‘Dian’ was developed by crossing ‘Milyang85/Suwon335’, which has tolerance to lodging and high yield, and ‘Milyang122’, which has good brewing qualities, in 2003. The regional yield trials were conducted for ‘Dian’ as a breeding line ‘Iksan168’ in four different regions of Korea from 2012 to 2014. The heading and maturing dates of ‘Dian’ in paddy and upland fields were similar to ‘Hopum’. ‘Dian’ had a shorter culm length than ‘Hopum’ in paddy and upland fields, showing tolerance to lodging stress. The yield potential of ‘Dian’ was approximately 18% higher than that of ‘Hopum’ in the paddy fields, but there were no significant differences in the upland fields. ‘Dian’ had rym1 and rym5 genes and showed resistance to barley yellow mosaic virus (BaYMV), but was susceptible to powdery mildew. It also had good quality for brewing and was similar to ‘Hopum’. ‘Dian’ had a higher assortment ratio (94%) than ‘Hopum’ (89%). Regarding grain quality, ‘Dian’ was similar to ‘Hopum’ in protein content, β-glucan content, and husk rate. Concerning malt quality, ‘Dian’ showed a 71.6% extraction rate, 3.6% soluble protein content, and 33.0% Kolbach index, which are similar to the values of ‘Hopum’. Other malt qualities of ‘Dian’ include 185 WK (Windisch-Kolbach) diastatic power and 81.4% friability, which were lower than those of ‘Hopum’. Therefore, ‘Dian’ with high yield, tolerance to lodging, and resistance to BaYMV is considered to be stable for cultivation (Registration No. 8241).

‘Heugho’, a new cultivar developed in 2014, is a two-rowed hulled barley with black grains that was selected among doubled haploid lines produced by anther culture from a backcross between two cultivars. Black barley, which has black grains, was used as the donor parent and ‘Hopum’, which has an elite genotype, was used as the recurrent parent. Agricultural characteristics of the new cultivar such as heading date, lodging and disease resistance were similar to those of ‘Hopum’. The yield was approximately 3.8 ton/ha, which was also comparable to that of ‘Hopum’. In terms of grain quality, the thousand grain weights of ‘Heugho’ and ‘Hopum’ were similar at 44.9 g and 41.4 g, respectively. The malt quality of both cultivars, which was mainly determined by extract, diastatic power, Kolbach index, and soluble protein content, were also similar. The total polyphenol contents of ‘Heugho’ and ‘Hopum’ malts were 0.274% and 0.244%, and the anthocyanin contents were 37.1 µg/g and 16.5 µg/g, respectively. After malting, ‘Heugho’ had high antioxidant capacity, making it a functional food source for beer as well as tea. We expect that ‘Heugho’ will contribute to farmer’s income and regional economic activation associated with the barley processing industry(Registration No. 8239).

A new malting barley variety, ‘Baegrok’, was developed in 2016 at the National Institute of Crop Science, RDA: Rural development administration by crossing the high-yield ‘Milyang130’ variety with ‘Myoginijo’, which has lodging tolerance and resistance to barley yellow mosaic virus (BaYMV). ‘Baegrok’ is suitable for use in brewing, similar to ‘Hopum’, and has resistance to powdery mildew and tolerance to lodging. From 2014 to 2016, regional yield trials were conducted in four different regions, with ‘Baegrok’ as a breeding line of ‘Iksan176’. The heading dates of ‘Baegrok’ in paddy and upland fields were April 6 and April 11, respectively, 2 days earlier than those of ‘Hopum’. Maturing dates of ‘Baegrok’ in paddy and upland fields were May 16 and May 21, respectively, similar to those of ‘Hopum’. The yield potential of ‘Baegrok’ was approximately 14% and 6% higher than that of ‘Hopum’ in upland and paddy fields. ‘Baegrok’ had a shorter culm length than ‘Hopum’ showing tolerance to lodging. Screening for powdery mildew resistance in the greenhouse showed that ‘Baegrok’ had resistance to powdery mildew, unlike ‘Hopum’ which was susceptible. It also is suitable for use in brewing, similar to Hopum. ‘Baegrok’ showed good grain quality in terms of β-glucan content (3.4%), starch (61.0%), and husk rate (12.7%). Regarding malt quality, ‘Baegrok’ exhibited 75.1% acrospire length, 70.8% friability, 4.4% soluble protein, and 345.8 WK (Windisch-Kolbach) diastatic power, superior to the malt quality of ‘Hopum’. Thus, ‘Baegrok’ is resistant to powdery mildew, tolerant to lodging, and more suitable for brewing. (Registration No. 8399)

A malting barley variety, ‘Nurimaeg’, was developed with resistance to barley yellow mosaic virus (BaYMV), tolerance to lodging stress, and good brewing quality. ‘Nurimaeg’ was developed by crossing Milyang127 and Miharu gold in 2004. Regional yield trials were conducted for ‘Nurimaeg’ as a breeding line ‘Iksan175’ in four different regions from 2014-2016. It has a type I growth habit, and its average heading and maturity dates were April 12 and May 20, respectively-which were 1 day later than the maturity date of ‘Hopum’ variety. As ‘Nurimaeg’ has a shorter culm length (77 cm) than that of ‘Hopum’, it showed tolerance to lodging stress. This variety possesses the rym5 gene and is resistant to BaYMV. It showed a 42.9 g 1000-grain weight and 90% assortment ratio. The yield potential of ‘Nurimaeg’ was 408 kg/10a, which is approximately 18% higher than that of ‘Hopum’. Analysis of grain quality for malting showed that ‘Nurimaeg’ has high quality of low protein and β-glucan content. Malt quality analysis revealed that it has 72.8% extract, 4.2% soluble protein, 38.9% kolbach index, and 243 WK diastatic power, which all were similar to those of ‘Hopum’. ‘Nurimaeg’ would be suitable for cultivation in the areas of Korea that have a daily minimum temperature of -4℃ in the month of January (Registration No. 7960).

Barley is an economically important cereal crop grown under diverse environmental conditions and ranked fourth in terms of production volume. Barley is a diploid self-fertilizing plant with seven chromosomes, and has a 5.1 Gbp genome with more than 80% repeat sequences. Whole genome re-sequencing (WGR) has provided substantial information on sequence variation distributed on all chromosomes, such as single nucleotide polymorphisms, insertions, and deletions, which are used in the development of DNA markers. In this study, we performed WGR to detect sequence variations among six Korean malting varieties. An average of 92,552 insertions and deletions (InDels) were detected in these varieties in comparison to the high-quality reference genome sequences. The InDel density of the six Korean malting varieties ranged from 17 to 19 InDel/1Mbp with an average of 18 InDel/1Mbp. No InDel could be detected in 193 regions in all chromosomes except chr. unassigned. One interval with high-density InDel, more than 150 InDel/1Mbp, was located on the 1H, 3H, 6H, and 7H chromosomes. A total of 145 InDel markers were developed using 225 large-InDel markers, longer than 50 bp. Seventeen large-InDel makers showed polymorphisms among 31 malting barley varieties. These 31 malting barley varieties were divided into four groups based on phylogenetic analysis. These results presented a development method of agarose-resolvable large-InDel markers using WGR. Seventeen polymorphic large-InDel markers were used to conserve and identify barley germplasms. This vast information on sequence variation in six Korean malting barleys could be used for the development of DNA markers and marker-assisted selection.

We developed a malting barley, ‘Dapum’, with stable yield and enhanced quality for brewing. ‘Dapum’ was developed in 2015, by crossing Hopum, which has high brewing quality, and ‘GobDH96’, which has steady yield. The regional yield trials were conducted for ‘Dapum’ as a breeding line ‘Iksan173’ in four different regions from 2013 to 2015. It has a type I growth habit, and the average heading and maturing date in paddy fields were April 20 and May 26, respectively, which were 1 day earlier than those of ‘Hopum’. The yield potential of ‘Dapum’ was 411 kg/ha, which is approximately 10% higher than those of ‘Hopum’ under paddy field condition. ‘Dapum’ possesses the rym5 gene and is resistant to barley yellow mosaic virus (BaYMV), although susceptible to powdery mildew and lodging tolerance. It is similar to ‘Hopum’ in terms of enhanced brewing quality. ‘Dapum’ had a higher 1,000-grain weight (46.1 g) and assortment ratio (92%) than those of ‘Hopum,’ at 43.8 g and 90%, respectively. Analysis of grain quality for malting showed that ‘Dapum’ has high quality protein content, β-glucan content, and water sensitivity. Malt quality analysis revealed that ‘Dapum’ has 73.3% extraction rate, 3.7% soluble protein content, 32.6% Kolbach index, 244 WK (Windisch-Kolbach) diastatic power, and 80.2% friability, which were similar to those of ‘Hopum’. It would be suitable for cultivation in the zones of Korea that have a daily minimum temperature of -4℃ in January (Registration No. 7512).