The purpose of this research is a molecular characterization of introduced T-DNA in transgenic rice T4~T6 generationlines (#8) harboring a pepper MsrB2 gene under the control of stress inducible Rab21 promoter, as a part of biosafety evaluationfor drought-tolerant transgenic rice (CaMsrB2-8). We identified the structure and sequence of transformation vector of T-DNAand analyzed insertion sites, flanking sequences, and generational stability of inserted T-DNA in transgenic rice. The transformationvector was consisted of right border, a drought-tolerant CaMsrB2-8 gene unit (Rab21 promoter::CaMsrB2::PinII terminator), aselectable marker herbicide resistance unit (CaMV 35S promoter::bar::Nos terminator), and left border in sequential order. Weconfirmed that T-DNA was introduced at the position of 41,737,284 bp of chromosome No. 1. based on the adaptor-ligation PCRand whole genome sequence database. T-DNAs were stably inherited through the T4 to T6 generations, and also stable expressionof bar gene from T-DNA was confirmed. It was also confirmed that the backbone DNA of transformation vector containingantibacterial gene (aadA) was not present in CaMsrB2-8 rice genome. These results will be filed to biosafety assessment documentof CaMsrB2-8 rice.
