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자포니카 다수성 벼 드래찬과 보람찬 유래 약배양 계통을 이용한 이삭 및 수량 관련 형질 분석 및 다수성 계통 육성

Development of High-yielding Rice Lines and Analysis of Panicle and Yield-related Traits Using Doubled Haploid Lines Derived from the Cross between Deuraechan and Boramchan, High-yielding japonica Rice Cultivars in Korea

Korean Journal of Breeding Science 2015;47(4):384-402.
Published online: November 30, 2015

1 농촌진흥청 국립식량과학원

1 National Institute of Crop Science, RDA, Wanju 55365, Korea

2 농촌진흥청 국립식량과학원 중부작물부

2 Department of Central Area, NICS, RDA, Suweon 16429, Korea

3 충남대학교 농업생명대학

3 Department of Agronomy, Chungnam National University, Daejeon 34134, Korea

*Corresponding author (mayoe@korea.kr, Tel: +82-63-238-5202, Fax: +82-63-238-5205)
• Received: October 12, 2015   • Revised: November 18, 2015   • Accepted: November 18, 2015

© Korean Society of Breeding Science All rights reserved

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Development of High-yielding Rice Lines and Analysis of Panicle and Yield-related Traits Using Doubled Haploid Lines Derived from the Cross between Deuraechan and Boramchan, High-yielding japonica Rice Cultivars in Korea
Korean. J. Breed. Sci.. 2015;47(4):384-402.   Published online December 31, 2015
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Development of High-yielding Rice Lines and Analysis of Panicle and Yield-related Traits Using Doubled Haploid Lines Derived from the Cross between Deuraechan and Boramchan, High-yielding japonica Rice Cultivars in Korea
Korean. J. Breed. Sci.. 2015;47(4):384-402.   Published online December 31, 2015
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Development of High-yielding Rice Lines and Analysis of Panicle and Yield-related Traits Using Doubled Haploid Lines Derived from the Cross between Deuraechan and Boramchan, High-yielding japonica Rice Cultivars in Korea
Image Image Image Image Image Image
Fig. 1. Relationship among traits in the principal component analysis (PCA) diagram composed of component 1 and 2 in 2011 (A) and 2012 (B). Abbreviations are listed in table 1.
Fig. 2. Distribution of panicle and yield-related traits in 2012. PL (A), TNN (B), NR (C), NS (D), NPr (E), NSPr (F), MNSPr (G), NSr (H), NSSr (I), MNSSr (J), Sr/Pr (K), SSr/ SPr (L), CL (M), FL (N), PN (O), TGW (P), RRG (Q), Yield (R). Abbreviations are listed in table 1.
Fig. 3. Cluster analysis of DH lines based on panicle and yield-related traits in 2012.
Fig. 4. Relationship among traits (A) and lines (B) in the principal component analysis (PCA) diagram composed of component 1 and 2 in 2013. Abbreviations are listed in table 1 and table 6.
Fig. 5. Path analysis of causal relationships among traits to explain the variation of yield in 2012 (A) and 2013 (B). Continuous arrows and dotted arrows indicate the significant and no significant effect, respectively. The arrow line thickness means proportion of the effect. ** and * mean significant at 1 and 5% probability level, respectively.
Fig. 6. Pedigree diagram of Deuraechan.
Development of High-yielding Rice Lines and Analysis of Panicle and Yield-related Traits Using Doubled Haploid Lines Derived from the Cross between Deuraechan and Boramchan, High-yielding japonica Rice Cultivars in Korea

The abbreviations for panicle and yield-related traits.

Abbreviations:

PL Panicle length
TNN Thickness of neck node
PRB Primary rachis-branch
SRB Secondary rachis-branch
NR Number of rachis-branches per panicle
NS Number of spikelets per panicle
NPr Number of PRBs per panicle
NSPr Number of spikelets on PRBs per panicle
MNSPr Mean number of spikelets on a PRB
NSr Number of SRBs per panicle
NSSr Number of spikelets on SRBs per panicle
MNSSr Mean number of spikelets on a SRB
Sr/Pr Ratio of SRBs to PRBs
SSr/SPr Ratio of spikelets on SRBs to spikelets on PRBs
CL Culm length
FL Flag leaf length
PN Panicle number per hill
TGW 1,000 grain weight
RRG Ratio of ripened grain
BRR Brown/rough rice ratio
RRY Rough rice yield
BRY Brown rice yield
MRY Milled rice yield
DAS Day after seedling

Anther culture ability and doubled haploid lines derived from the cross of Deuraechan/Boramchan.

Anther cultured (no.) Cali induced (no. and %) Plant regeneration (no. and %)z Green plants (no. and %)y Albino (no. and %)x Doubled haploid lines (no.)w

10,600 3,528(33.3) 450(12.8) 245(54.4) 215(45.6) 220

zPercent plant regeneration is the number of plants regenerated over number of cali plated

yPercent green is the number of green plants regenerated over total plants regenerated

xPercent albino is the number of albino plants regenerated over total plants regenerated

wDouble haploid lines are harvested plants having fertile grain

Correlation coefficient among panicle and yield related traits in 2011 and 2012 (n=163).

Traitsz PLy TNN NR NS NPr NSPr MNSPr NSr NSSr MNSSr Sr/Pr SSr/SPr CL FL PN TGW RRG Yield
(x1) (x2) (x3) (x4) (x5) (x6) (x7) (x8) (x9) (x10) (x11) (x12) (x13) (x14) (x15) (x16) (x17) (x18)

x1 0.547** 0.472** 0.515** 0.544** 0.589** 0.354** 0.415** 0.396** 0.229** 0.241** 0.209** 0.209** 0.556** -0.392** 0.381** -0.210** 0.198*
x2 0.752** 0.858** 0.884** 0.655** 0.674** 0.294** 0.816** 0.803** 0.549** 0.629** 0.602** 0.005ns 0.513** -0.325** 0.159* -0.313** 0.376**
x3 0.706** 0.833** 0.987** 0.571** 0.571** 0.188* 0.988** 0.965** 0.587** 0.859** 0.823** -0.052ns 0.412** -0.302** 0.044ns -0.364** 0.468**
x4 0.659** 0.846** 0.990** 0.609** 0.630** 0.286** 0.967** 0.958** 0.640** 0.820** 0.793** -0.039ns 0.442** -0.297** 0.059ns -0.378** 0.456**
x5 0.794** 0.765** 0.765** 0.817** 0.966** 0.218** 0.437** 0.367** 0.002ns 0.075ns 0.031ns 0.194* 0.476** -0.343** 0.395** -0.156* 0.118ns
x6 0.803** 0.745** 0.746** 0.812** 0.979** 0.462** 0.443** 0.380** 0.050ns 0.093ns 0.033ns 0.224** 0.515** -0.347** 0.388** -0.170* 0.126ns
x7 0.561** 0.424** 0.423** 0.516** 0.579** 0.732** 0.165* 0.169* 0.185* 0.086ns 0.009ns 0.171* 0.325** -0.128ns 0.118ns -0.098ns 0.07ns
x8 0.594** 0.799** 0.991** 0.969** 0.674** 0.656** 0.367** 0.988** 0.643** 0.926** 0.896** -0.094ns 0.361** -0.266** -0.026ns -0.370** 0.491**
x9 0.580** 0.792** 0.982** 0.966** 0.649** 0.633** 0.360** 0.994** 0.744** 0.942** 0.933** -0.130ns 0.335** -0.226** -0.073ns -0.388** 0.497**
x10 0.401** 0.610** 0.739** 0.759** 0.418** 0.417** 0.281** 0.762** 0.821** 0.713** 0.785** -0.267** 0.180* 0.010ns -0.283** -0.347** 0.382**
x11 0.298** 0.590** 0.825** 0.770** 0.288** 0.256** 0.058ns 0.888** 0.907** 0.806** 0.986** -0.182* 0.204** -0.164ns -0.194* -0.354** 0.495**
x12 0.346** 0.620** 0.862** 0.807** 0.338** 0.324** 0.161* 0.921** 0.927** 0.757** 0.986** -0.222** 0.166* -0.119ns -0.234** -0.369** 0.492**
x13 0.061ns -0.160* -0.221** -0.194ns 0.060ns 0.079ns 0.114ns -0.266** -0.293** -0.424** -0.415** -0.376** -0.075ns -0.047ns 0.386** 0.045ns -0.100ns
x14 0.549** 0.432** 0.413** 0.421** 0.420** 0.426** 0.289** 0.388** 0.369** 0.189* 0.237** 0.279** -0.006ns -0.418** 0.285** -0.163* 0.241**
x15 -0.807** -0.762** -0.733** -0.750** -0.726** -0.713** -0.417** -0.693** -0.678** -0.498** -0.478** -0.515** 0.148ns -0.548** -0.361** 0.091ns 0.142ns
x16 0.314** 0.043ns -0.088ns -0.055ns 0.200* 0.194* 0.107ns -0.142ns -0.158* -0.252** -0.310** -0.291** 0.322** 0.375** -0.180* 0.405** -0.080ns
x17 -0.329** -0.398** -0.538** -0.527** -0.321** -0.308** -0.162* -0.552** -0.562** -0.511** -0.550** -0.545** 0.317** -0.164* 0.378** 0.458** -0.169*
x18 0.196* 0.261** 0.410** 0.413** 0.270** 0.271** 0.187* 0.415** 0.427** 0.427** 0.402** 0.400** -0.14ns 0.254** -0.202** 0.051ns -0.323**

zUpper and lower diagonal indicate the value in 2011 and 2012, respectively

yAbbreviations are listed in table 1

Eigenvectors for the principal components of traits and correlation coefficient between the traits and principal components.

Trait 2011 2012

Eigenvector Correlation coefficient Eigenvector Correlation coefficient

Prin1z Prin2 Prin1 Prin2 Prin1z Prin2 Prin1 Prin2

PLy 0.197 0.277 0.567** 0.511** 0.236 0.299 0.743** 0.516**
TNN 0.311 0.102 0.895** 0.189* 0.276 0.092 0.870** 0.159*
NR 0.339 -0.015 0.975** -0.028ns 0.310 -0.036 0.979** -0.062ns
NS 0.343 0.012 0.987** 0.023ns 0.313 0.010 0.987** 0.017ns
NPr 0.193 0.369 0.554** 0.680** 0.249 0.280 0.786** 0.484**
NSPr 0.202 0.385 0.582** 0.709** 0.248 0.303 0.781** 0.523**
MNSPr 0.102 0.191 0.292** 0.352** 0.158 0.276 0.498** 0.476**
NSr 0.335 -0.086 0.964** -0.158* 0.305 -0.099 0.963** -0.171*
NSSr 0.334 -0.128 0.960** -0.235** 0.305 -0.121 0.962** -0.209**
MNSSr 0.235 -0.252 0.676** -0.465** 0.247 -0.212 0.779** -0.366**
Sr/Pr 0.292 -0.248 0.841** -0.456** 0.249 -0.316 0.787** -0.545**
SSr/SPr 0.284 -0.285 0.816** -0.525** 0.260 -0.275 0.820** -0.474**
CL -0.023 0.256 -0.067ns 0.472** -0.079 0.330 -0.249** 0.570**
FL 0.178 0.236 0.511** 0.436** 0.153 0.220 0.484** 0.381**
PN -0.116 -0.235 -0.333** -0.434** -0.254 -0.167 -0.801** -0.288**
TGW 0.016 0.394 0.045ns 0.726** -0.017 0.416 -0.053ns 0.718**
RRG -0.148 0.110 -0.425** 0.203** -0.184 0.215 -0.580** 0.370**
Yield 0.176 -0.133 0.507** -0.245** 0.141 -0.072 0.446** -0.125ns
Eigen value 8.27 3.40 9.95 2.98
Contribution (%) 46.0 18.9 55.3 16.6
Cumulative contribution (%) 46.0 64.8 55.3 71.8

zPrin1 and Prin2 indicate the first and second component, respectively

yAbbreviations are listed in table 1

Average and range of traits value of the parents and groups divided by cluster analysis in 2012.

Group n PLz (cm) TNN (mm) NR NS NPr NSPr MNSPr NSr NSSr MNSSr Sr/Pr SSr/SPr CL (cm) FL (cm) PN TGW (g) RRG (%) Yield (g)

Deuraechan 3 26.3ay 1.74a 42a 166a 14a 86a 6.1a 28a 80a 2.9a 1.9ab 0.9bc 69.1a 32.4a 9c 25.0a 87.3ab 535bc
Boramchan 3 21.0d 1.53bc 35bc 129b 11d 60d 5.7d 24ab 69ab 2.8ab 2.3a 1.1a 67.6a 30.3b 11a 24.3a 91.3a 551b
a 108 24.7ab 1.63ab 40ab 156a 13b 79b 6.0ab 27a 78a 2.9a 2.1ab 1.0abc 68.7a 32.3a 9bc 24.2a 84.1b 520c
(20.1~27.1) (1.29~1.88) (26~51) (107~197) (11~15) (65~93) (5.7~6.3) (15~38) (41~118) (2.6~3.1) (1.3~3.1) (0.6~1.6) (62.3~74.3) (28.3~35.7) (8~13) (21.7~27.2) (74.1~93.0) (477~554)
b 42 22.6cd 1.49c 33c 127b 12c 69c 5.8c 21b 58b 2.7b 1.8b 0.8c 69.5a 31.0ab 11ab 24.0a 87.3ab 467d
(19.2~26.5) (1.32~1.77) (24~44) (97~172) (10~14) (57~85) (5.6~6.1) (13~31) (35~90) (2.5~3) (1.2~2.6) (0.5~1.3) (63.5~75.0) (26.9~33.5) (9~12) (21.0~27.1) (69.4~94.9) (410~501)
c 13 23.7bc 1.59bc 40ab 156a 13bc 75bc 5.9bc 28a 81a 2.9a 2.2a 1.1ab 68.2a 31.8ab 10abc 24.2a 83.9c 581a
(20.7~26.5) (1.25~1.80) (27~46) (108~179) (11~14) (62~85) (5.7~6.1) (16~33) (42~102) (2.7~3.1) (1.4~2.7) (0.6~1.4) (63.9~72.1) (28.8~35.0) (8~13) (21.9~25.7) (75.2~92.4) (555~629)
Total 163 24.1 1.59 38.2 149 13 76 5.9 26 73 2.8 2.0 1.0 68.8 31.9 10 24.1 84.9 512
C.V.(%)x 163 8.3 8.2 15.4 15.1 8.7 10.3 3.4 20.0 23.0 3.6 15.0 20.0 3.5 5.0 11.3 4.5 5.3 7.2

zAbbreviations are listed in table 1

yMeans with same letter are not significantly different at p < 0.05 (ANOVA followed by DMRT)

xC.V. = coefficient of variation

Panicle and yield-related traits of parents and selected DH lines at yield trial in 2013.

Var./lines PLz (cm) TNN NR NS NPr NSPr MNSPr NSr NSSr MNSSr Sr/Pr SSr/SPr CL (cm) FL PN TGW (g) RRG (%) MRY (kg/10a)

Deuraechan (DR) 26aby 1.76ab 32cde 122bcd 11abcd 67bcd 5.9bcde 20de 55efgh 2.7ef 1.7ef 0.8ghi 73ab 36.0ab 10efg 25.3bc 91.2a 512b
Boramchan (BR) 21e 1.50def 31cde 111de 9h 51h 5.5h 22cde 60def 2.8cde 2.3abc 1.2bc 71bcd 32.1de 13abc 24.0ef 91.4a 567ab
HR28832-AC60-1-1 23cd 1.62bcde 31cde 117bcde 10fgh 56gh 5.8defg 21cde 61def 2.8c 2.2bcd 1.1cd 72bc 34.2abcde 12bcd 23.7fg 91.9a 596a
(AC60)
HR28832-AC105-1-1 24cd 1.69abc 35abc 132abc 11bcde 65cde 5.8cdefg 23bcd 67bcde 2.8c 2.0de 1.0def 68e 33.5bcde 12abcd 24.3e 84.1bcd 559ab
(AC105)
HR28832-AC107-1-1 26ab 1.68abc 39a 149a 12a 73a 6.0ab 27ab 75ab 2.8cd 2.2bcd 1.0de 73ab 37.2a 9h 25.7ab 83.8bcd 516b
(AC107)
HR28832-AC109-1-1 23de 1.34g 26f 101e 10gh 57g 5.9abc 16f 44h 2.7ef 1.6fg 0.7hi 72bc 31.0e 13ab 24.5de 89.4ab 561ab
(AC109)
HR28832-AC112-1-1 23de 1.47efg 31cde 117bcde 11def 60gef 5.7fg 21de 57efg 2.7def 1.9def 0.9efg 71bcd 32.0de 12cde 23.2g 92.2a 549ab
(AC112)
HR28832-AC123-1-1 27a 1.62bcde 34abc 133ab 12abc 71abc 6.0a 23cd 62cdef 2.8cdef 1.9def 0.9fgh 73abc 32.6cde 10gh 25.7ab 85.3bcd 541ab
(AC123)
HR28832-AC130-1-1 25ab 1.66abc 29ef 115cde 12abcd 67bcd 5.8cdef 18ef 47gh 2.7ef 1.5g 0.7i 73ab 34.1abcde 10fgh 25.5abc 87.6abc 516b
(AC130)
HR28832-AC134-1-1 26ab 1.58cdef 37ab 143a 12ab 72ab 6.0a 25abc 71abcd 2.8bc 2.0cd 1.0def 75a 35.8abc 10gh 25.0cd 82.7cd 500b
(AC134)
HR28832-AC149-1-1 23cd 1.62bcde 35abc 132abc 10efg 58gf 5.7g 25abc 74abc 2.9ab 2.4ab 1.2ab 68de 31.3e 11cdef 23.3g 85.0bcd 564ab
(AC149)
HR28832-AC152-1-1 23cd 1.65abcd 38a 148a 11cde 66cde 6.0ab 27ab 82a 3.0a 2.4ab 1.2ab 72bcd 35.9ab 11cdef 24.0ef 89.5ab 595a
(AC152)
HR28832-AC156-1-1 22de 1.46fg 29def 110de 10fgh 56g 5.7efg 20def 54fgh 2.7cdef 2.0de 0.9defg 71bcd 35.5abc 14a 23.7fg 90.9a 590a
(AC156)
HR28832-AC161-1-1 25bc 1.73abc 38a 146a 11de 63def 5.9bcd 28a 83a 3.0a 2.6a 1.3a 69de 35.1abcd 11defg 25.5abc 84.9bcd 605a
(AC161)
HR28832-AC178-1-1 25abc 1.80a 33bcde 122bcd 11cde 63def 5.8cdefg 22cd 58efg 2.7f 2.0de 0.9defg 70cde 33.8bcde 10gh 26.1a 80.7d 507b
(AC178)
Mean 24 1.61 33 125 11 62 5.8 62 22 2.8 2.0 1.0 71 34 11 24.6 87.5 552
C.V. 7.5 8.7 27.3 28.0 18.2 21.0 5.2 36.4 40.3 7.1 30.0 30.0 3.5 6.9 13.5 4.1 5.3 8.2

zAbbreviations are listed in table 1

yMeans with same letter are not significantly different at p < 0.05 (ANOVA followed by DMRT)

Correlation coefficient among panicle-related traits and agronomic traits in 2013 (n=15).

Traits PLz TNN NR NS NPr NSPr MNSPr NSr NSSr MNSSr Sr/Pr SSr/SPr CL FL PN TGW RRG MRY
(x1) (x2) (x3) (x4) (x5) (x6) (x7) (x8) (x9) (x10) (x11) (x12) (x13) (x14) (x15) (x16) (x17) (x18)

x1 0.612* 0.464ns 0.558* 0.902** 0.907** 0.728** 0.279ns 0.218ns -0.008ns -0.265ns -0.213ns 0.420ns 0.473ns -0.918** 0.802** -0.531* -0.647**
x2 0.568* 0.564* 0.584* 0.519* 0.209ns 0.483ns 0.443ns 0.251ns 0.197ns 0.210ns -0.233ns 0.486ns -0.684** 0.571* -0.457ns -0.289ns
x3 0.984** 0.581* 0.582* 0.423ns 0.975** 0.952** 0.761** 0.647** 0.692** -0.109ns 0.523* -0.570* 0.232ns -0.518* 0.010ns
x4 0.671** 0.687** 0.560* 0.932** 0.914** 0.739** 0.543* 0.588* -0.023ns 0.554* -0.619* 0.301ns -0.532* -0.040ns
x5 0.987** 0.713** 0.384ns 0.320ns 0.059ns -0.232ns -0.182ns 0.341ns 0.549* -0.882** 0.660** -0.548* -0.650**
x6 0.815** 0.390ns 0.334ns 0.092ns -0.232ns -0.175ns 0.393ns 0.558* -0.847** 0.663** -0.539* -0.591*
x7 0.285ns 0.272ns 0.164ns -0.212ns -0.143ns 0.450ns 0.463ns -0.536* 0.541* -0.375ns -0.247ns
x8 0.992** 0.847** 0.798** 0.835** -0.217ns 0.442ns -0.404ns 0.082ns -0.437ns 0.190ns
x9 0.907** 0.835** 0.861** -0.249ns 0.407ns -0.330ns 0.021ns -0.390ns 0.278ns
x10 0.871** 0.857** -0.301ns 0.244ns -0.047ns -0.211ns -0.164ns 0.540*
x11 0.992** -0.471ns 0.102ns 0.108ns -0.318ns -0.103ns 0.598*
x12 -0.426ns 0.157ns 0.060ns -0.269ns -0.148ns 0.563*
x13 0.354ns -0.328ns 0.249ns 0.187ns -0.468ns
x14 -0.466ns 0.380ns -0.160ns -0.158ns
x15 -0.743** 0.551* 0.679**
x16 -0.579* -0.584*
x17 0.409ns

zAbbreviations are listed in table 1

Eigenvectors for the principal components of traits and correlation coefficient between the traits and principal components.

Trait Eigenvector Correlation coefficient

Prin1z Prin2 Prin3 Prin1 Prin2 Prin3

PL 0.275 -0.230 -0.011 0.789** -0.551* -0.014ns
TNN 0.246 -0.015 -0.375 0.707** -0.037ns -0.451ns
NR 0.313 0.172 0.030 0.898** 0.412ns 0.036ns
NS 0.326 0.127 0.087 0.937** 0.306ns 0.105ns
NPr 0.292 -0.198 0.027 0.838** -0.476ns 0.032ns
NSPr 0.293 -0.195 0.108 0.842** -0.468ns 0.130ns
MNSPr 0.228 -0.150 0.342 0.653** -0.359ns 0.411ns
NSr 0.275 0.249 0.027 0.788** 0.598* 0.032ns
NSSr 0.260 0.274 0.053 0.745** 0.659** 0.064ns
MNSSr 0.176 0.329 0.145 0.506ns 0.791** 0.174ns
Sr/Pr 0.103 0.389 -0.036 0.297ns 0.933** -0.043ns
SSr/SPr 0.123 0.377 -0.014 0.352ns 0.906** -0.016ns
CL 0.040 -0.245 0.569 0.114ns -0.589* 0.684**
FL 0.221 -0.034 0.313 0.635* -0.082ns 0.376ns
PN -0.287 0.178 0.112 -0.825** 0.427ns 0.134ns
TGW 0.203 -0.241 -0.227 0.582* -0.579* -0.273ns
RRG -0.225 0.044 0.422 -0.645** 0.105ns 0.507ns
MRY -0.109 0.331 0.181 -0.312ns 0.795** 0.218ns
Eigen value 8.24 5.77 1.45
Contribution (%) 45.8 32.0 0.08
Cumulative contribution (%) 45.8 77.8 85.8

zPrin1 and Prin2 indicate the first and second component, respectively

Yield-related traits of parents and selected DH lines at yield trial in 2014.

Var./lines Heading date (DASz) CL (cm) PL (cm) PN NS RRG (%) TGW (g) RRY (kg/10a) BRR (%) BRY (kg/10a) MRY (kg/10a) Index of MRY (%)

Deuraechan (DR) 107ay 69a 24a 9c 142a 91.1ab 25.8a 641b 81.6b 523b 481b 100
Boramchan (BR) 108a 69a 20c 12a 108b 94.5a 24.4b 736a 82.5ab 607a 558a 116
HR28832-AC60-1-1 108a 66bc 22b 11b 129ab 91.0ab 24.5b 772a 81.0b 625a 575a 119
(AC60)
HR28832-AC152-1-1 108a 68ab 22b 11b 140a 90.5ab 24.7b 744a 81.6b 607a 558a 116
(AC152)
HR28832-AC156-1-1 108a 64c 20c 12a 108b 94.8a 24.0c 741a 83.2a 617a 567a 118
(AC156)
HR28832-AC161-1-1 106a 65bc 22b 11b 143a 86.3b 25.9a 748a 81.1b 607a 559a 116
(AC161)

zAbbreviations are listed in table 1

yMeans with same letter are not significantly different at p < 0.05 (ANOVA followed by DMRT)

Table 1. The abbreviations for panicle and yield-related traits.
Table 2. Anther culture ability and doubled haploid lines derived from the cross of Deuraechan/Boramchan.

Percent plant regeneration is the number of plants regenerated over number of cali plated

Percent green is the number of green plants regenerated over total plants regenerated

Percent albino is the number of albino plants regenerated over total plants regenerated

Double haploid lines are harvested plants having fertile grain

Table 3. Correlation coefficient among panicle and yield related traits in 2011 and 2012 (n=163).

Upper and lower diagonal indicate the value in 2011 and 2012, respectively

Abbreviations are listed in table 1

Table 4. Eigenvectors for the principal components of traits and correlation coefficient between the traits and principal components.

Prin1 and Prin2 indicate the first and second component, respectively

Abbreviations are listed in table 1

Table 5. Average and range of traits value of the parents and groups divided by cluster analysis in 2012.

Abbreviations are listed in table 1

Means with same letter are not significantly different at p < 0.05 (ANOVA followed by DMRT)

C.V. = coefficient of variation

Table 6. Panicle and yield-related traits of parents and selected DH lines at yield trial in 2013.

Abbreviations are listed in table 1

Means with same letter are not significantly different at p < 0.05 (ANOVA followed by DMRT)

Table 7. Correlation coefficient among panicle-related traits and agronomic traits in 2013 (n=15).

Abbreviations are listed in table 1

Table 8. Eigenvectors for the principal components of traits and correlation coefficient between the traits and principal components.

Prin1 and Prin2 indicate the first and second component, respectively

Table 9. Yield-related traits of parents and selected DH lines at yield trial in 2014.

Abbreviations are listed in table 1

Means with same letter are not significantly different at p < 0.05 (ANOVA followed by DMRT)