weight was the lightest from Gyeongnam Province (22.7 g), and the heaviest from Chungnam Province (29.1 g). The highest percentage of seed coat color was black (52.4%), followed by yellow (28.5%). Landraces from Gyeongnam Province was the most yellow seed coat with 50.7%, seed coat color of those from Jeju was similar proportion in yellow (33.3%), green (25.9%) and black one (40.7%), and that from Gyeonggi was more green (28.8%) than yellow (16.7%). As a result of cotyledon color in 460 black seed was 59.7% with yellow, 40.1% with green. Three hundred fifty accessions of Korean soybean landraces were analyzed using 7 SSR markers. One hundred ten alleles were detected with a lowest 10 at the Satt307 and a highest 26 at the Satt173. The average polymorphism information content (PIC) was 0.71. Gene diversity was the highest in Jeju province while the lowest in Jeonnam.